ORC1 Antibody – Rabbit Polyclonal

The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is the largest subunit of the ORC complex. While other ORC subunits are stable throughout the cell cycle, the levels of this protein vary during the cell cycle, which has been shown to be controlled by ubiquitin-mediated proteolysis after initiation of DNA replication. This protein is found to be selectively phosphorylated during mitosis. It is also reported to interact with MYST histone acetyltransferase 2 (MyST2/HBO1), a protein involved in control of transcription silencing. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]

ORC1L: Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent, however specific DNA sequences that define origins of replication have not been identified so far. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication. ORC is composed of six subunits. In human, ORC is cell cycle-dependent regulated: it is sequentially assembled at the exit from anaphase of mitosis and disassembled as cells enter S phase. Interacts with CDC6 and KAT7/HBO1. Belongs to the ORC1 family.

Protein type: DNA-binding; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 1p32

Cellular Component: nucleoplasm; cytoplasm; plasma membrane; nucleolus; nuclear origin of replication recognition complex; cytosol; nucleus; origin recognition complex

Molecular Function: protein binding; DNA binding; chromatin binding; ATP binding

Biological Process: G1/S-specific transcription in mitotic cell cycle; DNA replication initiation; mitotic cell cycle; DNA replication; G1/S transition of mitotic cell cycle

Disease: Meier-gorlin Syndrome 1