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NEK9 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS241459

NEK9, a NEK type protein kinase, regulates chromosome alignment and segregation in mitosis. The protein has a N-terminal NIMA-like catalytic domain, a central domain with homology to the guanine nucleotide exchange factor for the GTPase Ran (RCC1), and a… More details

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Product Overview

Product Name
NEK9, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal (IgG) to Human NEK9
Product Gene Name
Product Synonym Names
Anti-NEK9 Antibody IHC-plus; NEK9; KIAA1995; NERCC; NERCC1; Nercc1 kinase; NIMA-related kinase 8; NIMA-related kinase Nek8; NIMA-related kinase 9; NimA-related protein kinase 9; Human NEK9
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Phosphate-buffered solution, pH 7.2, 0.09% sodium azide, 50% glycerol.
Purity/Purification
Immunoaffinity Purified
Specificity
Partial recombinant protein, responding to the middle region of NEK-9
Preparation and Storage
+4 degree C or -20 degree C, Avoid repeated freezing and thawing.
Molecular Weight
107,168 Da
OMIM
609798
3D Structure
ModBase 3D Structure for Q8TD19
Target Species
Human
Immunogen Description
Recombinant human NEK9.
Immunogen Type
Recombinant protein
Immunogen
NEK9 antibody was raised against recombinant human NEK9.
Applications Tested/Suitable for anti-NEK9 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), Immunoprecipitation (IP)
Application Notes for anti-NEK9 antibody
IHC-P (1:50), IP, WB
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...

Immunohistochemistry (IHC) of anti-NEK9 antibody

Anti-NEK9 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
Rabbit Anti-NEK9 Polyclonal Antibody – Cat# MBS241459

Western Blot (WB) of anti-NEK9 antibody

Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-NEK9 polyclonal antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
Rabbit Anti-NEK9 Polyclonal Antibody, Western Blot (WB) – Cat# MBS241459

Other Notes

Small volumes of anti-NEK9 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NEK9 purchase

MBS241459 is an antibody targeting NEK9. Also known as Rabbit Polyclonal (IgG) to Human NEK9. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for NEK9. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
serine/threonine-protein kinase Nek9
NCBI Official Synonym Full Names
NIMA-related kinase 9
NCBI Official Symbol
NEK9
NCBI Official Synonym Symbols
Nek8; NERCC; NERCC1
UniProt Protein Name
Serine/threonine-protein kinase Nek9
UniProt Synonym Protein Names
Nercc1 kinase; Never in mitosis A-related kinase 9; NimA-related protein kinase 9; NimA-related kinase 8; Nek8
UniProt Gene Name
NEK9
UniProt Synonym Gene Names
KIAA1995; NEK8; NERCC; NimA-related protein kinase 9; Nek8
UniProt Entry Name
NEK9_HUMAN
NCBI Protein Information
serine/threonine-protein kinase Nek9; nercc1 kinase; nimA-related kinase 8; NIMA-related kinase Nek8; nimA-related protein kinase 9; never in mitosis A-related kinase 9; NIMA (never in mitosis gene a)- related kinase 9

UniProt Comments for NEK9

NEK9: a protein kinase related to the NIMA (never in mitosis, gene A) serine/threonine kinase of Aspergillus nidulans. Pleiotropic regulator of mitotic progression, participating in the control of spindle dynamics and chromosome separation.

Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Autophagy; Protein kinase, Other; EC 2.7.11.1; Other group; NEK family

Chromosomal Location of Human Ortholog: 14q24.3

Cellular Component: cytosol; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; metal ion binding; protein kinase binding; ATP binding

Biological Process: mitosis; cell division; mitotic nuclear envelope disassembly; mitotic cell cycle; protein amino acid phosphorylation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with anti-NEK9 antibody

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