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NNMT Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6325895

N-methylation is one method by which drug and other xenobiotic compounds are metabolized by the liver. This gene encodes the protein responsible for this enzymatic activity which uses S-adenosyl methionine as the methyl donor. [provided by RefSeq]. More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
NNMT, Polyclonal Antibody
Full Product Name
NNMT, ID (NNMT, Nicotinamide N-methyltransferase) (AP)
Product Gene Name
Product Synonym Names
NNMT; Nicotinamide N-methyltransferase
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
29,574 Da
OMIM
600008
3D Structure
ModBase 3D Structure for P40261
Immunogen
NNMT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 100-130 amino acids from the Central region of human NNMT.
Conjugate
AP
Note
Preservative Free
Applications Tested/Suitable for anti-NNMT antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes for anti-NNMT antibody
IHC: 1:10-50
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-NNMT antibody

NNMT Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the NNMT antibody detected the NNMT protein (arrow).
Rabbit Anti-NNMT Polyclonal Antibody – Cat# MBS6325895

Immunohistochemistry (IHC) of anti-NNMT antibody

NNMT Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NNMT Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-NNMT Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6325895

Product References and Citations for anti-NNMT antibody

Giusti, B., et al. Thromb. Haemost. 104(2):231-242(2010). Zhang, J., et al. J Zhejiang Univ Sci B 11(2):136-143(2010). Emanuelli, M., et al. Histol. Histopathol. 25(1):15-20(2010). Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010): van Driel, L.M., et al. J. Nutr. 139(12):2315-2321(2009).

Other Notes

Small volumes of anti-NNMT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NNMT purchase

MBS6325895 is an antibody targeting NNMT. Also known as NNMT, ID (NNMT, Nicotinamide N-methyltransferase) (AP). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for NNMT. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
nicotinamide N-methyltransferase
NCBI Official Synonym Full Names
nicotinamide N-methyltransferase
NCBI Official Symbol
NNMT
UniProt Protein Name
Nicotinamide N-methyltransferase
UniProt Gene Name
NNMT
UniProt Entry Name
NNMT_HUMAN
NCBI Protein Information
nicotinamide N-methyltransferase

NCBI Summary for NNMT

N-methylation is one method by which drug and other xenobiotic compounds are metabolized by the liver. This gene encodes the protein responsible for this enzymatic activity which uses S-adenosyl methionine as the methyl donor. [provided by RefSeq, Jul 2008]

UniProt Comments for NNMT

NNMT: Catalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for biotransformation of many drugs and xenobiotic compounds. Belongs to the NNMT/PNMT/TEMT family.

Protein type: Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; EC 2.1.1.1; Methyltransferase

Chromosomal Location of Human Ortholog: 11q23.1

Cellular Component: cytosol

Molecular Function: nicotinamide N-methyltransferase activity

Biological Process: response to drug; methylation; organ regeneration; xenobiotic metabolic process; response to organic nitrogen
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-NNMT antibody

 Products by Pathway  Pathway Diagram
 Biological Oxidations Pathway antibodies  Biological Oxidations Pathway Diagram
 Defective AHCY Causes Hypermethioninemia With S-adenosylhomocysteine Hydrolase Deficiency (HMAHCHD) Pathway antibodies  Defective AHCY Causes Hypermethioninemia With S-adenosylhomocysteine Hydrolase Deficiency (HMAHCHD) Pathway Diagram
 Defective GCLC Causes Hemolytic Anemia Due To Gamma-glutamylcysteine Synthetase Deficiency (HAGGSD) Pathway antibodies  Defective GCLC Causes Hemolytic Anemia Due To Gamma-glutamylcysteine Synthetase Deficiency (HAGGSD) Pathway Diagram
 Defective GGT1 Causes Glutathionuria (GLUTH) Pathway antibodies  Defective GGT1 Causes Glutathionuria (GLUTH) Pathway Diagram
 Defective GSS Causes Glutathione Synthetase Deficiency (GSS Deficiency) Pathway antibodies  Defective GSS Causes Glutathione Synthetase Deficiency (GSS Deficiency) Pathway Diagram
 Defective MAT1A Causes Methionine Adenosyltransferase Deficiency (MATD) Pathway antibodies  Defective MAT1A Causes Methionine Adenosyltransferase Deficiency (MATD) Pathway Diagram
 Defective OPLAH Causes 5-oxoprolinase Deficiency (OPLAHD) Pathway antibodies  Defective OPLAH Causes 5-oxoprolinase Deficiency (OPLAHD) Pathway Diagram
 Defective SLC35D1 Causes Schneckenbecken Dysplasia (SCHBCKD) Pathway antibodies  Defective SLC35D1 Causes Schneckenbecken Dysplasia (SCHBCKD) Pathway Diagram
 Defective TPMT Causes Thiopurine S-methyltransferase Deficiency (TPMT Deficiency) Pathway antibodies  Defective TPMT Causes Thiopurine S-methyltransferase Deficiency (TPMT Deficiency) Pathway Diagram
 Defective UGT1A1 Causes Hyperbilirubinemia Pathway antibodies  Defective UGT1A1 Causes Hyperbilirubinemia Pathway Diagram
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