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P5F1B Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6330322

This intronless gene was thought to be a transcribed pseudogene of POU class 5 homeobox 1, however, it has been reported that this gene can encode a functional protein. The encoded protein is nearly the same length as and highly similar to the POU class 5… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
P5F1B, Polyclonal Antibody
Full Product Name
P5F1B, CT (POU5F1B, OTF3C, OTF3P1, POU5F1P1, POU5FLC20, POU5FLC8, Putative POU domain, class 5, transcription factor 1B, Octamer-binding protein 3-like, Octamer-binding transcription factor 3-like) (Azide free) (HRP)
Product Gene Name
Product Synonym Gene Name
POU5F1B
Product Synonym Names
POU5F1B; OTF3C; OTF3P1; POU5F1P1; POU5FLC20; POU5FLC8; Putative POU domain; class 5; transcription factor 1B; Octamer-binding protein 3-like; Octamer-binding transcription factor 3-like
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
38,588 Da
OMIM
615739
3D Structure
ModBase 3D Structure for Q06416
Immunogen
P5F1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 217-247 amino acids from the C-terminal region of human P5F1B.
Conjugate
HRP
Note
Preservative Free
Applications Tested/Suitable for anti-P5F1B antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-P5F1B antibody
WB: 1:100-500
ELISA: 1:1,000
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-P5F1B antibody

P5F1B Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the P5F1B antibody detected the P5F1B protein (arrow).
Rabbit Anti-P5F1B Polyclonal Antibody – Cat# MBS6330322

Flow Cytometry (FC/FACS) of anti-P5F1B antibody

P5F1B Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-P5F1B Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6330322

Product References and Citations for anti-P5F1B antibody

Kastler, S., et al. Prostate 70(6):666-674(2010). White, K.L., et al. Twin Res Hum Genet 13(1):43-56(2010). Crowther-Swanepoel, D., et al. Nat. Genet. 42(2):132-136(2010). Gudmundsson, J., et al. Nat. Genet. 41(10):1122-1126(2009). Panagopoulos, I., et al. Oncol. Rep. 20(5):1029-1033(2008).

Other Notes

Small volumes of anti-P5F1B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for P5F1B purchase

MBS6330322 is an antibody targeting P5F1B. Also known as P5F1B, CT (POU5F1B, OTF3C, OTF3P1, POU5F1P1, POU5FLC20, POU5FLC8, Putative POU domain, class 5, transcription factor 1B, Octamer-binding protein 3-like, Octamer-binding transcription factor 3-like) (Azide free) (HRP). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for P5F1B. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
putative POU domain, class 5, transcription factor 1B
NCBI Official Synonym Full Names
POU class 5 homeobox 1B
NCBI Official Symbol
POU5F1B
NCBI Official Synonym Symbols
OTF3C; OTF3P1; OCT4PG1; OCT4-PG1; POU5F1P1; POU5F1P4; POU5FLC8; POU5FLC20
UniProt Protein Name
Putative POU domain, class 5, transcription factor 1B
UniProt Synonym Protein Names
Oct4-pg1; Octamer-binding protein 3-like; Octamer-binding transcription factor 3-like
UniProt Gene Name
POU5F1B
UniProt Synonym Gene Names
OCT4PG1; OTF3C; OTF3P1; POU5F1P1; POU5FLC20; POU5FLC8
UniProt Entry Name
P5F1B_HUMAN
NCBI Protein Information
putative POU domain, class 5, transcription factor 1B; POU 5 domain protein; POU class 5 homeobox 1 pseudogene 1; POU domain transcription factor Oct-4; octamer binding protein 3_like sequence; POU domain transcription factor OCT4-pg1; POU domain, class 5

UniProt Comments for P5F1B

POU5F1B: Shows weak transcriptional activator activity. Belongs to the POU transcription factor family. Class- 5 subfamily.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 8q24.21

Cellular Component: nucleus

Molecular Function: sequence-specific DNA binding; transcription factor activity

Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-P5F1B antibody

Organs/Tissues associated with anti-P5F1B antibody

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