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PDIA3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6492464

MBS6492464 is an antibody targeting PDIA3. Also known as PDIA3, ID (Protein Disulfide-isomerase A3, Disulfide Isomerase ER-60, Endoplasmic Reticulum Resident Protein 60, ER Protein 60, ERp60, 58kD Microsomal Protein, p58, Endoplasmic Reticulum Resident… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PDIA3, Polyclonal Antibody
Full Product Name
PDIA3, ID (Protein Disulfide-isomerase A3, Disulfide Isomerase ER-60, Endoplasmic Reticulum Resident Protein 60, ER Protein 60, ERp60, 58kD Microsomal Protein, p58, Endoplasmic Reticulum Resident Protein 57, ER Protein 57, ERp57, 58kD Glucose-regulated Pr
Product Gene Name
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.
Purity/Purification
Purified by Ammonium Sulfate Precipitation.
Specificity
Recognizes human PDIA3.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
56,782 Da
OMIM
602046
3D Structure
ModBase 3D Structure for P30101
Immunogen
Synthetic peptide selected from the center region of human PDIA3 (KLH).
Conjugate
MaxLight490
Note
Preservative Free

Product Categories/Family for anti-PDIA3 antibody

Applications Tested/Suitable for anti-PDIA3 antibody
Western Blot (WB), FLISA
Application Notes for anti-PDIA3 antibody
WB: 1:50-1:100
FLISA: 1:1,000
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-PDIA3 antibody

Western blot analysis of PDIA3 Antibody (Center) in A2058, A375, Ramos cell line lysates (35ug/lane). PDIA3 (arrow) was detected using the purified Pab.(2ug/ml)
Rabbit Anti-PDIA3 Polyclonal Antibody – Cat# MBS6492464

Western Blot (WB) of anti-PDIA3 antibody

Western blot analysis of PDIA3 (arrow) using rabbit polyclonal PDIA3 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PDIA3 gene (Lane 2).
Rabbit Anti-PDIA3 Polyclonal Antibody, Western Blot (WB) – Cat# MBS6492464

Immunohistochemistry (IHC) of anti-PDIA3 antibody

Formalin-fixed and paraffin-embedded human lung carcinoma reacted with PDIA3 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-PDIA3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6492464

Flow Cytometry (FC/FACS) of anti-PDIA3 antibody

Flow cytometric analysis of widr cells using PDIA3 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-PDIA3 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6492464

Product References and Citations for anti-PDIA3 antibody

1. Vigneron, N., et.al., Eur. J. Immunol. 39(9), 2371-2376 (2009). 2. Xu, D., et.al., Am. J. Physiol. Lung Cell Mol. Physiol. 297(1), L44-L51 (2009).

Other Notes

Small volumes of anti-PDIA3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PDIA3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
protein disulfide-isomerase A3
NCBI Official Synonym Full Names
protein disulfide isomerase family A member 3
NCBI Official Symbol
PDIA3
NCBI Official Synonym Symbols
P58; ER60; ERp57; ERp60; ERp61; GRP57; GRP58; PI-PLC; HsT17083; HEL-S-269; HEL-S-93n
UniProt Protein Name
Protein disulfide-isomerase A3
UniProt Synonym Protein Names
58 kDa glucose-regulated protein; 58 kDa microsomal protein; p58; Disulfide isomerase ER-60; Endoplasmic reticulum resident protein 57; ER protein 57; ERp57; Endoplasmic reticulum resident protein 60
UniProt Gene Name
PDIA3
UniProt Synonym Gene Names
ERP57; ERP60; GRP58; p58; ER protein 57; ERp57; ER protein 60; ERp60
UniProt Entry Name
PDIA3_HUMAN
NCBI Protein Information
protein disulfide-isomerase A3

NCBI Summary for PDIA3

This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates. This protein also functions as a molecular chaperone that prevents the formation of protein aggregates. [provided by RefSeq, Dec 2016]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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