Rabbit Anti-NPL Polyclonal Antibody – Cat# MBS6326260

NPL Antibody (N-term) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the NPL antibody detected the NPL protein (arrow).

NPL Antibody – Rabbit Polyclonal

Name: NPL Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS6326260
Price: 1060 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6326260

N-acetylneuraminate pyruvate lyase (EC 4.1.3.3) controls the cellular concentration of sialic acid by catalyzing the conversion of sialic acid into acylmannosamines and pyruvate (Wu et al., 2005 [PubMed 16147865]). More details

$1060 / 0.2 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

NPL, Polyclonal Antibody

Full Product Name

NPL, NT (NPL, C1orf13, N-acetylneuraminate lyase, N-acetylneuraminate pyruvate-lyase, N-acetylneuraminic acid aldolase, Sialate lyase, Sialate-pyruvate lyase, Sialic acid aldolase, Sialic acid lyase) (APC)

Product Gene Name

anti-NPL antibody

Product Synonym Names

NPL; C1orf13; N-acetylneuraminate lyase; N-acetylneuraminate pyruvate-lyase; N-acetylneuraminic acid aldolase; Sialate lyase; Sialate-pyruvate lyase; Sialic acid aldolase; Sialic acid lyase

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human

Form/Format

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

Purity/Purification

Purified by Protein A Affinity Chromatography.

Preparation and Storage

Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

OMIM

611412

3D Structure

ModBase 3D Structure for Q9BXD5

Immunogen

NPL antibody is generated from rabbits immunized with a KLH conjugated synthetic between 48-77 amino acids selected from the N-terminal region of human NPL.

Conjugate

APC

Note

Preservative Free

Special Handling

Light sensitive

Product Categories/Family for anti-NPL antibody

Antibodies; KIAA; orf

Applications Tested/Suitable for anti-NPL antibody

Western Blot (WB), FLISA

Application Notes for anti-NPL antibody

WB: 1:100-500
FLISA: 1:1,000
Applications are based on unconjugated antibody.

Product References and Citations for anti-NPL antibody

Chu, H.Y., et al. J Mol Model 15(3):323-328(2009). Wu, M., et al. DNA Seq. 16(2):137-142(2005). Traving, C., et al. Eur. J. Biochem. 268(24):6473-6486(2001). Sood, R., et al. Genomics 73(2):211-222(2001).

Other Notes

Small volumes of anti-NPL antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NPL purchase

MBS6326260 is an antibody targeting NPL. Also known as NPL, NT (NPL, C1orf13, N-acetylneuraminate lyase, N-acetylneuraminate pyruvate-lyase, N-acetylneuraminic acid aldolase, Sialate lyase, Sialate-pyruvate lyase, Sialic acid aldolase, Sialic acid lyase) (APC). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for NPL. It may not necessarily be applicable to this product.

NCBI GI #

13540533

NCBI GeneID

80896

NCBI Accession #

NP_110396.1

UniProt Primary Accession #

Q9BXD5

UniProt Related Accession #

Q9BXD5

GenBank Nucleotide #

NP_110396.1

NCBI Official Full Name

N-acetylneuraminate lyase isoform 1

NCBI Official Synonym Full Names

N-acetylneuraminate pyruvate lyase

NCBI Official Symbol

NPL

NCBI Official Synonym Symbols

NAL; C112; NPL1; C1orf13

UniProt Protein Name

N-acetylneuraminate lyase

UniProt Synonym Protein Names

N-acetylneuraminate pyruvate-lyase; N-acetylneuraminic acid aldolase; Sialate lyase; Sialate-pyruvate lyase; Sialic acid aldolase; Sialic acid lyase

Protein Family

NPL4-like protein

UniProt Gene Name

NPL

UniProt Synonym Gene Names

C1orf13; NALase

UniProt Entry Name

NPL_HUMAN

NCBI Protein Information

N-acetylneuraminate lyase

NCBI Summary for NPL

This gene encodes a member of the N-acetylneuraminate lyase sub-family of (beta/alpha)(8)-barrel enzymes. N-acetylneuraminate lyases regulate cellular concentrations of N-acetyl-neuraminic acid (sialic acid) by mediating the reversible conversion of sialic acid into N-acetylmannosamine and pyruvate. A pseudogene of this gene is located on the short arm of chromosome 2. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2011]

UniProt Comments for NPL

NPL: Catalyzes the cleavage of N-acetylneuraminic acid (sialic acid) to form pyruvate and N-acetylmannosamine via a Schiff base intermediate. It prevents sialic acids from being recycled and returning to the cell surface. Belongs to the DHDPS family. NanA subfamily. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Lyase; Carbohydrate Metabolism - amino sugar and nucleotide sugar; EC 4.1.3.3

Chromosomal Location of Human Ortholog: 1q25

Cellular Component: cytosol

Molecular Function: N-acetylneuraminate lyase activity

Biological Process: N-acetylneuraminate catabolic process; cellular protein metabolic process; dolichol-linked oligosaccharide biosynthetic process; protein amino acid N-linked glycosylation via asparagine; post-translational protein modification

Research Articles on NPL

1. 3D structure model of N-acetylneuraminate lyase from human (hNAL, EC 4.1.3.3) was created and refined

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-NPL antibody

Products by Pathway	Pathway Diagram
Amino Sugar And Nucleotide Sugar Metabolism Pathway antibodies	Amino Sugar And Nucleotide Sugar Metabolism Pathway Diagram
Amino Sugar And Nucleotide Sugar Metabolism Pathway antibodies	Amino Sugar And Nucleotide Sugar Metabolism Pathway Diagram
Asparagine N-linked Glycosylation Pathway antibodies	Asparagine N-linked Glycosylation Pathway Diagram
Biosynthesis Of The N-glycan Precursor (dolichol Lipid-linked Oligosaccharide, LLO) And Transfer To A Nascent Protein Pathway antibodies	Biosynthesis Of The N-glycan Precursor (dolichol Lipid-linked Oligosaccharide, LLO) And Transfer To A Nascent Protein Pathway Diagram
Defective ALG1 Causes ALG1-CDG (CDG-1k) Pathway antibodies	Defective ALG1 Causes ALG1-CDG (CDG-1k) Pathway Diagram
Defective ALG11 Causes ALG11-CDG (CDG-1p) Pathway antibodies	Defective ALG11 Causes ALG11-CDG (CDG-1p) Pathway Diagram
Defective ALG12 Causes ALG12-CDG (CDG-1g) Pathway antibodies	Defective ALG12 Causes ALG12-CDG (CDG-1g) Pathway Diagram
Defective ALG14 Causes Congenital Myasthenic Syndrome (ALG14-CMS) Pathway antibodies	Defective ALG14 Causes Congenital Myasthenic Syndrome (ALG14-CMS) Pathway Diagram
Defective ALG2 Causes ALG2-CDG (CDG-1i) Pathway antibodies	Defective ALG2 Causes ALG2-CDG (CDG-1i) Pathway Diagram
Defective ALG3 Causes ALG3-CDG (CDG-1d) Pathway antibodies	Defective ALG3 Causes ALG3-CDG (CDG-1d) Pathway Diagram

Diseases associated with anti-NPL antibody

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>77 publications with NPL and Nervous System Diseases
Neurobehavioral Manifestations Antibodies	>12 publications with NPL and Neurobehavioral Manifestations
Kidney Diseases Antibodies	>10 publications with NPL and Kidney Diseases
Heart Diseases Antibodies	>10 publications with NPL and Heart Diseases
Breast Neoplasms Antibodies	>10 publications with NPL and Breast Neoplasms
Pain Antibodies	>8 publications with NPL and Pain
Inflammation Antibodies	>7 publications with NPL and Inflammation
Depressive Disorder Antibodies	>6 publications with NPL and Depressive Disorder
Neoplasms, Experimental Antibodies	>5 publications with NPL and Neoplasms, Experimental
Disease Models, Animal Antibodies	>5 publications with NPL and Disease Models, Animal

Organs/Tissues associated with anti-NPL antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>96 publications with NPL and Blood
Brain Antibodies	>39 publications with NPL and Brain
Eye Antibodies	>34 publications with NPL and Eye
Skin Antibodies	>24 publications with NPL and Skin
Kidney Antibodies	>20 publications with NPL and Kidney
Prostate Antibodies	>19 publications with NPL and Prostate
Heart Antibodies	>18 publications with NPL and Heart
Bone Antibodies	>15 publications with NPL and Bone
Lung Antibodies	>10 publications with NPL and Lung
Thyroid Antibodies	>8 publications with NPL and Thyroid