Rabbit Anti-PDCD4 Polyclonal Antibody – Cat# MBS3901627

Detection of human PDCD4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PDCD4 antibody MBS3901627 used for IP at 3 ug/mg lysate. PDCD4 was also immunoprecipitated by rabbit anti-PDCD4 antibodies BL4710 and MBS3901628, which recognize downstream epitopes. For blotting immunoprecipitated PDCD4, MBS3901628 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

PDCD4 Antibody – Rabbit Polyclonal

Name: PDCD4 Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS3901627
Price: 250 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3901627

Programmed cell death 4 (PDCD4) has been identified as a tumor suppressor that is able to inhibit tumor invasion and neoplastic transformation. The tumor suppressor activity of PDCD4 is achieved via its ability to inhibit translation initiation of proteins… More details

$250 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

PDCD4, Polyclonal Antibody

Full Product Name

Rabbit anti-PDCD4 Antibody, Affinity Purified

Product Gene Name

anti-PDCD4 antibody

Product Synonym Names

programmed cell death 4 (neoplastic transformation inhibitor); protein 197/15a; Nuclear antigen H731-like; nuclear antigen H731; neoplastic transformation inhibitor protein; H731; programmed cell death protein 4; Programmed cell death protein 4; programmed cell death 4

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human

Form/Format

Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide

Purity/Purification

Antigen Affinity Purified

Concentration

1000ug/ml (lot specific)

Preparation and Storage

Store at 2-8 degree C
Shelf Life: 1 year from date of receipt

Molecular Weight

51,735 Da

Chromosome Location

Chromosome: 10; NC_000010.10 (112631553..112659764). Location: 10q24

OMIM

608610

3D Structure

ModBase 3D Structure for Q53EL6

Predicted Reactivity

Mouse, Rat, Orangutan

Immunogen

Between 125 and 175

Conjugation

Unconjugated

Product Categories/Family for anti-PDCD4 antibody

Primary Antibodies

Applications Tested/Suitable for anti-PDCD4 antibody

Immunoprecipitation (IP)

Application Notes for anti-PDCD4 antibody

IP: 6ug/mg lysate

Other Notes

Small volumes of anti-PDCD4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PDCD4 purchase

MBS3901627 is an antibody targeting PDCD4. Also known as Rabbit anti-PDCD4 Antibody, Affinity Purified. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PDCD4. It may not necessarily be applicable to this product.

NCBI GI #

21735596

NCBI GeneID

27250

NCBI Accession #

NP_055271.2

UniProt Primary Accession #

Q53EL6

UniProt Secondary Accession #

O15501; Q5VZS6; Q6PJI5; Q8TAR5; Q99834; B2RCV4; B5ME91

UniProt Related Accession #

Q53EL6

GenBank Nucleotide #

NM_014456.4

NCBI Official Full Name

programmed cell death protein 4 isoform 1

NCBI Official Synonym Full Names

programmed cell death 4 (neoplastic transformation inhibitor)

NCBI Official Symbol

PDCD4

NCBI Official Synonym Symbols

H731

UniProt Protein Name

Programmed cell death protein 4

UniProt Synonym Protein Names

Neoplastic transformation inhibitor protein; Nuclear antigen H731-like; Protein 197/15a

Protein Family

Programmed cell death protein

UniProt Gene Name

PDCD4

UniProt Synonym Gene Names

H731

UniProt Entry Name

PDCD4_HUMAN

NCBI Protein Information

programmed cell death protein 4; protein 197/15a; nuclear antigen H731; neoplastic transformation inhibitor protein

NCBI Summary for PDCD4

This gene is a tumor suppressor and encodes a protein that binds to the eukaryotic translation initiation factor 4A1 and inhibits its function by preventing RNA binding. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2010]

UniProt Comments for PDCD4

PDCD4: protein localized to the nucleus in proliferating cells that seems to possess a tumor suppressor activity. Directly interacts with the RNA helicase eIF4A and inhibits protein synthesis by interfering with the assembly of the cap-dependent translation initiation complex. Suppresses carbonic anhydrase type II protein expression in carcinoid cell lines. Since tumor cells require a high bicarbonate flux for their growth, carbonic anhydrase suppression results in growth inhibition. Expression of this gene is modulated by cytokines in natural killer and T cells. The gene product is thought to play a role in apoptosis but the specific role has not yet been determined. Two differentially spliced isoforms have been identified.

Protein type: Tumor suppressor; Apoptosis

Chromosomal Location of Human Ortholog: 10q24

Cellular Component: nucleoplasm; cytoplasm; nucleus; cytosol

Molecular Function: protein binding; RNA binding

Biological Process: negative regulation of JNK activity; apoptosis; cell aging; negative regulation of transcription, DNA-dependent; negative regulation of cell cycle

Research Articles on PDCD4

1. Loss of PDCD4 indicates chemoresistance in pancreatic cancer.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PDCD4 antibody

Reference Product	PubMed Publications
RPS13 antibody	>1 publications with PDCD4 and RPS13
RPL5 antibody	>1 publications with PDCD4 and RPL5
EIF4G1 antibody	>1 publications with PDCD4 and EIF4G1

Pathways associated with anti-PDCD4 antibody

Products by Pathway	Pathway Diagram
Proteoglycans In Cancer Pathway antibodies	Proteoglycans In Cancer Pathway Diagram
Proteoglycans In Cancer Pathway antibodies	Proteoglycans In Cancer Pathway Diagram
MTOR Signaling Pathway antibodies	MTOR Signaling Pathway Diagram

Diseases associated with anti-PDCD4 antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>179 publications with PDCD4 and Neoplasms
Carcinoma Antibodies	>80 publications with PDCD4 and Carcinoma
Cell Transformation, Neoplastic Antibodies	>35 publications with PDCD4 and Cell Transformation, Neoplastic
Neoplasm Metastasis Antibodies	>32 publications with PDCD4 and Neoplasm Metastasis
Neoplasm Invasiveness Antibodies	>26 publications with PDCD4 and Neoplasm Invasiveness
Lung Neoplasms Antibodies	>18 publications with PDCD4 and Lung Neoplasms
Liver Neoplasms Antibodies	>16 publications with PDCD4 and Liver Neoplasms
Inflammation Antibodies	>16 publications with PDCD4 and Inflammation
Neoplasms, Experimental Antibodies	>16 publications with PDCD4 and Neoplasms, Experimental
Liver Diseases Antibodies	>15 publications with PDCD4 and Liver Diseases

Organs/Tissues associated with anti-PDCD4 antibody

Organ/Tissue Name	Pubmed Publications
Lung Antibodies	>27 publications with PDCD4 and Lung
Liver Antibodies	>24 publications with PDCD4 and Liver
Blood Antibodies	>23 publications with PDCD4 and Blood
Muscle Antibodies	>18 publications with PDCD4 and Muscle
Lymph Antibodies	>16 publications with PDCD4 and Lymph
Lymph Node Antibodies	>16 publications with PDCD4 and Lymph Node
Skin Antibodies	>16 publications with PDCD4 and Skin
Brain Antibodies	>15 publications with PDCD4 and Brain
Prostate Antibodies	>12 publications with PDCD4 and Prostate
Vascular Antibodies	>11 publications with PDCD4 and Vascular