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NPAT Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3903121

NPAT (nuclear protein of the ataxia telangiectasia mutated locus) is required for progression through the G1 and S phases of the cell cycle and for S phase entry. It activates transcription of the histone H2A, histone H2B, histone H3 and histone H4 genes in… More details

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Product Overview

Online Datasheet in PDF
Product Name
NPAT, Polyclonal Antibody
Full Product Name
Rabbit anti-NPAT Antibody, Affinity Purified
Product Gene Name
Product Synonym Names
nuclear protein of the ataxia telangiectasia mutated locus; p220; nuclear protein, co-activator of histone transcription; nuclear protein of the ATM locus; E14/NPAT; E14; protein NPAT; nuclear protein, ataxia-telangiectasia locus; Protein NPAT; nuclear protein, coactivator of histone transcription
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
154,290 Da
OMIM
D83243 mRNA
3D Structure
ModBase 3D Structure for Q14207
Immunogen
Between 700 and 750
Conjugation
Unconjugated

Product Categories/Family for anti-NPAT antibody

Applications Tested/Suitable for anti-NPAT antibody
Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes for anti-NPAT antibody
IP: 6ug/mg lysate
IHC: 1:1000-1:5000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Immunohistochemistry (IHC) of anti-NPAT antibody

Detection of human NPAT by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-NPAT (Cat. No. MBS3903121 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Rabbit Anti-NPAT Polyclonal Antibody – Cat# MBS3903121

Immunoprecipitation (IP) of anti-NPAT antibody

Detection of human NPAT by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-NPAT antibody MBS3903121 used for IP at 3 ug/mg lysate. NPAT was also immunoprecipitated by rabbit anti-NPAT antibody MBS3903122, which recognizes a downstream epitope. For blotting immunoprecipitated NPAT, MBS3903122 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Rabbit Anti-NPAT Polyclonal Antibody, Immunoprecipitation (IP) – Cat# MBS3903121

Other Notes

Small volumes of anti-NPAT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NPAT purchase

MBS3903121 is an antibody targeting NPAT. Also known as Rabbit anti-NPAT Antibody, Affinity Purified. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for NPAT. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
protein NPAT isoform 2
NCBI Official Synonym Full Names
nuclear protein, coactivator of histone transcription
NCBI Official Symbol
NPAT
NCBI Official Synonym Symbols
E14; p220; E14/NPAT
UniProt Protein Name
Protein NPAT
UniProt Synonym Protein Names
Nuclear protein of the ataxia telangiectasia mutated locus; Nuclear protein of the ATM locus; p220
Protein Family
UniProt Gene Name
NPAT
UniProt Synonym Gene Names
CAND3; E14; Nuclear protein of the ATM locus
NCBI Protein Information
protein NPAT

UniProt Comments for NPAT

Required for progression through the G1 and S phases of the cell cycle and for S phase entry. Activates transcription of the histone H2A, histone H2B, histone H3 and histone H4 genes in conjunction with MIZF. Also positively regulates the ATM, MIZF and PRKDC promoters. Transcriptional activation may be accomplished at least in part by the recruitment of the NuA4 histone acetyltransferase (HAT) complex to target gene promoters.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NPAT antibody

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