Rabbit Anti-PEMT Polyclonal Antibody – Cat# MBS6492655

Western blot analysis of PEMT Antibody (C-term) in T47D cell line lysates (35ug/lane). PEMT (arrow) was detected using the purified Pab.

PEMT Antibody – Rabbit Polyclonal

Name: PEMT Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS6492655
Price: 1060 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6492655

MBS6492655 is an antibody targeting PEMT. Also known as PEMT, CT (PEAMT, PEMT2, PEMPT, PNMT, Phosphatidylethanolamine N-methyltransferase) (PE). Reactive in Human. Host: Rabbit. More details

$1060 / 0.2 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

PEMT, Polyclonal Antibody

Full Product Name

PEMT, CT (PEAMT, PEMT2, PEMPT, PNMT, Phosphatidylethanolamine N-methyltransferase) (PE)

Product Gene Name

anti-PEMT antibody

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human

Form/Format

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

Purity/Purification

Purified by Protein A Affinity Chromatography.

Specificity

Recognizes human PEMT.

Preparation and Storage

Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Molecular Weight

OMIM

602391

3D Structure

ModBase 3D Structure for Q9UBM1

Immunogen

Synthetic peptide selected from the C-terminal region of human PEMT (KLH).

Conjugate

Note

Preservative Free

Special Handling

Light sensitive

Product Categories/Family for anti-PEMT antibody

Antibodies; Enzymes; Methyltransferase

Applications Tested/Suitable for anti-PEMT antibody

Western Blot (WB), FLISA

Application Notes for anti-PEMT antibody

WB: 1:50-1:100
FLISA: 1:1000
Applications are based on unconjugated antibody.

Product References and Citations for anti-PEMT antibody

1. Walkey, C.J., Biochim. Biophys. Acta 1436: 405-412 (1999). 2. Shields, D.J., Biochim. Biophys. Acta 1532: 105-114 (2001). 3. Hu, R.-M., Proc. Natl. Acad. Sci. USA 97: 9543-9548 (2000).

Other Notes

Small volumes of anti-PEMT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PEMT. It may not necessarily be applicable to this product.

NCBI GI #

22538482

NCBI GeneID

10400

NCBI Accession #

NP_009100

UniProt Primary Accession #

Q9UBM1

UniProt Related Accession #

Q9UBM1

GenBank Nucleotide #

BC050593

NCBI Official Full Name

phosphatidylethanolamine N-methyltransferase isoform 2

NCBI Official Synonym Full Names

phosphatidylethanolamine N-methyltransferase

NCBI Official Symbol

PEMT

NCBI Official Synonym Symbols

PLMT; PNMT; PEAMT; PEMPT; PEMT2

UniProt Protein Name

Phosphatidylethanolamine N-methyltransferase

UniProt Synonym Protein Names

PEMT2

Protein Family

PEMT/PEM2 methyltransferase family protein

UniProt Gene Name

PEMT

UniProt Synonym Gene Names

PEMPT; PNMT; PEAMT; PEMT

UniProt Entry Name

PEMT_HUMAN

NCBI Protein Information

phosphatidylethanolamine N-methyltransferase

NCBI Summary for PEMT

Phosphatidylcholine (PC) is the most abundant mammalian phospholipid. This gene encodes an enzyme which converts phosphatidylethanolamine to phosphatidylcholine by sequential methylation in the liver. Another distinct synthetic pathway in nucleated cells converts intracellular choline to phosphatidylcholine by a three-step process. The protein isoforms encoded by this gene localize to the endoplasmic reticulum and mitochondria-associated membranes. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, May 2012]

Research Articles on PEMT

1. Study found three gene variants (CLOCK-rs4864548, PEMT-rs936108, and GHRELIN-rs696217) that exhibited uncorrected gene-by-sleep duration interactions in relation to BMI z-scores in a cohort of New Zealand European children. However, no interactions were identified in percentage body fat differences. Notably, these interactions are evident without detectable effects on sleep duration.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.