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NEU2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6324763

MBS6324763 is an antibody targeting NEU2. Also known as NEU2, NT (NEU2, Sialidase-2, Cytosolic sialidase, N-acetyl-alpha-neuraminidase 2) (APC). Reactive in Human. Host: Rabbit. More details

$1120 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
NEU2, Polyclonal Antibody
Full Product Name
NEU2, NT (NEU2, Sialidase-2, Cytosolic sialidase, N-acetyl-alpha-neuraminidase 2) (APC)
Product Gene Name
anti-NEU2 antibody
Product Synonym Names
NEU2; Sialidase-2; Cytosolic sialidase; N-acetyl-alpha-neuraminidase 2
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Purity/Purification
Purified by Protein A and Peptide Affinity Chromatography.
Specificity
Recognizes human NEU2.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
42,254 Da
3D Structure
ModBase 3D Structure for Q9Y3R4
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 23-50 in the N-terminal region of human NEU2.
Conjugate
APC
Note
Preservative Free
Special Handling
Light sensitive

Product Categories/Family for anti-NEU2 antibody

Antibodies; Enzymes; Glycosidase
Applications Tested/Suitable for anti-NEU2 antibody
Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Immunofluorescence (IF), Flow Cytometry (FC/FACS), FLISA
Application Notes for anti-NEU2 antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-NEU2 antibody

Western Blot analysis in A549 cell line lysates (35ug/lane) usingMBS6003454. This demonstrates that MBS6003454 detected the NEU2 protein (arrow).
Rabbit Anti-NEU2 Polyclonal Antibody – Cat# MBS6324763

Immunohistochemistry (IHC) of anti-NEU2 antibody

Immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue using MBS6003454 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6003454 for immunohistochemistry.
Rabbit Anti-NEU2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6324763

Immunofluorescence (IF) of anti-NEU2 antibody

Confocal immunofluorescent analysis with A549 cells using MBS6003454 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Rabbit Anti-NEU2 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS6324763

Flow Cytometry (FC/FACS) of anti-NEU2 antibody

Flow cytometric analysis of A549 cells using MBS6003454 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-NEU2 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6324763

Product References and Citations for anti-NEU2 antibody

1. Stoppani, E., et al. Cell Biol. Int. 33(9):1020-1025(2009). 2. Li, C.Y., et al. Cell Res. 17(4):357-362(2007). 3. Chavas, L.M., et al. J. Biol. Chem. 280(1):469-475(2005). 4. Seyrantepe, V., et al. J. Biol. Chem. 279(35):37021-37029(2004). 5. Tringali, C., et al. J. Biol. Chem. 279(5):3169-3179(2004).

Other Notes

Small volumes of anti-NEU2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NEU2. It may not necessarily be applicable to this product.

NCBI GI #
222352170
NCBI GeneID
4759
NCBI Accession #
NP_005374.2
UniProt Primary Accession #
Q9Y3R4
UniProt Secondary Accession #
Q3KNW4; Q6NTB4
UniProt Related Accession #
Q9Y3R4
GenBank Nucleotide #
NM_005383.2
NCBI Official Full Name
sialidase-2
NCBI Official Synonym Full Names
neuraminidase 2
NCBI Official Symbol
NEU2
NCBI Official Synonym Symbols
SIAL2
UniProt Protein Name
Sialidase-2
UniProt Synonym Protein Names
Cytosolic sialidase; N-acetyl-alpha-neuraminidase 2
Protein Family
Sialidase
UniProt Gene Name
NEU2
NCBI Protein Information
sialidase-2

NCBI Summary for NEU2

This gene belongs to a family of glycohydrolytic enzymes which remove sialic acid residues from glycoproteins and glycolipids. Expression studies in COS7 cells confirmed that this gene encodes a functional sialidase. Its cytosolic localization was demonstrated by cell fractionation experiments. [provided by RefSeq, Jul 2008]
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UniProt Comments for NEU2

Catalyzes the removal of sialic acid (N-acetylneuraminic acid) moities from glycoproteins, oligosaccharides and gangliosides.

Research Articles on NEU2

1. Synchrotron radiation reflectometry was used to access the transverse structure of model membranes under the action of the human sialidase NEU2, down to the Angstrom length scale

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-NEU2 antibody

 Products by Pathway  Pathway Diagram
 Glycosphingolipid Metabolism Pathway antibodies  Glycosphingolipid Metabolism Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 Other Glycan Degradation Pathway antibodies  Other Glycan Degradation Pathway Diagram
 Other Glycan Degradation Pathway antibodies  Other Glycan Degradation Pathway Diagram
 Sphingolipid Metabolism Pathway antibodies  Sphingolipid Metabolism Pathway Diagram
 Sphingolipid Metabolism Pathway antibodies  Sphingolipid Metabolism Pathway Diagram
 Sphingolipid Metabolism Pathway antibodies  Sphingolipid Metabolism Pathway Diagram

Diseases associated with anti-NEU2 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >2 publications with NEU2 and Inflammation
 Hypertrophy Antibodies  >2 publications with NEU2 and Hypertrophy
 Drug Toxicity Antibodies  >1 publications with NEU2 and Drug Toxicity
 Cardiovascular Diseases Antibodies  >1 publications with NEU2 and Cardiovascular Diseases
 Necrosis Antibodies  >1 publications with NEU2 and Necrosis
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