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NPFF Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6326218

FMFRamide-related protein precursor plays a role in the regulation of heart rate and blood pressure and the modulation of morphine-induced antinociception. FMRFAL encodes a preproprotein which is cleaved to form two active peptides with similar function. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
NPFF, Polyclonal Antibody
Full Product Name
NPFF, CT (NPFF, FMRFamide-related peptides, Neuropeptide SF, Neuropeptide FF, Neuropeptide AF) (FITC)
Product Gene Name
anti-NPFF antibody
Product Synonym Names
NPFF; FMRFamide-related peptides; Neuropeptide SF; Neuropeptide FF; Neuropeptide AF
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
113
OMIM
604643
3D Structure
ModBase 3D Structure for O15130
Immunogen
NPFF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 83-113 amino acids from the C-terminal region of human NPFF.
Conjugate
FITC
Note
Preservative Free
Special Handling
Light sensitive

Product Categories/Family for anti-NPFF antibody

Antibodies; Neuroscience
Applications Tested/Suitable for anti-NPFF antibody
Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
Application Notes for anti-NPFF antibody
FC/FACS: 1:10-50
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-NPFF antibody

Western blot analysis of NPFF Antibody (C-term) in 293 cell line lysates (35ug/lane).NPFF (arrow) was detected using the purified Pab.
Rabbit Anti-NPFF Polyclonal Antibody – Cat# MBS6326218

Flow Cytometry (FC/FACS) of anti-NPFF antibody

NPFF Antibody (C-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-NPFF Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6326218

Product References and Citations for anti-NPFF antibody

Yang, H.Y., et al. Neuropeptides 42(1):1-18(2008). Roumy, M., et al. J. Biol. Chem. 282(11):8332-8342(2007). Anko, M.L., et al. J. Neurochem. 96(2):573-584(2006).

Other Notes

Small volumes of anti-NPFF antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NPFF purchase

MBS6326218 is an antibody targeting NPFF. Also known as NPFF, CT (NPFF, FMRFamide-related peptides, Neuropeptide SF, Neuropeptide FF, Neuropeptide AF) (FITC). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for NPFF. It may not necessarily be applicable to this product.

NCBI GI #
8134595
NCBI GeneID
8620
NCBI Accession #
O15130.1
UniProt Primary Accession #
O15130
UniProt Secondary Accession #
Q3SXL4
UniProt Related Accession #
O15130
NCBI Official Full Name
Pro-FMRFamide-related neuropeptide FF
NCBI Official Synonym Full Names
neuropeptide FF-amide peptide precursor
NCBI Official Symbol
NPFF
NCBI Official Synonym Symbols
FMRFAL
UniProt Protein Name
Pro-FMRFamide-related neuropeptide FF
UniProt Synonym Protein Names
FMRFamide-related peptidesCleaved into the following 3 chains:Neuropeptide SF; NPSF; Neuropeptide FF; NPFF; Neuropeptide AF; NPAF
Protein Family
Pro-FMRFamide-related neuropeptide
UniProt Gene Name
NPFF
UniProt Synonym Gene Names
NPSF; NPFF; NPAF
UniProt Entry Name
NPFF_HUMAN
NCBI Protein Information
pro-FMRFamide-related neuropeptide FF

UniProt Comments for NPFF

NPFF: Morphine modulating peptides. Have wide-ranging physiologic effects, including the modulation of morphine-induced analgesia, elevation of arterial blood pressure, and increased somatostatin secretion from the pancreas. Neuropeptide FF potentiates and sensitizes ASIC1 and ASIC3 channels. Belongs to the FARP (FMRFamide related peptide) family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 12q13.13

Cellular Component: extracellular space; dendrite; extracellular region; perikaryon; nerve terminal; vesicle

Molecular Function: neuropeptide hormone activity; G-protein-coupled receptor binding; receptor binding

Biological Process: response to drug; negative regulation of heart rate; maternal process involved in pregnancy; response to morphine; negative regulation of appetite; negative regulation of insulin secretion; vasopressin secretion; synaptic transmission; elevation of cytosolic calcium ion concentration; neuropeptide signaling pathway; spinal cord development; regulation of sensory perception of pain; regulation of excitatory postsynaptic membrane potential; positive regulation of blood pressure; acute inflammatory response to antigenic stimulus
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Research Articles on NPFF

1. reduction of NPFF release within the dorsal motor nucleus and ambiguus nucleus could serve as a possible cause of the impairment of cardiac vagal control in hypertensive patients

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NPFF antibody

 Reference Product  PubMed Publications
 QRFP antibody  >7 publications with NPFF and QRFP
 CCK antibody  >6 publications with NPFF and CCK
 NPFFR2 antibody  >4 publications with NPFF and NPFFR2
 QRFPR antibody  >2 publications with NPFF and QRFPR
 NPFFR1 antibody  >2 publications with NPFF and NPFFR1

Pathways associated with anti-NPFF antibody

 Products by Pathway  Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway antibodies  Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 G Alpha (q) Signalling Events Pathway antibodies  G Alpha (q) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 Gastrin-CREB Signalling Pathway Via PKC And MAPK antibodies  Gastrin-CREB Signalling Pathway Via PKC And MAPK Diagram
 Metabolic Disorders Of Biological Oxidation Enzymes Pathway antibodies  Metabolic Disorders Of Biological Oxidation Enzymes Pathway Diagram
 Orexin And Neuropeptides FF And QRFP Bind To Their Respective Receptors Pathway antibodies  Orexin And Neuropeptides FF And QRFP Bind To Their Respective Receptors Pathway Diagram
 Peptide Ligand-binding Receptors Pathway antibodies  Peptide Ligand-binding Receptors Pathway Diagram

Diseases associated with anti-NPFF antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >15 publications with NPFF and Inflammation
 Hemorrhage Antibodies  >3 publications with NPFF and Hemorrhage
 Kidney Diseases Antibodies  >1 publications with NPFF and Kidney Diseases
 Edema Antibodies  >1 publications with NPFF and Edema
 Drug Hypersensitivity Antibodies  >1 publications with NPFF and Drug Hypersensitivity

Organs/Tissues associated with anti-NPFF antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >159 publications with NPFF and Brain
 Heart Antibodies  >15 publications with NPFF and Heart
 Intestine Antibodies  >6 publications with NPFF and Intestine
 Kidney Antibodies  >5 publications with NPFF and Kidney
 Pancreas Antibodies  >3 publications with NPFF and Pancreas
 Eye Antibodies  >2 publications with NPFF and Eye
 Epididymis Antibodies  >1 publications with NPFF and Epididymis
 Uncharacterized Tissue Antibodies  >1 publications with NPFF and Uncharacterized Tissue
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