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NEDD8 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6489559

MBS6489559 is an antibody targeting NEDD8. Also known as NEDD8, NT (Ubiquitin-like Protein NEDD8, Neddylin, Neural Precursor Cell Expressed Developmentally Down-regulated Protein 8, NEDD-8) (MaxLight 750). Reactive in Human. Host: Rabbit. More details

$1025 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
NEDD8, Polyclonal Antibody
Full Product Name
NEDD8, NT (Ubiquitin-like Protein NEDD8, Neddylin, Neural Precursor Cell Expressed Developmentally Down-regulated Protein 8, NEDD-8) (MaxLight 750)
Product Gene Name
anti-NEDD8 antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.
Purity/Purification
Purified by Protein G Affinity Chromatography.
Specificity
Recognizes human NEDD8.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
12.8 kDa (113 aa), confirmed by MALDI-TOF.
OMIM
603171
3D Structure
ModBase 3D Structure for Q15843
Immunogen
Synthetic peptide selected from the N-terminal region of human NEDD8 (KLH).
Conjugate
MaxLight750
Note
Preservative Free

Product Categories/Family for anti-NEDD8 antibody

Antibodies; Ubiquitin
Applications Tested/Suitable for anti-NEDD8 antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes for anti-NEDD8 antibody
IHC: 1:50-1:100
FLISA: 1:1,000
WB: 1:100-1:500
Applications are based on unconjugated antibody.

Product References and Citations for anti-NEDD8 antibody

1. Wu, K., et al., J. Biol. Chem. 278(31):28882-28891 (2003). 2. Bohnsack, R.N., et al., J. Biol. Chem. 278(29):26823-26830 (2003). 3. Fan, M., et al., Mol. Endocrinol. 17(3):356-365 (2003). 4. Walden, H., et al., Nature 422(6929):330-334 (2003). 5. Liu, J., et al., Mol. Cell 10(6):1511-1518 (2002).

Other Notes

Small volumes of anti-NEDD8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NEDD8. It may not necessarily be applicable to this product.

NCBI GI #
5453760
NCBI GeneID
4738
NCBI Accession #
NP_006147
UniProt Primary Accession #
Q15843
UniProt Secondary Accession #
Q3SXN8; Q6LES6
UniProt Related Accession #
Q15843
GenBank Nucleotide #
NM_006156.2
NCBI Official Full Name
NEDD8
NCBI Official Synonym Full Names
neural precursor cell expressed, developmentally down-regulated 8
NCBI Official Symbol
NEDD8
NCBI Official Synonym Symbols
NEDD-8
UniProt Protein Name
NEDD8
UniProt Synonym Protein Names
Neddylin; Neural precursor cell expressed developmentally down-regulated protein 8; NEDD-8; Ubiquitin-like protein Nedd8
Protein Family
NEDD8
UniProt Gene Name
NEDD8
UniProt Synonym Gene Names
NEDD-8
UniProt Entry Name
NEDD8_HUMAN
NCBI Protein Information
NEDD8

UniProt Comments for NEDD8

NEDD8: a member of the ubiquitin family of proteins. Approximately 60% identical to ubiquitin protein. Activated by an E1-like complex, consisting of App-b1 and Uba3 and then linked to the E2-like enzyme, Ubc12. The major target protein modified by nedd8 is cullin-4a. Down-regulated during the development of brain.

Protein type: Ubiquitin-like modifier

Chromosomal Location of Human Ortholog: 14q12

Cellular Component: cytosol; nucleus

Molecular Function: protein binding; ubiquitin protein ligase binding

Biological Process: regulation of transcription from RNA polymerase II promoter; ubiquitin-dependent protein catabolic process; anatomical structure morphogenesis; protein localization; protein neddylation; transforming growth factor beta receptor signaling pathway; protein modification process; proteolysis; response to organic cyclic substance
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Research Articles on NEDD8

1. Furthermore, the authors characterized SENP8/DEN1 as the protease that counteracts Ubc12 auto-neddylation, and observed aberrant neddylation of Ubc12 and other NEDD8 conjugation pathway components in SENP8-deficient cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NEDD8 antibody

 Reference Product  PubMed Publications
 CUL1 antibody  >57 publications with NEDD8 and CUL1
 UBA3 antibody  >31 publications with NEDD8 and UBA3
 NUB1 antibody  >23 publications with NEDD8 and NUB1
 CUL2 antibody  >21 publications with NEDD8 and CUL2
 CUL3 antibody  >19 publications with NEDD8 and CUL3
 SENP8 antibody  >16 publications with NEDD8 and SENP8
 CDC34 antibody  >7 publications with NEDD8 and CDC34
 TP53 antibody  >6 publications with NEDD8 and TP53
 CUL4B antibody  >5 publications with NEDD8 and CUL4B
 UBA2 antibody  >5 publications with NEDD8 and UBA2

Pathways associated with anti-NEDD8 antibody

 Products by Pathway  Pathway Diagram
 Cargo Recognition For Clathrin-mediated Endocytosis Pathway antibodies  Cargo Recognition For Clathrin-mediated Endocytosis Pathway Diagram
 Clathrin-mediated Endocytosis Pathway antibodies  Clathrin-mediated Endocytosis Pathway Diagram
 Deubiquitination Pathway antibodies  Deubiquitination Pathway Diagram
 EGF/EGFR Signaling Pathway antibodies  EGF/EGFR Signaling Pathway Diagram
 Membrane Trafficking Pathway antibodies  Membrane Trafficking Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Post-translational Protein Modification Pathway antibodies  Post-translational Protein Modification Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
 Signaling By TGF-beta Receptor Complex Pathway antibodies  Signaling By TGF-beta Receptor Complex Pathway Diagram
 TGF-beta Receptor Signaling Activates SMADs Pathway antibodies  TGF-beta Receptor Signaling Activates SMADs Pathway Diagram

Diseases associated with anti-NEDD8 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >50 publications with NEDD8 and Neoplasms
 Nervous System Diseases Antibodies  >19 publications with NEDD8 and Nervous System Diseases
 Carcinoma Antibodies  >16 publications with NEDD8 and Carcinoma
 Adenocarcinoma Antibodies  >9 publications with NEDD8 and Adenocarcinoma
 Immune System Diseases Antibodies  >8 publications with NEDD8 and Immune System Diseases
 Kidney Diseases Antibodies  >7 publications with NEDD8 and Kidney Diseases
 Necrosis Antibodies  >7 publications with NEDD8 and Necrosis
 Inflammation Antibodies  >6 publications with NEDD8 and Inflammation
 Liver Diseases Antibodies  >5 publications with NEDD8 and Liver Diseases
 Cell Transformation, Neoplastic Antibodies  >4 publications with NEDD8 and Cell Transformation, Neoplastic

Organs/Tissues associated with anti-NEDD8 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >23 publications with NEDD8 and Brain
 Kidney Antibodies  >15 publications with NEDD8 and Kidney
 Blood Antibodies  >14 publications with NEDD8 and Blood
 Nerve Antibodies  >10 publications with NEDD8 and Nerve
 Vascular Antibodies  >9 publications with NEDD8 and Vascular
 Muscle Antibodies  >8 publications with NEDD8 and Muscle
 Heart Antibodies  >7 publications with NEDD8 and Heart
 Lung Antibodies  >7 publications with NEDD8 and Lung
 Testis Antibodies  >6 publications with NEDD8 and Testis
 Eye Antibodies  >5 publications with NEDD8 and Eye
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