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OGN Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6000369

This gene encodes a protein which induces ectopic bone formation in conjunction with transforming growth factor beta. This protein is a small proteoglycan which contains tandem leucine-rich repeats (LRR). Alternatively spliced transcript variants that encode… More details

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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
OGN, Polyclonal Antibody
Full Product Name
OGN, CT (OGN, OIF, SLRR3A, Mimecan, Osteoglycin, Osteoinductive factor)
Product Gene Name
Product Synonym Names
Anti -OGN, CT (OGN, OIF, SLRR3A, Mimecan, Osteoglycin, Osteoinductive factor)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
33,922 Da
Chromosome Location
Chromosome: 9; NC_000009.11 (95146249..95166976, complement). Location: 9q22
OMIM
602383
3D Structure
ModBase 3D Structure for P20774
Immunogen
OGN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 253-284 amino acids from the C-terminal region of human OGN.

Product Categories/Family for anti-OGN antibody

Applications Tested/Suitable for anti-OGN antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes for anti-OGN antibody
Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Flow Cytometry: 1:10-50

Western Blot (WB) of anti-OGN antibody

Western Blot analysis using MBS6000369 (1:500)dilution Lane 1: CCRF-CEM whole cell lysate Lane 2: MCF-7 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000.
Rabbit Anti-OGN Polyclonal Antibody – Cat# MBS6000369

Immunohistochemistry (IHC) of anti-OGN antibody

Immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue using MBS6000369 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6000369 for immunohistochemistry.
Rabbit Anti-OGN Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6000369

Flow Cytometry (FC/FACS) of anti-OGN antibody

Flow Cytometric analysis of 293 cells using MBS6000369 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-OGN Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6000369

Testing Data of anti-OGN antibody

Rabbit Anti-OGN Polyclonal Antibody, Testing Data – Cat# MBS6000369

Other Notes

Small volumes of anti-OGN antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for OGN purchase

MBS6000369 is an antibody targeting OGN. Also known as OGN, CT (OGN, OIF, SLRR3A, Mimecan, Osteoglycin, Osteoinductive factor). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for OGN. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
OGN
NCBI Official Synonym Full Names
osteoglycin
NCBI Official Symbol
OGN
NCBI Official Synonym Symbols
OG; OIF; SLRR3A
UniProt Protein Name
Mimecan
UniProt Synonym Protein Names
Osteoglycin; Osteoinductive factor
Protein Family
UniProt Gene Name
OGN
UniProt Synonym Gene Names
OIF; SLRR3A; OIF
UniProt Entry Name
MIME_HUMAN
NCBI Protein Information
mimecan; mimecan proteoglycan; osteoinductive factor; corneal keratan sulfate proteoglycan

NCBI Summary for OGN

This gene encodes a protein which induces ectopic bone formation in conjunction with transforming growth factor beta. This protein is a small proteoglycan which contains tandem leucine-rich repeats (LRR). Alternatively spliced transcript variants that encode the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for OGN

OGN: Induces bone formation in conjunction with TGF-beta-1 or TGF-beta-2. Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class III subfamily.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 9q22

Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; lysosomal lumen; Golgi lumen; extracellular region; vesicle

Molecular Function: protein binding; growth factor activity

Biological Process: keratan sulfate metabolic process; glycosaminoglycan metabolic process; negative regulation of smooth muscle cell proliferation; carbohydrate metabolic process; keratan sulfate biosynthetic process; pathogenesis; keratan sulfate catabolic process
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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