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NFS1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6325140

Iron-sulfur clusters are required for the function of many cellular enzymes. The proteins encoded by this gene supply inorganic sulfur to these clusters by removing the sulfur from cysteine, creating alanine in the process. This gene uses alternate in-frame… More details

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Product Overview

Online Datasheet in PDF
Product Name
NFS1, Polyclonal Antibody
Full Product Name
NFS1, ID (NFS1, NIFS, Cysteine desulfurase, mitochondrial) (Azide free) (HRP)
Product Gene Name
anti-NFS1 antibody
Product Synonym Names
NFS1; NIFS; Cysteine desulfurase, mitochondrial
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
44,362 Da
OMIM
603485
3D Structure
ModBase 3D Structure for Q9Y697
Immunogen
NFS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 120-149 amino acids from the Central region of human NFS1.
Conjugate
HRP
Note
Preservative Free

Product Categories/Family for anti-NFS1 antibody

Antibodies; Mitochondrial Proteins
Applications Tested/Suitable for anti-NFS1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-NFS1 antibody
WB: 1:100-500
ELISA: 1:1,000
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-NFS1 antibody

NFS1 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the NFS1 antibody detected the NFS1 protein (arrow).
Rabbit Anti-NFS1 Polyclonal Antibody – Cat# MBS6325140

Product References and Citations for anti-NFS1 antibody

Naamati, A., et al. J. Biol. Chem. 284(44):30200-30208(2009). Marelja, Z., et al. J. Biol. Chem. 283(37):25178-25185(2008). Wu, C., et al. Proteomics 7(11):1775-1785(2007). Lamesch, P., et al. Genomics 89(3):307-315(2007). Biederbick, A., et al. Mol. Cell. Biol. 26(15):5675-5687(2006).

Other Notes

Small volumes of anti-NFS1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NFS1 purchase

MBS6325140 is an antibody targeting NFS1. Also known as NFS1, ID (NFS1, NIFS, Cysteine desulfurase, mitochondrial) (Azide free) (HRP). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for NFS1. It may not necessarily be applicable to this product.

NCBI GI #
312222766
NCBI GeneID
9054
NCBI Accession #
NP_066923.3
UniProt Primary Accession #
Q9Y697
UniProt Secondary Accession #
Q6P0L8; Q9NTZ5; Q9Y481; B3KMA5; B4DXK9; E1P5R8; F5GYK5
UniProt Related Accession #
Q9Y697
GenBank Nucleotide #
NM_021100
NCBI Official Full Name
Homo sapiens NFS1 cysteine desulfurase (NFS1), transcript variant 1, mRNA
NCBI Official Synonym Full Names
NFS1 cysteine desulfurase
NCBI Official Symbol
NFS1
NCBI Official Synonym Symbols
IscS; NIFS; HUSSY-08
UniProt Protein Name
Cysteine desulfurase, mitochondrial
Protein Family
Cysteine desulfurase
UniProt Gene Name
NFS1
UniProt Synonym Gene Names
NIFS
UniProt Entry Name
NFS1_HUMAN
NCBI Protein Information
cysteine desulfurase, mitochondrial; NFS1 nitrogen fixation 1 homolog; nitrogen fixation 1 (S. cerevisiae, homolog); nitrogen-fixing bacteria S-like protein

UniProt Comments for NFS1

NFS1: Catalyzes the removal of elemental sulfur from cysteine to produce alanine. It supplies the inorganic sulfur for iron- sulfur (Fe-S) clusters. May be involved in the biosynthesis of molybdenum cofactor. Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily. 2 isoforms of the human protein are produced by alternative initiation.

Protein type: Mitochondrial; Cofactor and Vitamin Metabolism - thiamine; EC 2.8.1.7; Transferase

Chromosomal Location of Human Ortholog: 20q11.22

Cellular Component: nucleoplasm; intracellular membrane-bound organelle; mitochondrion; mitochondrial matrix; cytoplasm; nucleolus; nucleus; cytosol

Molecular Function: protein binding; protein homodimerization activity; cysteine desulfurase activity; pyridoxal phosphate binding

Biological Process: cysteine metabolic process; iron incorporation into metallo-sulfur cluster; vitamin metabolic process; sulfur amino acid metabolic process; protein complex assembly; Mo-molybdopterin cofactor biosynthetic process; molybdopterin cofactor biosynthetic process; water-soluble vitamin metabolic process
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Research Articles on NFS1

1. The data presented here show that the Isu1 suppressor mimics the frataxin effects on Nfs1, explaining the bypassing activity.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NFS1 antibody

 Reference Product  PubMed Publications
 ISCU antibody  >14 publications with NFS1 and ISCU
 FXN antibody  >8 publications with NFS1 and FXN
 HSCB antibody  >3 publications with NFS1 and HSCB
 MOCS3 antibody  >3 publications with NFS1 and MOCS3
 MPST antibody  >1 publications with NFS1 and MPST
 LYRM4 antibody  >1 publications with NFS1 and LYRM4

Pathways associated with anti-NFS1 antibody

 Products by Pathway  Pathway Diagram
 Defective AMN Causes Hereditary Megaloblastic Anemia 1 Pathway antibodies  Defective AMN Causes Hereditary Megaloblastic Anemia 1 Pathway Diagram
 Defective BTD Causes Biotidinase Deficiency Pathway antibodies  Defective BTD Causes Biotidinase Deficiency Pathway Diagram
 Defective CD320 Causes Methylmalonic Aciduria Pathway antibodies  Defective CD320 Causes Methylmalonic Aciduria Pathway Diagram
 Defective CUBN Causes Hereditary Megaloblastic Anemia 1 Pathway antibodies  Defective CUBN Causes Hereditary Megaloblastic Anemia 1 Pathway Diagram
 Defective GIF Causes Intrinsic Factor Deficiency Pathway antibodies  Defective GIF Causes Intrinsic Factor Deficiency Pathway Diagram
 Defective HLCS Causes Multiple Carboxylase Deficiency Pathway antibodies  Defective HLCS Causes Multiple Carboxylase Deficiency Pathway Diagram
 Defective LMBRD1 Causes Methylmalonic Aciduria And Homocystinuria Type CblF Pathway antibodies  Defective LMBRD1 Causes Methylmalonic Aciduria And Homocystinuria Type CblF Pathway Diagram
 Defective MMAA Causes Methylmalonic Aciduria Type CblA Pathway antibodies  Defective MMAA Causes Methylmalonic Aciduria Type CblA Pathway Diagram
 Defective MMAB Causes Methylmalonic Aciduria Type CblB Pathway antibodies  Defective MMAB Causes Methylmalonic Aciduria Type CblB Pathway Diagram
 Defective MMACHC Causes Methylmalonic Aciduria And Homocystinuria Type CblC Pathway antibodies  Defective MMACHC Causes Methylmalonic Aciduria And Homocystinuria Type CblC Pathway Diagram

Diseases associated with anti-NFS1 antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >9 publications with NFS1 and Nervous System Diseases
 Hypertrophy Antibodies  >1 publications with NFS1 and Hypertrophy
 Liver Diseases Antibodies  >1 publications with NFS1 and Liver Diseases

Organs/Tissues associated with anti-NFS1 antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >4 publications with NFS1 and Muscle
 Liver Antibodies  >4 publications with NFS1 and Liver
 Blood Antibodies  >2 publications with NFS1 and Blood
 Heart Antibodies  >2 publications with NFS1 and Heart
 Spleen Antibodies  >1 publications with NFS1 and Spleen
 Kidney Antibodies  >1 publications with NFS1 and Kidney
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