• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

p53 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9144444

This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest,… More details

$225 / 0.02 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
p53, Polyclonal Antibody
Full Product Name
[KO Validated] p53 Rabbit pAb
Product Gene Name
anti-p53 antibody
Product Synonym Names
TP53; BCC7; LFS1; P53; TRP53; tumor protein p53
Immunogen Sequence
MFVSDFRKEF YEVVQSQRVL LFVASDVDAL CACKILQALF QCDHVQYTLV PVSGWQELET AFLEHKEQFH YFILINCGAN VDLLDILQPD EDTIFFVCDT HRPVNVVNVY NDTQIKLLIK QDDDLEVPAY EDIFRDEEED EEHSGNDSDG SEPSEKRTRL EEEIVEQTMR RRQRREWEAR RRDILFDYEQ YEYHGTSSAM VMFELAWMLS KDLNDMLWWA IVGLTDQWVQ DKITQMKYVT DVGVLQRHVS RHNHRNEDEE NTLSVDCTRI SFEYDLRLVL YQHWSLHDSL CNTSYTAARF KLWSVHGQKR LQEFLADMGL PLKQVKQKFQ AMDISLKENL REMIEESANK FGMKDMRVQT FSIHFGFKHK FLASDVVFAT MSLMESPEKD GSGTDHFIQA LDSLSRSNLD KLYHGLELAK KQLRATQQTI ASCLCTNLVI SQGPFLYCSL MEGTPDVMLF SRPASLSLLS KHLLKSFVCS TKNRRCKLLP LVMAAPLSME HGTVTVVGIP PETDSSDRKN FFGRAFEKAA ESTSSRMLHN HFDLSVIELK AEDRSKFLDA LISLLS
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Purity/Purification
Affinity purification
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
Molecular Weight
566
OMIM
603465
3D Structure
ModBase 3D Structure for O75419
Positive Samples
293T
Immunogen
Recombinant protein of human p53.
Cellular Location
Cytoplasm, Endoplasmic reticulum, Mitochondrion matrix, Nucleus, PML body

Product Categories/Family for anti-p53 antibody

Epigenetics & Nuclear Signaling; Transcription Factors; DNA Damage & Repair; Cancer; Tumor suppressors; p53 pathway; Signal Transduction; PI3K-Akt Signaling Pathway; ErbB-HER Signaling Pathway; MAPK-JNK Signaling Pathway; MAPK-P38 Signaling Pathway; Cell
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-p53 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP)
Application Notes for anti-p53 antibody
WB: 1:500-1:2000
IHC: 1:50-1:200
IF/ICC: 1:50-1:200
IP: 1:50-1:200
ChIP: 1:50-1:200

Western Blot (WB) of anti-p53 antibody

Western blot analysis of extracts of 293T cells, using p53 Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Rabbit Anti-p53 Polyclonal Antibody – Cat# MBS9144444

Western Blot (WB) of anti-p53 antibody

Western blot analysis of extracts from wild type (WT) and p53 knockout (KO) 293T cells, using p53 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Rabbit Anti-p53 Polyclonal Antibody, Western Blot (WB) – Cat# MBS9144444

Immunohistochemistry (IHC) of anti-p53 antibody

Immunohistochemistry of paraffin-embedded human colon carcinoma using [KO Validated] p53 Rabbit pAb at dilution of 1:300 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Rabbit Anti-p53 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9144444

Immunofluorescence (IF) of anti-p53 antibody

Confocal immunofluorescence analysis of Hela cells using p53 Polyclonal Antibody at dilution of 1:200. Blue: DAPI for nuclear staining.
Rabbit Anti-p53 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS9144444

Immunofluorescence (IF) of anti-p53 antibody

Confocal immunofluorescence analysis of U-2 OS cells using p53 Polyclonal Antibody at dilution of 1:200. Blue: DAPI for nuclear staining.
Rabbit Anti-p53 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS9144444

Immunoprecipitation (IP) of anti-p53 antibody

Immunoprecipitation analysis of 200ug extracts of 293T cells using 3ug p53 antibody. Western blot was performed from the immunoprecipitate using p53 antibody at a dilition of 1:3000.
Rabbit Anti-p53 Polyclonal Antibody, Immunoprecipitation (IP) – Cat# MBS9144444

Chromatin Immunoprecipitation (ChIP) of anti-p53 antibody

Chromatin immunoprecipitation analysis of extracts of 293T cells, using p53 antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Rabbit Anti-p53 Polyclonal Antibody, Chromatin Immunoprecipitation (ChIP) – Cat# MBS9144444

Other Notes

Small volumes of anti-p53 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for p53 purchase

MBS9144444 is an antibody targeting p53. Also known as [KO Validated] p53 Rabbit pAb. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for p53. It may not necessarily be applicable to this product.

NCBI GI #
6685264
NCBI GeneID
8318
NCBI Accession #
O75419.1
UniProt Primary Accession #
O75419
UniProt Secondary Accession #
O60856; Q20WK8; Q6UW54; Q9UP68; B4DDB4; B4DDU3; E9PDH7
UniProt Related Accession #
O75419
NCBI Official Full Name
Cell division control protein 45 homolog
NCBI Official Synonym Full Names
cell division cycle 45
NCBI Official Symbol
CDC45
NCBI Official Synonym Symbols
CDC45L; CDC45L2; PORC-PI-1
UniProt Protein Name
Cell division control protein 45 homolog
UniProt Synonym Protein Names
PORC-PI-1
Protein Family
p53 and DNA damage-regulated protein
UniProt Gene Name
CDC45
UniProt Synonym Gene Names
CDC45L; CDC45L2
UniProt Entry Name
CDC45_HUMAN
NCBI Protein Information
cell division control protein 45 homolog; human CDC45; CDC45-related protein; cell division cycle 45-like 2; cell division cycle 45 homolog; CDC45 cell division cycle 45 homolog

NCBI Summary for p53

The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
Show more

UniProt Comments for p53

CDC45L: Required for initiation of chromosomal DNA replication. Associated with ORC2. Widely expressed, highest levels are found in adult testis and thymus and in fetal liver. Belongs to the CDC45 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 22q11.21

Cellular Component: nucleoplasm; centrosome; cytoplasm; nucleus

Molecular Function: protein binding; 3'-5' DNA helicase activity; DNA replication origin binding; chromatin binding; single-stranded DNA binding

Biological Process: G1/S-specific transcription in mitotic cell cycle; DNA replication initiation; regulation of chromatin silencing at telomere; mitotic cell cycle; DNA strand elongation during DNA replication; DNA replication checkpoint; DNA replication; DNA duplex unwinding; double-strand break repair via break-induced replication; G1/S transition of mitotic cell cycle; pre-replicative complex assembly
Show more

Research Articles on p53

1. The findings suggest that hCdc45 not only binds to but also slides on DNA with a 3'-5' polarity and, thereby acts as a molecular 'wedge' to initiate DNA strand displacement.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-p53 antibody

 Products by Pathway  Pathway Diagram
 Activation Of ATR In Response To Replication Stress Pathway antibodies  Activation Of ATR In Response To Replication Stress Pathway Diagram
 Activation Of The Pre-replicative Complex Pathway antibodies  Activation Of The Pre-replicative Complex Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Checkpoints Pathway antibodies  Cell Cycle Checkpoints Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 DNA Replication Pathway antibodies  DNA Replication Pathway Diagram
 DNA Replication Pathway antibodies  DNA Replication Pathway Diagram

Diseases associated with anti-p53 antibody

 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >8 publications with p53 and Cardiovascular Diseases
 Intestinal Neoplasms Antibodies  >2 publications with p53 and Intestinal Neoplasms
 Nervous System Diseases Antibodies  >2 publications with p53 and Nervous System Diseases
 Adenocarcinoma Antibodies  >2 publications with p53 and Adenocarcinoma
 Carcinoma, Squamous Cell Antibodies  >1 publications with p53 and Carcinoma, Squamous Cell
 Inflammation Antibodies  >1 publications with p53 and Inflammation
 Nervous System Malformations Antibodies  >1 publications with p53 and Nervous System Malformations
 Precancerous Conditions Antibodies  >1 publications with p53 and Precancerous Conditions
 Breast Neoplasms Antibodies  >1 publications with p53 and Breast Neoplasms

Organs/Tissues associated with anti-p53 antibody

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >4 publications with p53 and Lung
 Embryonic Tissue Antibodies  >4 publications with p53 and Embryonic Tissue
 Blood Antibodies  >3 publications with p53 and Blood
 Heart Antibodies  >3 publications with p53 and Heart
 Brain Antibodies  >2 publications with p53 and Brain
 Ovary Antibodies  >1 publications with p53 and Ovary
 Bone Marrow Antibodies  >1 publications with p53 and Bone Marrow
 Placenta Antibodies  >1 publications with p53 and Placenta
 Thymus Antibodies  >1 publications with p53 and Thymus
 Lymph Antibodies  >1 publications with p53 and Lymph
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000