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OCRL Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1758259

The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring… More details

$210 / 0.01 mg +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
OCRL, Polyclonal Antibody
Full Product Name
Anti-OCRL Antibody Picoband
Product Gene Name
anti-OCRL antibody
Product Synonym Names
G2/mitotic-specific cyclin-B2; cyclin B2; G2/mitotic-specific cyclin-B2; CCNB2
Clonality
Polyclonal
Isotype
Rabbit IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Purity/Purification
Immunogen affinity purified.
Specificity
StronglyExpressed in placenta. Expressed at lower levels in heart, pancreas, kidney and brain.Expressed in endothelial cells. Isoform alpha was found to be the predominant isoform. Isoform beta was not found in pancreas and brain.
Preparation and Storage
At -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.
Molecular Weight
103,227 Da
3D Structure
ModBase 3D Structure for Q01968
Immunogen
E coli-derived human OCRL recombinant protein (Position: M1-D901).
Subcellular Localization
Centrosome. Microtubule cytoskeleton. Cytosol. Nucleus. Cyclin-dependent protein kinase holoenzyme complex. Cytoplasm. Membrane.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500ug/ml.
Cross Reactivity
No cross-reactivity with other proteins.
Protein Function
Essential for the control of the cell cycle at the G2/M (mitosis) transition.

Product Categories/Family for anti-OCRL antibody

Primary Antibodies; Signal Transduction
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-OCRL antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA (EIA)
Application Notes for anti-OCRL antibody
WB: 0.25-0.5ug/ml, Human
IHC(Paraffin-embedded Section): 2-5ug/ml, Human
ICC/IF: 5ug/ml, Human
ELISA: 0.1-0.5ug/ml

Western Blot (WB) of anti-OCRL antibody

Figure 1. Western blot analysis of OCRL using anti-OCRL antibody (MBS1758259).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OCRL antigen affinity purified polyclonal antibody (#MBS1758259) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (#MBS176460) with Tanon 5200 system. A specific band was detected for OCRL at approximately 104 kDa. The expected band size for OCRL is at 104 kDa.
Rabbit Anti-OCRL Polyclonal Antibody – Cat# MBS1758259

Immunohistochemistry (IHC) of anti-OCRL antibody

Figure 2. IHC analysis of OCRL using anti-OCRL antibody (MBS1758259).
OCRL was detected in a paraffin-embedded section of human colon adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-OCRL Antibody (MBS1758259) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (#MBS176453) with DAB as the chromogen.
Rabbit Anti-OCRL Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS1758259

Immunohistochemistry (IHC) of anti-OCRL antibody

Figure 3. IHC analysis of OCRL using anti-OCRL antibody (MBS1758259).
OCRL was detected in a paraffin-embedded section of human endometrioid adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-OCRL Antibody (MBS1758259) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (#MBS176453) with DAB as the chromogen.
Rabbit Anti-OCRL Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS1758259

Immunohistochemistry (IHC) of anti-OCRL antibody

Figure 4. IHC analysis of OCRL using anti-OCRL antibody (MBS1758259).
OCRL was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-OCRL Antibody (MBS1758259) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (#MBS176453) with DAB as the chromogen.
Rabbit Anti-OCRL Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS1758259

Immunofluorescence (IF) of anti-OCRL antibody

Figure 5. IF analysis of OCRL using anti-OCRL antibody (MBS1758259).
OCRL was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (#MBS176582) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5ug/mL rabbit anti-OCRL Antibody (MBS1758259) overnight at 4 degree C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Rabbit Anti-OCRL Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS1758259

Product References and Citations for anti-OCRL antibody

1. Attree, O., Olivos, I. M., Okabe, I., Bailey, L. C., Nelson, D. L., Lewis, R. A., McInnes, R. R., Nussbaum, R. L. The Lowe's oculocerebrorenal syndrome gene encodes a protein highly homologous to inositol polyphosphate-5-phosphatase. Nature 358: 239-242, 1992.2. Bailey, L. C., Jr., Olivos, I. M., Leahey, A. M., Attree, O. F., Okabe, I., Lewis, R. A., MacInnes, R. R., Spinner, N. B., Nelson, D. L., Nussbaum, R. L. Characterization of a candidate gene for OCRL. (Abstract) Am. J. Hum. Genet. 51 (suppl.): A4 only, 1992.3. Bockenhauer, D., Bokenkamp, A., Nuutinen, M., Unwin, R., van't Hoff, W., Sirimanna, T., Vrljicak, K., Ludwig, M. Novel OCRL mutations in patients with Dent-2 disease. J. Pediat. Genet. 1: 15-23, 2012.

Other Notes

Small volumes of anti-OCRL antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for OCRL purchase

MBS1758259 is an antibody targeting OCRL. Also known as Anti-OCRL Antibody Picoband. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for OCRL. It may not necessarily be applicable to this product.

NCBI GI #
13325072
NCBI GeneID
4952
NCBI Accession #
NP_000267.2
UniProt Primary Accession #
Q01968
UniProt Secondary Accession #
O60800; Q15684; Q15774; Q4VY09; Q4VY10; Q5JQF1; Q5JQF2; Q9UJG5; A6NKI1; A8KAP2; B7ZLX2
UniProt Related Accession #
Q01968
GenBank Nucleotide #
NM_000276.3
NCBI Official Full Name
inositol polyphosphate 5-phosphatase OCRL-1 isoform a
NCBI Official Synonym Full Names
OCRL, inositol polyphosphate-5-phosphatase
NCBI Official Symbol
OCRL
NCBI Official Synonym Symbols
LOCR; NPHL2; OCRL1; INPP5F; OCRL-1
UniProt Protein Name
Inositol polyphosphate 5-phosphatase OCRL-1
UniProt Synonym Protein Names
Lowe oculocerebrorenal syndrome protein
Protein Family
Inositol polyphosphate 5-phosphatase
UniProt Gene Name
OCRL
UniProt Synonym Gene Names
INPP5F; OCRL1
NCBI Protein Information
inositol polyphosphate 5-phosphatase OCRL-1

NCBI Summary for OCRL

This gene encodes an inositol polyphosphate 5-phosphatase. This protein is involved in regulating membrane trafficking and is located in numerous subcellular locations including the trans-Golgi network, clathrin-coated vesicles and, endosomes and the plasma membrane. This protein may also play a role in primary cilium formation. Mutations in this gene cause oculocerebrorenal syndrome of Lowe and also Dent disease. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]
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UniProt Comments for OCRL

Converts phosphatidylinositol 4,5-bisphosphate to phosphatidylinositol 4-phosphate. Also converts inositol 1,4,5-trisphosphate to inositol 1,4-bisphosphate and inositol 1,3,4,5-tetrakisphosphate to inositol 1,3,4-trisphosphate (PubMed:25869668, PubMed:7761412, PubMed:9430698). May function in lysosomal membrane trafficking by regulating the specific pool of phosphatidylinositol 4,5-bisphosphate that is associated with lysosomes. Involved in primary cilia assembly (PubMed:22228094, PubMed:22543976).
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Research Articles on OCRL

1. loss of OCRL results in abnormal distribution of PI(4,5)P2 in the proximal regions of cilia.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-OCRL antibody

 Products by Pathway  Pathway Diagram
 1D-myo-inositol Hexakisphosphate Biosynthesis II (mammalian) Pathway antibodies  1D-myo-inositol Hexakisphosphate Biosynthesis II (mammalian) Pathway Diagram
 1D-myo-inositol Hexakisphosphate Biosynthesis II (mammalian) Pathway antibodies  1D-myo-inositol Hexakisphosphate Biosynthesis II (mammalian) Pathway Diagram
 3-phosphoinositide Degradation Pathway antibodies  3-phosphoinositide Degradation Pathway Diagram
 3-phosphoinositide Degradation Pathway antibodies  3-phosphoinositide Degradation Pathway Diagram
 Clathrin Derived Vesicle Budding Pathway antibodies  Clathrin Derived Vesicle Budding Pathway Diagram
 D-myo-inositol (1,3,4)-trisphosphate Biosynthesis Pathway antibodies  D-myo-inositol (1,3,4)-trisphosphate Biosynthesis Pathway Diagram
 D-myo-inositol (1,3,4)-trisphosphate Biosynthesis Pathway antibodies  D-myo-inositol (1,3,4)-trisphosphate Biosynthesis Pathway Diagram
 D-myo-inositol (1,4,5)-trisphosphate Degradation Pathway antibodies  D-myo-inositol (1,4,5)-trisphosphate Degradation Pathway Diagram
 D-myo-inositol (1,4,5)-trisphosphate Degradation Pathway antibodies  D-myo-inositol (1,4,5)-trisphosphate Degradation Pathway Diagram
 Golgi Associated Vesicle Biogenesis Pathway antibodies  Golgi Associated Vesicle Biogenesis Pathway Diagram

Diseases associated with anti-OCRL antibody

 Disease Name  Pubmed Publications
 Oculocerebrorenal Syndrome Antibodies  >145 publications with OCRL and Oculocerebrorenal Syndrome
 Kidney Diseases Antibodies  >134 publications with OCRL and Kidney Diseases
 Nervous System Diseases Antibodies  >124 publications with OCRL and Nervous System Diseases
 Proteinuria Antibodies  >23 publications with OCRL and Proteinuria
 Dent Disease 2 Antibodies  >8 publications with OCRL and Dent Disease 2
 Disease Models, Animal Antibodies  >6 publications with OCRL and Disease Models, Animal
 Neurobehavioral Manifestations Antibodies  >6 publications with OCRL and Neurobehavioral Manifestations
 Developmental Disabilities Antibodies  >4 publications with OCRL and Developmental Disabilities
 Dent disease 1 Antibodies  >4 publications with OCRL and Dent disease 1
 Weight Loss Antibodies  >3 publications with OCRL and Weight Loss

Organs/Tissues associated with anti-OCRL antibody

 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >47 publications with OCRL and Kidney
 Brain Antibodies  >18 publications with OCRL and Brain
 Eye Antibodies  >15 publications with OCRL and Eye
 Muscle Antibodies  >9 publications with OCRL and Muscle
 Bone Antibodies  >7 publications with OCRL and Bone
 Skin Antibodies  >6 publications with OCRL and Skin
 Intestine Antibodies  >2 publications with OCRL and Intestine
 Embryonic Tissue Antibodies  >2 publications with OCRL and Embryonic Tissue
 Liver Antibodies  >2 publications with OCRL and Liver
 Testis Antibodies  >2 publications with OCRL and Testis
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