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NDST1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6324292

NDST1 is an essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA dissacharide repeating sugar backbone to make N-sulfated… More details

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Testing Data View PDF
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Product Overview

Online Datasheet in PDF
Product Name
NDST1, Polyclonal Antibody
Full Product Name
NDST1, CT (NDST1, HSST, HSST1, Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1, Glucosaminyl N-deacetylase/N-sulfotransferase 1, N-heparan sulfate sulfotransferase 1, [Heparan sulfate]-glucosamine N-sulfotransferase 1, Heparan sulfate N-de
Product Gene Name
anti-NDST1 antibody
Product Synonym Names
NDST1; HSST; HSST1; Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1; Glucosaminyl N-deacetylase/N-sulfotransferase 1; N-heparan sulfate sulfotransferase 1; [Heparan sulfate]-glucosamine N-sulfotransferase 1; Heparan sulfate N-deacetylase 1; Heparan sulfate N-sulfotransferase 1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.
Purity/Purification
Purified by Protein A and Peptide Affinity Chromatography.
Specificity
Recognizes human NDST1.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
62,065 Da
OMIM
U18918 mRNA
3D Structure
ModBase 3D Structure for P52848
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 820-849 in the C-terminal region of human NDST1, UniProt Accession #P52848. Species sequence homology: mouse and rat
Conjugate
MaxLight405
Note
Preservative Free

Product Categories/Family for anti-NDST1 antibody

Antibodies; Enzymes; Sulfotransferase
Applications Tested/Suitable for anti-NDST1 antibody
Western Blot (WB), Immunohistochemistry (IHC), FLISA
Application Notes for anti-NDST1 antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-NDST1 antibody

Western Blot analysis in CEM cell line lysates (35ug/lane) usingMBS648643. This demonstrates that MBS648643 detected the NDST1 protein (arrow).
Rabbit Anti-NDST1 Polyclonal Antibody – Cat# MBS6324292

Immunohistochemistry (IHC) of anti-NDST1 antibody

Immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue using MBS648643 followed by peroxidase conjugation of the secondary antibody and DAB staining.
Rabbit Anti-NDST1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6324292

Product References and Citations for anti-NDST1 antibody

1. Yoshida, T., et al. Int. J. Mol. Med. 25(4):649-656(2010). 2. Oguri, M., et al. Am. J. Hypertens. 23(1):70-77(2010). 3. Duelli, A., et al. J. Immunol. 183(11):7073-7083(2009). 4. Zuberi, R.I., et al. J. Immunol. 183(6):3971-3979(2009). 5. Drenos, F., et al. Hum. Mol. Genet. 18(12):2305-2316(2009).

Other Notes

Small volumes of anti-NDST1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NDST1 purchase

MBS6324292 is an antibody targeting NDST1. Also known as NDST1, CT (NDST1, HSST, HSST1, Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1, Glucosaminyl N-deacetylase/N-sulfotransferase 1, N-heparan sulfate sulfotransferase 1, [Heparan sulfate]-glucosamine N-sulfotransferase 1, Heparan sulfate N-de. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for NDST1. It may not necessarily be applicable to this product.

NCBI GI #
4505351
NCBI GeneID
3340
NCBI Accession #
NP_001534.1
UniProt Primary Accession #
P52848
UniProt Secondary Accession #
Q96E57
UniProt Related Accession #
P52848
GenBank Nucleotide #
NM_001543.4
NCBI Official Full Name
bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1 isoform 1
NCBI Official Synonym Full Names
N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 1
NCBI Official Symbol
NDST1
NCBI Official Synonym Symbols
HSST; NST1
UniProt Protein Name
Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1
UniProt Synonym Protein Names
Glucosaminyl N-deacetylase/N-sulfotransferase 1; NDST-1; N-heparan sulfate sulfotransferase 1; N-HSST 1; [Heparan sulfate]-glucosamine N-sulfotransferase 1; HSNST 1
UniProt Gene Name
NDST1
UniProt Synonym Gene Names
HSST; HSST1; NDST-1; N-HSST 1; HSNST 1
NCBI Protein Information
bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1; NDST-1; HSNST 1; N-HSST 1; N-heparan sulfate sulfotransferase 1; glucosaminyl N-deacetylase/N-sulfotransferase 1; heparan sulfate-N-deacetylase/N-sulfotransferase; [Heparan sulfate]-glucosam

NCBI Summary for NDST1

This gene encodes a member of the heparan sulfate/heparin GlcNAc N-deacetylase/ N-sulfotransferase family. The encoded enzyme is a type II transmembrane protein that resides in the Golgi apparatus. The encoded protein catalyzes the transfer of sulfate from 3'-phosphoadenosine 5'-phosphosulfate to nitrogen of glucosamine in heparan sulfate. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]
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UniProt Comments for NDST1

Essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis (PubMed:10758005, PubMed:12634318). Plays a role in determining the extent and pattern of sulfation of heparan sulfate. Compared to other NDST enzymes, its presence is absolutely required. Participates in biosynthesis of heparan sulfate that can ultimately serve as L-selectin ligands, thereby playing a role in inflammatory response (PubMed:10758005, PubMed:12634318). Required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413).
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Research Articles on NDST1

1. These findings establish NDST1 as a target of miR-24 and demonstrate how such NDST1 suppression in endothelial cells results in reduced responsiveness to VEGFA

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NDST1 antibody

 Reference Product  PubMed Publications
 EXT1 antibody  >6 publications with NDST1 and EXT1
 HS6ST1 antibody  >4 publications with NDST1 and HS6ST1
 GPC1 antibody  >3 publications with NDST1 and GPC1
 EXT2 antibody  >3 publications with NDST1 and EXT2
 SDC1 antibody  >2 publications with NDST1 and SDC1
 SDC2 antibody  >2 publications with NDST1 and SDC2
 SDC3 antibody  >1 publications with NDST1 and SDC3
 HS6ST3 antibody  >1 publications with NDST1 and HS6ST3
 GLCE antibody  >1 publications with NDST1 and GLCE

Pathways associated with anti-NDST1 antibody

 Products by Pathway  Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Glycogen Storage Diseases Pathway antibodies  Glycogen Storage Diseases Pathway Diagram
 Glycosaminoglycan Biosynthesis - Heparan Sulfate / Heparin Pathway antibodies  Glycosaminoglycan Biosynthesis - Heparan Sulfate / Heparin Pathway Diagram
 Glycosaminoglycan Biosynthesis - Heparan Sulfate / Heparin Pathway antibodies  Glycosaminoglycan Biosynthesis - Heparan Sulfate / Heparin Pathway Diagram
 Glycosaminoglycan Biosynthesis, Heparan Sulfate Backbone Pathway antibodies  Glycosaminoglycan Biosynthesis, Heparan Sulfate Backbone Pathway Diagram
 Glycosaminoglycan Biosynthesis, Heparan Sulfate Backbone Pathway antibodies  Glycosaminoglycan Biosynthesis, Heparan Sulfate Backbone Pathway Diagram
 Glycosaminoglycan Metabolism Pathway antibodies  Glycosaminoglycan Metabolism Pathway Diagram
 HS-GAG Biosynthesis Pathway antibodies  HS-GAG Biosynthesis Pathway Diagram
 Heparan Sulfate/heparin (HS-GAG) Metabolism Pathway antibodies  Heparan Sulfate/heparin (HS-GAG) Metabolism Pathway Diagram
 MPS I - Hurler Syndrome Pathway antibodies  MPS I - Hurler Syndrome Pathway Diagram

Diseases associated with anti-NDST1 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >5 publications with NDST1 and Inflammation
 Hyperplasia Antibodies  >2 publications with NDST1 and Hyperplasia
 Disease Models, Animal Antibodies  >2 publications with NDST1 and Disease Models, Animal
 Proteinuria Antibodies  >1 publications with NDST1 and Proteinuria
 Nervous System Diseases Antibodies  >1 publications with NDST1 and Nervous System Diseases
 Fibrosis Antibodies  >1 publications with NDST1 and Fibrosis
 Kidney Diseases Antibodies  >1 publications with NDST1 and Kidney Diseases
 Hypertrophy Antibodies  >1 publications with NDST1 and Hypertrophy
 Intellectual Disability Antibodies  >1 publications with NDST1 and Intellectual Disability
 Albuminuria Antibodies  >1 publications with NDST1 and Albuminuria

Organs/Tissues associated with anti-NDST1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >10 publications with NDST1 and Blood
 Eye Antibodies  >7 publications with NDST1 and Eye
 Muscle Antibodies  >6 publications with NDST1 and Muscle
 Lung Antibodies  >6 publications with NDST1 and Lung
 Kidney Antibodies  >6 publications with NDST1 and Kidney
 Bone Antibodies  >6 publications with NDST1 and Bone
 Liver Antibodies  >5 publications with NDST1 and Liver
 Embryonic Tissue Antibodies  >5 publications with NDST1 and Embryonic Tissue
 Brain Antibodies  >4 publications with NDST1 and Brain
 Mammary Gland Antibodies  >3 publications with NDST1 and Mammary Gland
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