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Pan T Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9148366

MBS9148366 is an antibody targeting Pan T. Also known as Pan Phospho-T Rabbit pAb. Reactive in Human. Host: Rabbit. More details

$245 / 0.02 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Pan T, Polyclonal Antibody
Full Product Name
Pan Phospho-T Rabbit pAb
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Purity/Purification
Affinity purification
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.|Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Molecular Weight
Observed MW: 10-250kDa/>10kDa
Cross Reactivity
Human, Mouse, Other (Wide Range Predicted)
Immunogen
A synthetic peptide corresponding to a sequence containing phosphorylated T.
Modification
Pan Phospho-Threonine

Product Categories/Family for anti-Pan T antibody

ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-Pan T antibody
Western Blot (WB)
Application Notes for anti-Pan T antibody
WB: 1:500-1:1000

Western Blot (WB) of anti-Pan T antibody

Western blot analysis of lysates from A-431 cells using Pan Phospho-T Rabbit pAb (MBS9148366) at 1:900 dilution. A-431 cells were treated by Calyculin A (50 nM) at 37? for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
Rabbit Anti-Pan T Polyclonal Antibody – Cat# MBS9148366

Western Blot (WB) of anti-Pan T antibody

Western blot analysis of lysates from NIH/3T3 cells using Pan Phospho-T Rabbit pAb (MBS9148366) at 1:900 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37? for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
Rabbit Anti-Pan T Polyclonal Antibody, Western Blot (WB) – Cat# MBS9148366

Other Notes

Small volumes of anti-Pan T antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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