Rabbit Anti-NPFFR2 Polyclonal Antibody – Cat# MBS1758919

Figure 1. Western blot analysis of NPFFR2 using anti-NPFFR2 antibody (MBS1758919).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NPFFR2 antigen affinity purified polyclonal antibody (#MBS1758919) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (#MBS176460) with Tanon 5200 system. A specific band was detected for NPFFR2 at approximately 60 kDa. The expected band size for NPFFR2 is at 60 kDa.

NPFFR2 Antibody – Rabbit Polyclonal

Name: NPFFR2 Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS1758919
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1758919

The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring… More details

$210 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

NPFFR2, Polyclonal Antibody

Full Product Name

Anti-NPFFR2 Antibody Picoband

Product Gene Name

anti-NPFFR2 antibody

Product Synonym Names

Disintegrin and metalloproteinase domain-containing protein 2; Disintegrin and metalloproteinase domain-containing protein 2; ADAM 2; Cancer/testis antigen 15; CT15; Fertilin subunit beta; PH-30; PH30; PH30-beta; ADAM2; FTNB

Clonality

Polyclonal

Isotype

Rabbit IgG

Host

Rabbit

Species Reactivity

Human

Form/Format

Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purity/Purification

Immunogen affinity purified.

Specificity

Expressed specifically in spermatogenic cells in the seminiferous cells. Not detected in fetal tissues.

Preparation and Storage

At -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.

Molecular Weight

60,270 Da

OMIM

607449

3D Structure

ModBase 3D Structure for Q9Y5X5

Immunogen

E coli-derived human NPFFR2 recombinant protein (Position: L14-E521).

Subcellular Localization

Membrane, Single-pass type I membrane protein

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500ug/ml.

Cross Reactivity

No cross-reactivity with other proteins.

Protein Function

Sperm surface membrane protein that may be involved in sperm-egg plasma membrane adhesion and fusion during fertilization. Could have a role in sperm-zona binding or migration of sperm from the uterus into the oviduct. Interactions with egg membrane could be mediated via binding between its disintegrin-like domain to one or more integrins receptors on the egg. This is a non catalytic metalloprotease-like protein.

Product Categories/Family for anti-NPFFR2 antibody

Primary Antibodies; Cancer; Cell Biology; Cytoskeleton/ECM; ECM Enzymes; Extracellular Matrix; Invasion/Microenvironment; Metalloprotease; Proteolysis/Ubiquitin; Proteolytic Enzymes; Signal Transduction

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-NPFFR2 antibody

Western Blot (WB), ELISA (EIA)

Application Notes for anti-NPFFR2 antibody

WB: 0.25-0.5ug/ml, Human
ELISA: 0.1-0.5ug/ml

Product References and Citations for anti-NPFFR2 antibody

1. Bonini, J. A., Jones, K. A., Adham, N., Forray, C., Artymyshyn, R., Durkin, M. M., Smith, K. E., Tamm, J. A., Boteju, L. W., Lakhlani, P. P., Raddatz, R., Yao, W.-J., Ogozalek, K. L., Boyle, N., Kouranova, E. V., Quan, Y., Vaysse, P. J, Wetzel, J. M., Branchek, T. A., Gerald, C., Borowsky, B. Identification and characterization of two G protein-coupled receptors for neuropeptide FF. J. Biol. Chem. 275: 39324-39331, 2000.2. Cikos, S., Gregor, P., Koppel, J. Sequence and tissue distribution of a novel G-protein-coupled receptor expressed prominently in human placenta. Biochem. Biophys. Res. Commun. 256: 352-356, 1999.3. Dahlman, I., Dicker, A., Jiao, H., Kere, J., Blomqvist, L., van Harmelen, V., Hoffstedt, J., Borch-Johnsen, K., Jorgensen, T., Hansen, T., Pedersen, O., Laakso, M., Arner, P. A common haplotype in the G-protein-coupled receptor gene GPR74 is associated with leanness and increased lipolysis. Am. J. Hum. Genet. 80: 1115-1124, 2007.

Other Notes

Small volumes of anti-NPFFR2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NPFFR2 purchase

MBS1758919 is an antibody targeting NPFFR2. Also known as Anti-NPFFR2 Antibody Picoband. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for NPFFR2. It may not necessarily be applicable to this product.

NCBI GI #

221316654

NCBI GeneID

10886

NCBI Accession #

NP_004876.2

UniProt Primary Accession #

Q9Y5X5

UniProt Secondary Accession #

Q96RV1; Q9NR49

UniProt Related Accession #

Q9Y5X5

GenBank Nucleotide #

NM_004885.2

NCBI Official Full Name

neuropeptide FF receptor 2 isoform 1

NCBI Official Synonym Full Names

neuropeptide FF receptor 2

NCBI Official Symbol

NPFFR2

NCBI Official Synonym Symbols

GPR74; NPFF2; NPGPR; HLWAR77

UniProt Protein Name

Neuropeptide FF receptor 2

UniProt Synonym Protein Names

G-protein coupled receptor 74; G-protein coupled receptor HLWAR77; Neuropeptide G-protein coupled receptor

Protein Family

Neuropeptide FF receptor

UniProt Gene Name

NPFFR2

UniProt Synonym Gene Names

GPR74; NPFF2; NPGPR

UniProt Entry Name

NPFF2_HUMAN

NCBI Protein Information

neuropeptide FF receptor 2; neuropeptide FF 2; G protein-coupled receptor 74; G-protein coupled receptor 74; G-protein coupled receptor HLWAR77; neuropeptide G-protein coupled receptor

NCBI Summary for NPFFR2

This gene encodes a member of a subfamily of G-protein-coupled neuropeptide receptors. This protein is activated by the neuropeptides A-18-amide (NPAF) and F-8-amide (NPFF) and may function in pain modulation and regulation of the opioid system. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2009]

UniProt Comments for NPFFR2

NPFFR2: Receptor for NPAF (A-18-F-amide) and NPFF (F-8-F-amide) neuropeptides, also known as morphine-modulating peptides. Can also be activated by a variety of naturally occurring or synthetic FMRF-amide like ligands. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol- calcium second messenger system. Belongs to the G-protein coupled receptor 1 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Receptor, GPCR; GPCR, family 1

Chromosomal Location of Human Ortholog: 4q21

Cellular Component: integral to plasma membrane; plasma membrane; actin cytoskeleton

Molecular Function: opioid receptor binding; G-protein coupled receptor activity; neuropeptide receptor activity; peptide binding

Biological Process: regulation of adenylate cyclase activity; G-protein coupled receptor protein signaling pathway; synaptic transmission; neuropeptide signaling pathway; regulation of MAPKKK cascade; detection of abiotic stimulus; cellular response to hormone stimulus

Research Articles on NPFFR2

1. These data show that NPFF-induced heterologous desensitization of MOP receptor signaling is mediated by GRK2 and could involve transphosphorylation within the heteromeric receptor complex.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NPFFR2 antibody

Reference Product	PubMed Publications
NPFFR1 antibody	>1 publications with NPFFR2 and NPFFR1
NPFF antibody	>1 publications with NPFFR2 and NPFF

Pathways associated with anti-NPFFR2 antibody

Products by Pathway	Pathway Diagram
Class A/1 (Rhodopsin-like Receptors) Pathway antibodies	Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
G Alpha (q) Signalling Events Pathway antibodies	G Alpha (q) Signalling Events Pathway Diagram
GPCR Downstream Signaling Pathway antibodies	GPCR Downstream Signaling Pathway Diagram
GPCR Ligand Binding Pathway antibodies	GPCR Ligand Binding Pathway Diagram
GPCRs, Class A Rhodopsin-like Pathway antibodies	GPCRs, Class A Rhodopsin-like Pathway Diagram
Gastrin-CREB Signalling Pathway Via PKC And MAPK antibodies	Gastrin-CREB Signalling Pathway Via PKC And MAPK Diagram
Neuroactive Ligand-receptor Interaction Pathway antibodies	Neuroactive Ligand-receptor Interaction Pathway Diagram
Neuroactive Ligand-receptor Interaction Pathway antibodies	Neuroactive Ligand-receptor Interaction Pathway Diagram
Orexin And Neuropeptides FF And QRFP Bind To Their Respective Receptors Pathway antibodies	Orexin And Neuropeptides FF And QRFP Bind To Their Respective Receptors Pathway Diagram
Peptide Ligand-binding Receptors Pathway antibodies	Peptide Ligand-binding Receptors Pathway Diagram

Diseases associated with anti-NPFFR2 antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>1 publications with NPFFR2 and Neoplasms
Inflammation Antibodies	>1 publications with NPFFR2 and Inflammation