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PAAF1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6330340

PAAF1 inhibits proteasome 26S assembly and proteolytic activity by impairing the association of the 19S regulatory complex with the 20S core. In case of HIV-1 infection, recruited by viral Tat to the HIV-1 promoter, where it promotes the recruitment of 19S… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
PAAF1, Polyclonal Antibody
Full Product Name
PAAF1, NT (PAAF1, WDR71, Proteasomal ATPase-associated factor 1, Protein G-16, WD repeat-containing protein 71) (AP)
Product Gene Name
anti-PAAF1 antibody
Product Synonym Names
PAAF1; WDR71; Proteasomal ATPase-associated factor 1; Protein G-16; WD repeat-containing protein 71
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
29,982 Da
3D Structure
ModBase 3D Structure for Q9BRP4
Immunogen
PAAF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 115-145 amino acids from the N-terminal region of human PAAF1.
Conjugate
AP
Note
Preservative Free

Product Categories/Family for anti-PAAF1 antibody

Antibodies; WD Repeat Proteins
Applications Tested/Suitable for anti-PAAF1 antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes for anti-PAAF1 antibody
IHC: 1:50-100
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-PAAF1 antibody

Western blot analysis of PAAF1 Antibody (N-term) in A549 cell line lysates (35ug/lane).PAAF1 (arrow) was detected using the purified Pab.
Rabbit Anti-PAAF1 Polyclonal Antibody – Cat# MBS6330340

Immunohistochemistry (IHC) of anti-PAAF1 antibody

PAAF1 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PAAF1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-PAAF1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6330340

Product References and Citations for anti-PAAF1 antibody

Lassot, I., et al. Mol. Cell 25(3):369-383(2007). Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007). Ponnappan, S., et al. Int. J. Biochem. Cell Biol. 39(4):799-809(2007).

Other Notes

Small volumes of anti-PAAF1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PAAF1 purchase

MBS6330340 is an antibody targeting PAAF1. Also known as PAAF1, NT (PAAF1, WDR71, Proteasomal ATPase-associated factor 1, Protein G-16, WD repeat-containing protein 71) (AP). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PAAF1. It may not necessarily be applicable to this product.

NCBI GI #
392513656
NCBI GeneID
80227
NCBI Accession #
NP_001254734.1
UniProt Primary Accession #
Q9BRP4
UniProt Secondary Accession #
Q4G165; Q53HS9; Q7Z500; Q8TBU6; Q9HAB6; A6NDR5; B4DPB0; B7ZAS9
UniProt Related Accession #
Q9BRP4
GenBank Nucleotide #
NM_001267805.1
NCBI Official Full Name
proteasomal ATPase-associated factor 1 isoform 1
NCBI Official Synonym Full Names
proteasomal ATPase associated factor 1
NCBI Official Symbol
PAAF1
NCBI Official Synonym Symbols
PAAF; Rpn14; WDR71
UniProt Protein Name
Proteasomal ATPase-associated factor 1
UniProt Synonym Protein Names
Protein G-16; WD repeat-containing protein 71
Protein Family
Probable proteasomal ATPase-associated factor
UniProt Gene Name
PAAF1
UniProt Synonym Gene Names
WDR71
NCBI Protein Information
proteasomal ATPase-associated factor 1

NCBI Summary for PAAF1

This gene encodes a WD repeat-containing protein involved in regulation of association of proteasome components. During HIV infection, the encoded protein is thought to promote provirus transcription through recruitment of the 19S regulatory complex. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jun 2012]
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UniProt Comments for PAAF1

Inhibits proteasome 26S assembly and proteolytic activity by impairing the association of the 19S regulatory complex with the 20S core. In case of HIV-1 infection, recruited by viral Tat to the HIV-1 promoter, where it promotes the recruitment of 19S regulatory complex through dissociation of the proteasome 26S. This presumably promotes provirus transcription efficiency. Protects SUPT6H from proteasomal degradation.
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Research Articles on PAAF1

1. These results show that intracellular levels of Spt6 are fine-tuned by PAAF1 and proteasome, which is required for HIV-1 transcription and extends to cellular genes implicated in cancer.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PAAF1 antibody

 Reference Product  PubMed Publications
 SUPT6H antibody  >1 publications with PAAF1 and SUPT6H
 PSMD10 antibody  >1 publications with PAAF1 and PSMD10

Diseases associated with anti-PAAF1 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >1 publications with PAAF1 and Inflammation

Organs/Tissues associated with anti-PAAF1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >2 publications with PAAF1 and Blood
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