Rabbit Anti-NDFIP1 Polyclonal Antibody – Cat# MBS6324235

NDFIP1 Antibody (Center) western blot analysis in human normal Uterus tissue lysates (35ug/lane).This demonstrates the NDFIP1 antibody detected the NDFIP1 protein (arrow).

NDFIP1 Antibody – Rabbit Polyclonal

Name: NDFIP1 Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS6324235
Price: 1060 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6324235

The protein encoded by this gene belongs to a small group of evolutionarily conserved proteins with three transmembrane domains. It is a potential target for ubiquitination by the Nedd4 family of proteins. This protein is thought to be part of a family of… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

NDFIP1, Polyclonal Antibody

Full Product Name

NDFIP1, ID (NDFIP1, N4WBP5, NEDD4 family-interacting protein 1, Breast cancer-associated protein SGA-1M, NEDD4 WW domain-binding protein 5, Putative MAPK-activating protein PM13, Putative NF-kappa-B-activating protein 164, Putative NFKB and MAPK-activatin

Product Gene Name

anti-NDFIP1 antibody

Product Synonym Names

NDFIP1; N4WBP5; NEDD4 family-interacting protein 1; Breast cancer-associated protein SGA-1M; NEDD4 WW domain-binding protein 5; Putative MAPK-activating protein PM13; Putative NF-kappa-B-activating protein 164; Putative NFKB and MAPK-activating protein

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human

Form/Format

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

Purity/Purification

Purified by Protein A Affinity Chromatography.

Preparation and Storage

Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Molecular Weight

24,899 Da

Chromosome Location

Chromosome: 5; NC_000005.9 (141488324..141534008). Location: 5q31.3

OMIM

612050

3D Structure

ModBase 3D Structure for Q9BT67

Immunogen

NDFIP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 76-106 amino acids from the Central region of human NDFIP1.

Conjugate

FITC

Note

Preservative Free

Special Handling

Light sensitive

Product Categories/Family for anti-NDFIP1 antibody

Antibodies; Cancer Markers

Applications Tested/Suitable for anti-NDFIP1 antibody

Western Blot (WB), FLISA

Application Notes for anti-NDFIP1 antibody

WB: 1:100-500
FLISA: 1:1,000
Applications are based on unconjugated antibody.

Product References and Citations for anti-NDFIP1 antibody

Mund, T., et al. Proc. Natl. Acad. Sci. U.S.A. 107(25):11429-11434(2010). Howitt, J., et al. Proc. Natl. Acad. Sci. U.S.A. 106(36):15489-15494(2009). Rapley, E.A., et al. Nat. Genet. 41(7):807-810(2009). Mund, T., et al. EMBO Rep. 10(5):501-507(2009). Putz, U., et al. J. Biol. Chem. 283(47):32621-32627(2008).

Other Notes

Small volumes of anti-NDFIP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NDFIP1 purchase

MBS6324235 is an antibody targeting NDFIP1. Also known as NDFIP1, ID (NDFIP1, N4WBP5, NEDD4 family-interacting protein 1, Breast cancer-associated protein SGA-1M, NEDD4 WW domain-binding protein 5, Putative MAPK-activating protein PM13, Putative NF-kappa-B-activating protein 164, Putative NFKB and MAPK-activatin. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for NDFIP1. It may not necessarily be applicable to this product.

NCBI GI #

13386480

NCBI GeneID

80762

NCBI Accession #

NP_085048.1

UniProt Primary Accession #

Q9BT67

UniProt Secondary Accession #

Q658T8; Q8N2E3; Q8N2F9; B2RDB8; D3DQF0

UniProt Related Accession #

Q9BT67

GenBank Nucleotide #

NM_030571.3

NCBI Official Full Name

NEDD4 family-interacting protein 1

NCBI Official Synonym Full Names

Nedd4 family interacting protein 1

NCBI Official Symbol

NDFIP1

NCBI Official Synonym Symbols

N4WBP5

UniProt Protein Name

NEDD4 family-interacting protein 1

UniProt Synonym Protein Names

Breast cancer-associated protein SGA-1M; NEDD4 WW domain-binding protein 5; Putative MAPK-activating protein PM13; Putative NF-kappa-B-activating protein 164; Putative NFKB and MAPK-activating protein

Protein Family

NEDD4 family-interacting protein

UniProt Gene Name

NDFIP1

UniProt Synonym Gene Names

N4WBP5

UniProt Entry Name

NFIP1_HUMAN

NCBI Protein Information

NEDD4 family-interacting protein 1; Nedd4 WW domain-binding protein 5; putative MAPK-activating protein PM13; breast cancer-associated protein SGA-1M; putative NFKB and MAPK-activating protein; putative NF-kappa-B-activating protein 164

UniProt Comments for NDFIP1

NDFIP1: Activates HECT domain-containing E3 ubiquitin-protein ligases, including NEDD4 and ITCH, and consequently modulates the stability of their targets. As a result, controls many cellular processes. Prevents chronic T-helper cells-mediated inflammation by activating ITCH and thus controlling JUNB degradation. In cortical neurons, mediates the ubiquitination of SLC11A2/DMT1 by NEDD4L, leading to down-regulation of the divalent metal transporter and protection of the cells from cobalt and iron toxicity. Modulates EGFR signaling through multiple pathways. In particular, may regulate the ratio of AKT1-to-MAPK8 signaling in response to EGF, acting on AKT1 probably through PTEN destabilization and on MAPK8 through ITCH-dependent MAP2K4 inactivation. As a result, may control cell growth rate. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 5q31.3

Cellular Component: Golgi membrane; perinuclear region of cytoplasm; extracellular region; integral to membrane; endosome membrane; cell cortex

Molecular Function: signal transducer activity; protein binding; WW domain binding

Biological Process: negative regulation of interleukin-4 production; positive regulation of I-kappaB kinase/NF-kappaB cascade; ubiquitin-dependent protein catabolic process via the multivesicular body pathway; cellular iron ion homeostasis; regulation of lymphocyte differentiation; regulation of myeloid leukocyte differentiation; signal transduction; negative regulation of isotype switching to IgE isotypes; negative regulation of T cell proliferation; negative regulation of T-helper 2 type immune response; positive regulation of protein ubiquitination; negative regulation of inflammatory response; positive regulation of protein catabolic process; negative regulation of protein transport; regulation of isotype switching to IgG isotypes; negative regulation of transporter activity

Research Articles on NDFIP1

1. Ndfip1 negatively regulates RIG-I-dependent immune signaling by enhancing E3 ligase Smurf1-mediated MAVS degradation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NDFIP1 antibody

Reference Product	PubMed Publications
NEDD4 antibody	>23 publications with NDFIP1 and NEDD4

Diseases associated with anti-NDFIP1 antibody

Disease Name	Pubmed Publications
Brain Diseases Antibodies	>3 publications with NDFIP1 and Brain Diseases
Immune System Diseases Antibodies	>2 publications with NDFIP1 and Immune System Diseases
Drug Toxicity Antibodies	>1 publications with NDFIP1 and Drug Toxicity
Anemia Antibodies	>1 publications with NDFIP1 and Anemia
Drug Hypersensitivity Antibodies	>1 publications with NDFIP1 and Drug Hypersensitivity
Weight Gain Antibodies	>1 publications with NDFIP1 and Weight Gain
Hypertrophy Antibodies	>1 publications with NDFIP1 and Hypertrophy

Organs/Tissues associated with anti-NDFIP1 antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>8 publications with NDFIP1 and Brain
Blood Antibodies	>7 publications with NDFIP1 and Blood
Nerve Antibodies	>3 publications with NDFIP1 and Nerve
Skin Antibodies	>2 publications with NDFIP1 and Skin
Intestine Antibodies	>2 publications with NDFIP1 and Intestine
Lung Antibodies	>2 publications with NDFIP1 and Lung
Muscle Antibodies	>1 publications with NDFIP1 and Muscle
Spleen Antibodies	>1 publications with NDFIP1 and Spleen
Liver Antibodies	>1 publications with NDFIP1 and Liver
Embryonic Tissue Antibodies	>1 publications with NDFIP1 and Embryonic Tissue