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NRF2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS4750305

Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Product Name
Nrf2, Polyclonal Antibody
Full Product Name
Anti-Phospho-Nrf2 (S40) Rabbit pAb
Product Gene Name
Product Synonym Gene Name
E4TF1-60; E4TF1A; NFT2; NRF2; NRF2A
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity Chromatography
Specificity
Phospho-Nrf2 (S40) Antibody detects endogenous levels of total Phospho-Nrf2 (S40)
Preparation and Storage
Store at 4 degree C for short term.
Store at-20 degree C for long term.
Avoid freeze/thaw cycle.
Molecular Weight
90kDa
OMIM
600492
3D Structure
ModBase 3D Structure for Q16236
Immunogen
A synthesized peptide derived from human Phospho-Nrf2 (S40)
Subcellular Location
Cytoplasm
Applications Tested/Suitable for anti-Nrf2 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes for anti-Nrf2 antibody
WB: 1:1000-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
IP: 1:50
FC/FACS: 1:50

Western Blot (WB) of anti-Nrf2 antibody

Western blot analysis of Nrf2 phosphorylation expression in HepG2 cell lysate.
Rabbit Anti-NRF2 Polyclonal Antibody – Cat# MBS4750305

Immunohistochemistry (IHC) of anti-Nrf2 antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-Nrf2 (S40) Antibody.
Rabbit Anti-NRF2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS4750305

Immunofluorescence (IF) of anti-Nrf2 antibody

Immunofluorescent analysis of HepG2 cells, using Phospho-Nrf2 (S40) Antibody.
Rabbit Anti-NRF2 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS4750305

Other Notes

Small volumes of anti-Nrf2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Nrf2 purchase

MBS4750305 is an antibody targeting Nrf2. Also known as Anti-Phospho-Nrf2 (S40) Rabbit pAb. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Nrf2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
nuclear factor erythroid 2-related factor 2 isoform 2
NCBI Official Synonym Full Names
nuclear factor, erythroid 2 like 2
NCBI Official Symbol
NFE2L2
NCBI Official Synonym Symbols
NRF2; HEBP1; IMDDHH
UniProt Protein Name
Nuclear factor erythroid 2-related factor 2
UniProt Synonym Protein Names
HEBP1; Nuclear factor, erythroid derived 2, like 2
UniProt Gene Name
NFE2L2
UniProt Synonym Gene Names
NRF2; NF-E2-related factor 2; NFE2-related factor 2
NCBI Protein Information
nuclear factor erythroid 2-related factor 2

NCBI Summary for Nrf2

This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. Multiple transcript variants encoding different isoforms have been characterized for this gene. [provided by RefSeq, Sep 2015]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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