Rabbit Anti-NISCH Polyclonal Antibody – Cat# MBS3905580

Detection of human NISCH by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NISCH antibody MBS3905580 (lot MBS3905580-1) used for IP at 6 ug per reaction. NISCH was also immunoprecipitated by rabbit anti-NISCH antibody BL21611. For blotting immunoprecipitated NISCH, MBS3905580 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

NISCH Antibody – Rabbit Polyclonal

Name: NISCH Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS3905580
Price: 250 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3905580

Nischarin (NISCH) is a nonadrenergic imidazoline-1 receptor protein that localizes to the cytosol and anchors to the inner layer of the plasma membrane. The orthologous mouse protein has been shown to influence cytoskeletal organization and cell migration by… More details

$250 / 0.01 mg +1 FREE 8GB USB

Product Documentation

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Testing Data View PDF

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Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

NISCH, Polyclonal Antibody

Full Product Name

Rabbit anti-NISCH Antibody, Affinity Purified

Product Gene Name

anti-NISCH antibody

Product Synonym Names

I1R; IRAS; IR1; Imidazoline-1 receptor candidate protein; Imidazoline receptor antisera-selected protein; Imidazoline-1 receptor; nischarin; I1R candidate protein; I-1 receptor candidate protein; I-1; hIRAS; imidazoline receptor 1; imidazoline receptor antisera selected; Nischarin; nischarin

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human

Form/Format

Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide

Purity/Purification

Antigen Affinity Purified

Concentration

1000ug/ml (lot specific)

Preparation and Storage

Store at 2-8 degree C
Shelf Life: 1 year from date of receipt

OMIM

AF082516 mRNA

3D Structure

ModBase 3D Structure for Q9Y2I1

Immunogen

Between 1100 and 1150

Conjugation

Unconjugated

Product Categories/Family for anti-NISCH antibody

Primary Antibodies

Applications Tested/Suitable for anti-NISCH antibody

Immunoprecipitation (IP)

Application Notes for anti-NISCH antibody

IP: 6ug/mg lysate

Other Notes

Small volumes of anti-NISCH antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NISCH purchase

MBS3905580 is an antibody targeting NISCH. Also known as Rabbit anti-NISCH Antibody, Affinity Purified. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for NISCH. It may not necessarily be applicable to this product.

NCBI GeneID

11188

NCBI Accession #

NP_009115.3

UniProt Primary Accession #

Q9Y2I1

UniProt Protein Name

Nischarin

UniProt Synonym Protein Names

Imidazoline receptor 1; I-1; IR1; Imidazoline receptor antisera-selected protein; hIRAS; Imidazoline-1 receptor; I1R; Imidazoline-1 receptor candidate protein; I-1 receptor candidate protein; I1R candidate protein

Protein Family

Nischarin

UniProt Gene Name

NISCH

UniProt Synonym Gene Names

IRAS; KIAA0975; I-1; IR1; hIRAS; I1R; I-1 receptor candidate protein; I1R candidate protein

UniProt Comments for NISCH

NISCH: Acts either as the functional imidazoline-1 receptor (I1R) candidate or as a membrane-associated mediator of the I1R signaling. Binds numerous imidazoline ligands that induces initiation of cell-signaling cascades triggering to cell survival, growth and migration. Its activation by the agonist rilmenidine induces an increase in phosphorylation of mitogen-activated protein kinases MAPK1 and MAPK3 in rostral ventrolateral medulla (RVLM) neurons that exhibited rilmenidine-evoked hypotension. Blocking its activation with efaroxan abolished rilmenidine-induced mitogen-activated protein kinase phosphorylation in RVLM neurons. Acts as a modulator of Rac-regulated signal transduction pathways. Suppresses Rac1-stimulated cell migration by interacting with PAK1 and inhibiting its kinase activity. Also blocks Pak-independent Rac signaling by interacting with RAC1 and inhibiting Rac1-stimulated NF-kB response element and cyclin D1 promoter activation. Inhibits also LIMK1 kinase activity by reducing LIMK1 'Tyr-508' phosphorylation. Inhibits Rac-induced cell migration and invasion in breast and colon epithelial cells. Inhibits lamellipodia formation, when overexpressed. Plays a role in protection against apoptosis. Involved in association with IRS4 in the enhancement of insulin activation of MAPK1 and MAPK3. When overexpressed, induces a redistribution of cell surface ITGA5 integrin to intracellular endosomal structures. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Receptor, GPCR

Chromosomal Location of Human Ortholog: 3p21.1

Cellular Component: cytosol; early endosome; membrane; plasma membrane; recycling endosome

Molecular Function: identical protein binding; integrin binding; phosphatidylinositol binding; protein binding

Biological Process: actin cytoskeleton organization; apoptosis; glucose metabolic process; negative regulation of cell migration; norepinephrine secretion; Rac protein signal transduction; regulation of blood pressure; regulation of synaptic transmission, GABAergic

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.