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PDIA3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS626146

PDIA3 is the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
PDIA3, Polyclonal Antibody
Full Product Name
PDIA3, CT (Protein Disulfide-isomerase A3, Disulfide Isomerase ER-60, Endoplasmic Reticulum Resident Protein 60, ER Protein 60, ERp60, 58kD Microsomal Protein, p58, Endoplasmic Reticulum Resident Protein 57, ER Protein 57, ERp57, 58kD Glucose-regulated Pr
Product Gene Name
anti-PDIA3 antibody
Product Synonym Names
Anti -PDIA3, CT (Protein Disulfide-isomerase A3, Disulfide Isomerase ER-60, Endoplasmic Reticulum Resident Protein 60, ER Protein 60, ERp60, 58kD Microsomal Protein, p58, Endoplasmic Reticulum Resident Protein 57, ER Protein 57, ERp57, 58kD Glucose-regulated Pr
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, 0.109% sodium azide.
Purity/Purification
Purified
Purified by ammonium sulfate precipitation.
Specificity
Recognizes human PDIA3.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
56,782 Da
Chromosome Location
Chromosome: 15; NC_000015.9 (44038590..44064804). Location: 15q15
OMIM
602046
3D Structure
ModBase 3D Structure for P30101
Immunogen
Synthetic peptide selected from the C-terminal region of human PDIA3 (KLH).

Product Categories/Family for anti-PDIA3 antibody

Antibodies; Abs to Nuclear Proteins
Applications Tested/Suitable for anti-PDIA3 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-PDIA3 antibody
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100

Western Blot (WB) of anti-PDIA3 antibody

Western blot analysis of PDIA3 Antibody (C-term) in A2058 cell line lysates (35ug/lane). PDIA3 (arrow) was detected using the purified Pab.
Rabbit Anti-PDIA3 Polyclonal Antibody – Cat# MBS626146

Western Blot (WB) of anti-PDIA3 antibody

Western blot analysis of PDIA3 (arrow) using rabbit polyclonal PDIA3 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PDIA3 gene (Lane 2) .
Rabbit Anti-PDIA3 Polyclonal Antibody, Western Blot (WB) – Cat# MBS626146

Immunohistochemistry (IHC) of anti-PDIA3 antibody

Formalin-fixed and paraffin-embedded human lung carcinoma reacted with PDIA3 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-PDIA3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS626146

Flow Cytometry (FC/FACS) of anti-PDIA3 antibody

PDIA3 Antibody (C-term) flow cytometric analysis of NCI-H292 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-PDIA3 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS626146

Other Notes

Small volumes of anti-PDIA3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PDIA3 purchase

MBS626146 is an antibody targeting PDIA3. Also known as PDIA3, CT (Protein Disulfide-isomerase A3, Disulfide Isomerase ER-60, Endoplasmic Reticulum Resident Protein 60, ER Protein 60, ERp60, 58kD Microsomal Protein, p58, Endoplasmic Reticulum Resident Protein 57, ER Protein 57, ERp57, 58kD Glucose-regulated Pr. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PDIA3. It may not necessarily be applicable to this product.

NCBI GI #
21361657
NCBI GeneID
2923
NCBI Accession #
NP_005304.3
UniProt Primary Accession #
P30101
UniProt Secondary Accession #
Q13453; Q14255; Q8IYF8; Q9UMU7
UniProt Related Accession #
P30101; AAB37397
GenBank Nucleotide #
NM_005313.4
NCBI Official Full Name
protein disulfide-isomerase A3
NCBI Official Synonym Full Names
protein disulfide isomerase family A, member 3
NCBI Official Symbol
PDIA3
NCBI Official Synonym Symbols
P58; ER60; ERp57; ERp60; ERp61; GRP57; GRP58; PI-PLC; HsT17083
UniProt Protein Name
Protein disulfide-isomerase A3
UniProt Synonym Protein Names
58 kDa glucose-regulated protein; 58 kDa microsomal protein; p58; Disulfide isomerase ER-60; Endoplasmic reticulum resident protein 57; ER protein 57; ERp57; Endoplasmic reticulum resident protein 60
Protein Family
Protein disulfide-isomerase
UniProt Gene Name
PDIA3
UniProt Synonym Gene Names
ERP57; ERP60; GRP58
UniProt Entry Name
PDIA3_HUMAN
NCBI Protein Information
protein disulfide-isomerase A3; ER protein 57; ER protein 60; OTTHUMP00000041709; phospholipase C-alpha; 58 kDa microsomal protein; disulfide isomerase ER-60; endoplasmic reticulum P58; 58 kDa glucose-regulated protein; glucose regulated protein, 58kDa; protein disulfide isomerase-associated 3; endoplasmic reticulum resident protein 57; endoplasmic reticulum resident protein 60

NCBI Summary for PDIA3

This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates. [provided by RefSeq]
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UniProt Comments for PDIA3

GRP58: a protein disulfide isomerase found in the endoplasmic reticulum lumen. Catalyzes the rearrangement of both intrachain and interchain disulfide bonds in proteins to form the native structures. An essential component of the peptide-loading complex of the major histocompatibility complex class I pathway.

Protein type: Chaperone; Phospholipase; Apoptosis; Protease; EC 5.3.4.1; Endoplasmic reticulum; Isomerase

Chromosomal Location of Human Ortholog: 15q15

Cellular Component: cell surface; focal adhesion; smooth endoplasmic reticulum; endoplasmic reticulum; endoplasmic reticulum lumen; melanosome; nucleus

Molecular Function: disulfide oxidoreductase activity; protein binding; cysteine-type endopeptidase activity; protein disulfide isomerase activity; phospholipase C activity

Biological Process: antigen processing and presentation of peptide antigen via MHC class I; cellular protein metabolic process; protein folding; protein import into nucleus; cell redox homeostasis; antigen processing and presentation of exogenous peptide antigen via MHC class I; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; protein amino acid N-linked glycosylation via asparagine; signal transduction; proteolysis; post-translational protein modification; protein retention in ER
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Research Articles on PDIA3

1. HLA-I, TAP1, CNX, LMP7, Erp57, Tapasin and ERAP1 were down-regulated in 68%, 44%, 48%, 40%, 52%, 32% and 20% of esophageal squamous cell carcinoma lesions then, respectively.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PDIA3 antibody

 Reference Product  PubMed Publications
 TAP1 antibody  >13 publications with PDIA3 and TAP1
 STAT3 antibody  >7 publications with PDIA3 and STAT3
 HLA-A antibody  >1 publications with PDIA3 and HLA-A
 ERP27 antibody  >1 publications with PDIA3 and ERP27
 ERO1L antibody  >1 publications with PDIA3 and ERO1L
 CANX antibody  >1 publications with PDIA3 and CANX
 CALR antibody  >1 publications with PDIA3 and CALR

Pathways associated with anti-PDIA3 antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immunity Signaling Pathway antibodies  Adaptive Immunity Signaling Pathway Diagram
 Antigen Presentation: Folding, Assembly And Peptide Loading Of Class I MHC Pathway antibodies  Antigen Presentation: Folding, Assembly And Peptide Loading Of Class I MHC Pathway Diagram
 Antigen Processing And Presentation Pathway antibodies  Antigen Processing And Presentation Pathway Diagram
 Antigen Processing And Presentation Pathway antibodies  Antigen Processing And Presentation Pathway Diagram
 Asparagine N-linked Glycosylation Pathway antibodies  Asparagine N-linked Glycosylation Pathway Diagram
 Calnexin/calreticulin Cycle Pathway antibodies  Calnexin/calreticulin Cycle Pathway Diagram
 Class I MHC Mediated Antigen Processing & Presentation Pathway antibodies  Class I MHC Mediated Antigen Processing & Presentation Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 N-glycan Trimming In The ER And Calnexin/Calreticulin Cycle Pathway antibodies  N-glycan Trimming In The ER And Calnexin/Calreticulin Cycle Pathway Diagram

Diseases associated with anti-PDIA3 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >35 publications with PDIA3 and Neoplasms
 Carcinoma Antibodies  >13 publications with PDIA3 and Carcinoma
 Liver Diseases Antibodies  >6 publications with PDIA3 and Liver Diseases
 Neuroectodermal Tumors Antibodies  >6 publications with PDIA3 and Neuroectodermal Tumors
 Nervous System Diseases Antibodies  >5 publications with PDIA3 and Nervous System Diseases
 Liver Neoplasms Antibodies  >5 publications with PDIA3 and Liver Neoplasms
 Carcinoma, Hepatocellular Antibodies  >5 publications with PDIA3 and Carcinoma, Hepatocellular
 Drug Toxicity Antibodies  >5 publications with PDIA3 and Drug Toxicity
 Adenocarcinoma Antibodies  >5 publications with PDIA3 and Adenocarcinoma
 Inflammation Antibodies  >5 publications with PDIA3 and Inflammation

Organs/Tissues associated with anti-PDIA3 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >21 publications with PDIA3 and Blood
 Liver Antibodies  >15 publications with PDIA3 and Liver
 Kidney Antibodies  >10 publications with PDIA3 and Kidney
 Lung Antibodies  >8 publications with PDIA3 and Lung
 Intestine Antibodies  >8 publications with PDIA3 and Intestine
 Brain Antibodies  >8 publications with PDIA3 and Brain
 Muscle Antibodies  >5 publications with PDIA3 and Muscle
 Bone Antibodies  >5 publications with PDIA3 and Bone
 Vascular Antibodies  >5 publications with PDIA3 and Vascular
 Testis Antibodies  >4 publications with PDIA3 and Testis
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