NKX3-1 Antibody – Rabbit Polyclonal

This gene encodes a homeobox-containing transcription factor. This transcription factor functions as a negative regulator of epithelial cell growth in prostate tissue. Aberrant expression of this gene is associated with prostate tumor progression. Alternate splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jan 2012]

NKX3-1: Transcription factor, which binds preferentially the consensus sequence 5'-TAAGT[AG]-3' and can behave as a transcriptional repressor. Play an important role in normal prostate development, regulating proliferation of glandular epithelium and in the formation of ducts in prostate. Act as a tumor suppressor controlling prostate carcinogenesis, as shown by the ability to inhibit proliferation and invasion activities of PC-3 prostate cancer cells. Interacts with serum response factor (SRF). Interacts with SPDEF. Interacts with WDR77. Interacts with TOPORS which polyubiquitinates NKX3-1 and induces its proteasomal degradation. By androgens and, in the LNCAP cell line, by estrogens. Androgenic control may be lost in prostate cancer cells during tumor progression from an androgen-dependent to an androgen- independent phase. Highly expressed in the prostate and, at a lower level, in the testis. Belongs to the NK-3 homeobox family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Tumor suppressor; Transcription factor

Chromosomal Location of Human Ortholog: 8p21.2

Cellular Component: intracellular; nucleus

Molecular Function: androgen receptor activity; protein binding; estrogen receptor activity; protein self-association; sequence-specific DNA binding; histone deacetylase binding; estrogen receptor binding; caspase activator activity; transcription factor binding; protein kinase activator activity; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; salivary gland development; positive regulation of transcription, DNA-dependent; multicellular organismal development; heart development; negative regulation of insulin-like growth factor receptor signaling pathway; positive regulation of caspase activity; positive regulation of mitotic cell cycle; negative regulation of cell proliferation; regulation of transcription, DNA-dependent; positive regulation of cell proliferation; protein kinase B signaling cascade; negative regulation of mitotic cell cycle; negative regulation of epithelial cell proliferation; caspase activation; somitogenesis; pharyngeal system development; male gonad development; response to testosterone stimulus; positive regulation of phosphoinositide 3-kinase cascade; branching morphogenesis of a tube; positive regulation of protein kinase activity; positive regulation of cell division; androgen receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; steroid hormone mediated signaling; positive regulation of protein amino acid phosphorylation; negative regulation of transcription, DNA-dependent; metanephros development