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NEK3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS7046342

Protein kinase which influences neuronal morphogenesis and polarity through effects on microtubules. Regulates microtubule acetylation in neurons. Contributes to prolactin-mediated phosphorylation of PXN and VAV2. Implicated in prolactin-mediated cytoskeletal… More details

$230 / 0.05 mL +1 FREE 8GB USB
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
NEK3, Polyclonal Antibody
Full Product Name
NEK3 Antibody
Product Gene Name
Product Synonym Names
Serine/threonine-protein kinase Nek3; HSPK 36; Never in mitosis A-related kinase 3; NimA-related protein kinase 3; NEK3
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid
Purity/Purification
Antigen Affinity Purified
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
Molecular Weight
55,924 Da
OMIM
604044
3D Structure
ModBase 3D Structure for P51956
Species
Human
Immunogen
Recombinant human Serine/threonine-protein kinase Nek3 protein (C-240AA)
Conjugate
Non-conjugated
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-7441 / sc-292542
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-NEK3 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

Western Blot (WB) of anti-NEK3 antibody

Western blot
All lanes:  NEK3antibody at 6.04ug/ml
Lane 1 : MCF7 whole cell lysate
Lane 2 : Hela whole cell lysate
Secondary
Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 58,56 kDa
Observed band size: 58 kDa
Rabbit Anti-NEK3 Polyclonal Antibody – Cat# MBS7046342

Immunohistochemistry (IHC) of anti-NEK3 antibody

Immunohistochemistry of paraffin-embedded human colon cancer using MBS7046342 at dilution 1:100
Rabbit Anti-NEK3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS7046342

Immunohistochemistry (IHC) of anti-NEK3 antibody

Immunohistochemistry of paraffin-embedded human kidney tissue using MBS7046342 at dilution 1:100
Rabbit Anti-NEK3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS7046342

Other Notes

Small volumes of anti-NEK3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NEK3 purchase

MBS7046342 is an antibody targeting NEK3. Also known as NEK3 Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for NEK3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
serine/threonine-protein kinase Nek3 isoform b
NCBI Official Synonym Full Names
NIMA related kinase 3
NCBI Official Symbol
NEK3
NCBI Official Synonym Symbols
HSPK36
UniProt Protein Name
Serine/threonine-protein kinase Nek3
UniProt Synonym Protein Names
HSPK 36; Never in mitosis A-related kinase 3; NimA-related protein kinase 3
UniProt Gene Name
NEK3
UniProt Synonym Gene Names
NimA-related protein kinase 3
UniProt Entry Name
NEK3_HUMAN
NCBI Protein Information
serine/threonine-protein kinase Nek3

NCBI Summary for NEK3

This gene encodes a member of the NimA (never in mitosis A) family of serine/threonine protein kinases. The encoded protein differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm. The kinase is activated by prolactin stimulation, leading to phosphorylation of VAV2 guanine nucleotide exchange factor, paxillin, and activation of the RAC1 GTPase. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2009]
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UniProt Comments for NEK3

NEK3: a protein kinase of the NEK family. May play a role in mitotic regulation.

Protein type: Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; Other group; NEK family

Chromosomal Location of Human Ortholog: 13q14.13

Cellular Component: centrosome; cytoplasm; nucleus

Molecular Function: protein binding

Biological Process: chromosome segregation; microtubule-based process; protein amino acid phosphorylation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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