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PCNA Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8126182

Proliferating Cell Nuclear Antigen (PCNA) is a protein only expressed in normal proliferate cells and cancer cells. It is central to both DNA replication and repair. One of the well-established functions for PCNA is its role as the processivity factor for DNA… More details

$370 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PCNA, Polyclonal Antibody
Full Product Name
Anti-PCNA Antibody, Rabbit Polyclonal
Product Gene Name
anti-PCNA antibody
Product Synonym Names
PCNA Antibody, Rabbit PAb, Antigen Affinity Purified; proliferating cell nuclear antigen; Anti-ATLD2 Antibody
Clonality
Polyclonal
Isotype
Rabbit IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid; 0.2um filtered solution in PBS
Purity/Purification
Protein A & Antigen Affinity
Specificity
Human
Rat (Species predicted to react based on 100% sequence homology)
Preparation and Storage
This antibody can be stored at 2 degree C-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Immunogen
A synthetic peptide corresponding to the C-terminus of the Human PCNA
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human PCNA, and purified by antigen affinity chromatography.
Applications Tested/Suitable for anti-PCNA antibody
Western Blot (WB)(FAQ Protocol), Immunoprecipitation (IP)(FAQ Protocol)
Application Notes for anti-PCNA antibody
WB: 1:500-1:2000
IP: 1-5uL/mg of lysate

Western Blot (WB) of anti-PCNA antibody

Anti-PCNA rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLane B: A431 Whole Cell LysateLysates/proteins at 30ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique. Performed under reducing conditions.Predicted band size:29 kDaObserved band size:35 kDa
Rabbit Anti-PCNA Polyclonal Antibody – Cat# MBS8126182

Immunoprecipitation (IP) of anti-PCNA antibody

PCNA was immunoprecipitated using: Lane A:0.5 mg HeLa Whole Cell Lysate Lane B:0.5 mg A431 Whole Cell Lysate 4 uL anti-PCNA rabbit polyclonal antibody and 60ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-PCNA rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 29 kDa Observed band size :35 kDa
Rabbit Anti-PCNA Polyclonal Antibody, Immunoprecipitation (IP) – Cat# MBS8126182

Product References and Citations for anti-PCNA antibody

Balaje. AS. e. al. (2001. Chromatin-boun. PCN. comple. formatio. triggere. b. DN. damag. occur. independen. o. th. AT. gen. produc. i. huma. cells. Nuclei. Acid. Res. 2. (6). 1341-51.Ducou. M. e. al. (2001. Mediatio. o. proliferatin. cel. nuclea. antige. (PCNA)-dependen. DN. replicatio. throug. . conserve. p21(Cip1)-lik. PCNA-bindin. moti. presen. i. th. thir. subuni. o. huma. DN. polymeras. delta. . Bio. Chem. 27. (52). 49258-66.Tetsu. I. e. al. (2002. PCN. clam. facilitate. actio. o. DN. cytosin. methyltransferas. . o. hemimethylate. DNA. Gene. Cells. 7(10). 997-1007.

Other Notes

Small volumes of anti-PCNA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PCNA purchase

MBS8126182 is an antibody targeting PCNA. Also known as Anti-PCNA Antibody, Rabbit Polyclonal. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PCNA. It may not necessarily be applicable to this product.

Protein Family
Probable DNA polymerase sliding clamp

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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