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P67phox Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS616212

P67PHOX is a 6-element fingerprint that provides a signature for neutrophil cytosol factor 2 (p67phox). Regulation of the respiratory burst takes place at the phagocytic vacuole. The process is mediated by NADPH oxidase, which transports electrons across the… More details

$1095 / 0.2 mL +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
p67phox, Polyclonal Antibody
Full Product Name
p67phox
Product Synonym Names
Anti -p67phox
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid, 0.02% azide.
Purity/Purification
Serum
Neat serum
Specificity
Recognizes human p67-phox at 65kD; a degradation product is detected at 45kD. Species Crossreactivity: Reported for bovine.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
GST fusion protein corresponding to full-length human p67-phox.
Applications Tested/Suitable for anti-p67phox antibody
Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)
Application Notes for anti-p67phox antibody
Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry.
Dilution: Western Blot: 1:500-1:2000 detects p67-phox in RIPA lysates from HL-60 cells. Note: p67-phox is highly susceptible to proteases.
Immunoprecipitation: 4ul immunoprecipitated p67-phox from 500ug of HL-60 RIPA lysate.
Immunocytochemistry: 1:500-1:4000 shows positive cytosolic immunostaining for p67-phox in HL-60 cells fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.

Chemiluminescence of anti-p67phox antibody

WB: HL-60 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with MBS616212 (1:2000 dilution). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system).
Rabbit Anti-P67phox Polyclonal Antibody – Cat# MBS616212

Other Notes

Small volumes of anti-p67phox antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for p67phox purchase

MBS616212 is an antibody targeting p67phox. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for p67phox. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Official Full Name
p67-phox

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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