• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

PFKFB2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS7048863

MBS7048863 is an antibody targeting PFKFB2. Also known as PFKFB2 Antibody. Reactive in Human. Host: Rabbit. Synthesis and degradation of fructose 2, 6-bisphosphate. More details

$230 / 0.05 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PFKFB2, Polyclonal Antibody
Full Product Name
PFKFB2 Antibody
Product Gene Name
Product Synonym Names
6-phosphofructo-2-kinase/fructose-2; 6-bisphosphatase 26PF-2-K/Fru-2; 6-P2ase 2PFK/FBPase 2; 6PF-2-K/Fru-2; 6-P2ase heart-type isozyme; Including the following 2 domains: ; 6-phosphofructo-2-kinase (EC: 2.7.1.105; PFKFB2
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid
Purity/Purification
>95%, Protein G purified
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
Matching Pairs
Unconjugated Antibody: PFKFB2 (MBS7048863)
HRP Conjugated Antibody: PFKFB2 (MBS7048864)
Matching Pairs
Unconjugated Antibody: PFKFB2 (MBS7048863)
FITC Conjugated Antibody: PFKFB2 (MBS7048865)
Molecular Weight
54,406 Da
OMIM
AJ005577 Genomic DNA
3D Structure
ModBase 3D Structure for O60825
Immunogen
Recombinant Human 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 2 protein (360-505AA)
Preservative
0.03% Proclin 300
Constituents
50% Glycerol, 0.01M PBS, PH 7.4
Conjugation
Non-conjugated
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-50953 / sc-50956
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-PFKFB2 antibody
ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-PFKFB2 antibody
IHC: 1: 20-1: 200
IF: 1: 50-1: 200

Immunohistochemistry (IHC) of anti-PFKFB2 antibody

Immunohistochemistry of paraffin-embedded human lung cancer using MBS7048863 at dilution of 1:100
Rabbit Anti-PFKFB2 Polyclonal Antibody – Cat# MBS7048863

Immunofluorescence (IF) of anti-PFKFB2 antibody

Immunofluorescent analysis of HepG2 cells using MBS7048863 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Rabbit Anti-PFKFB2 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS7048863

Western Blot (WB) of anti-PFKFB2 antibody

Western Blot
Positive WB detected in: K562 whole cell lysate,Rat kidney tissue,Rat brain tissue
All lanes: PFKFB2 antibody at 3ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 59,55 KDa
Observed band size: 59,55 KDa
Rabbit Anti-PFKFB2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS7048863

Other Notes

Small volumes of anti-PFKFB2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PFKFB2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2 isoform b
NCBI Official Synonym Full Names
6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2
NCBI Official Symbol
PFKFB2
NCBI Official Synonym Symbols
PFK-2/FBPase-2
UniProt Protein Name
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2
UniProt Synonym Protein Names
6PF-2-K/Fru-2,6-P2ase heart-type isozymeIncluding the following 2 domains:6-phosphofructo-2-kinase (EC:2.7.1.105)Fructose-2,6-bisphosphatase (EC:3.1.3.46)
UniProt Gene Name
PFKFB2
UniProt Synonym Gene Names
6PF-2-K/Fru-2,6-P2ase 2; PFK/FBPase 2
UniProt Entry Name
F262_HUMAN
NCBI Protein Information
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2

NCBI Summary for PFKFB2

The protein encoded by this gene is involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate, and a fructose-2,6-biphosphatase activity that catalyzes the degradation of fructose-2,6-bisphosphate. This protein regulates fructose-2,6-bisphosphate levels in the heart, while a related enzyme encoded by a different gene regulates fructose-2,6-bisphosphate levels in the liver and muscle. This enzyme functions as a homodimer. Two transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Show more

UniProt Comments for PFKFB2

PFKFB2: a bifunctional heterodimeric enzyme involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. Regulates fructose-2,6-bisphosphate levels in the heart, while a related enzyme encoded by a different gene regulates fructose-2,6-bisphosphate levels in the liver and muscle. Two splice-variant isoforms have been described.

Protein type: Carbohydrate Metabolism - fructose and mannose; EC 2.7.1.105; EC 3.1.3.46; Kinase, other; Motility/polarity/chemotaxis; Phosphatase (non-protein)

Chromosomal Location of Human Ortholog: 1q31

Cellular Component: cytosol

Molecular Function: 6-phosphofructo-2-kinase activity; protein binding; protein kinase binding

Biological Process: glycolysis
Show more

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000