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NOS2/iNOS Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS2401397

Produces nitric oxide (NO) which is a messenger molecule with diversefunctions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediatescysteine S-nitrosylation of cytoplasmic target… More details

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Product Overview

Product Name
NOS2/iNOS, Polyclonal Antibody
Full Product Name
Anti-NOS2/iNOS Antibody (aa830-860)
Product Gene Name
Product Synonym Names
Rabbit Polyclonal (IgG) to Mouse NOS2/iNOS; NOS2; Hepatocyte NOS; HEP-NOS; Inducible NOS; NOS; type II; NOS type II; Inducible NO synthase; NOS2A; INOS; NOS
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
PBS, 0.09% sodium azide
Purity/Purification
Protein A purified
Specificity
This NOS2A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 830-860 amino acids from the Central region of human NOS2A.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months.
For long term storage store at -20 degree C.
Molecular Weight
126,749 Da
OMIM
163730
3D Structure
ModBase 3D Structure for P35228
Conjugation
Unconjugated
Epitope
aa830-860
Applications Tested/Suitable for anti-NOS2/iNOS antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes for anti-NOS2/iNOS antibody
FC/FACS: 1:10-1:50
IF: 1:10-1:50
IHC-P: 1:50-1:100
WB: 1:1000

Immunohistochemistry (IHC) of anti-NOS2/iNOS antibody

Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
Rabbit Anti-NOS2/iNOS Polyclonal Antibody – Cat# MBS2401397

Immunofluorescence (IF) of anti-NOS2/iNOS antibody

Confocal immunofluorescence of NOS2A Antibody with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Rabbit Anti-NOS2/iNOS Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS2401397

Western Blot (WB) of anti-NOS2/iNOS antibody

NOS2A Antibody western blot of CEM cell line lysates (35 ug/lane). The NOS2A antibody detected the NOS2A protein (arrow).
Rabbit Anti-NOS2/iNOS Polyclonal Antibody, Western Blot (WB) – Cat# MBS2401397

Flow Cytometry (FC/FACS) of anti-NOS2/iNOS antibody

NOS2A Antibody flow cytometry of CEM cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-NOS2/iNOS Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS2401397

Other Notes

Small volumes of anti-NOS2/iNOS antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NOS2/iNOS purchase

MBS2401397 is an antibody targeting NOS2/iNOS. Also known as Anti-NOS2/iNOS Antibody (aa830-860). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for NOS2/iNOS. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
nitric oxide synthase, inducible
NCBI Official Synonym Full Names
nitric oxide synthase 2
NCBI Official Symbol
NOS2
NCBI Official Synonym Symbols
NOS; INOS; NOS2A; HEP-NOS
UniProt Protein Name
Nitric oxide synthase, inducible
UniProt Synonym Protein Names
Hepatocyte NOS; HEP-NOS; Inducible NO synthase; Inducible NOS; iNOS; NOS type II; Peptidyl-cysteine S-nitrosylase NOS2
UniProt Gene Name
NOS2
UniProt Synonym Gene Names
NOS2A; HEP-NOS; Inducible NOS; iNOS
NCBI Protein Information
nitric oxide synthase, inducible

NCBI Summary for NOS2/iNOS

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008]
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UniProt Comments for NOS2/iNOS

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body (PubMed:7531687, PubMed:7544004). In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2 (). As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH on 'Cys-247' implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM (PubMed:25417112). Involved in inflammation, enhances the synthesis of proinflammatory mediators such as IL6 and IL8 (PubMed:19688109).
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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