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PFKP Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6008356

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in… More details

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Product Overview

Online Datasheet in PDF
Product Name
PFKP, Polyclonal Antibody
Full Product Name
PFKP, CT (PFKP, PFKF, 6-phosphofructokinase type C, 6-phosphofructokinase, platelet type, Phosphofructo-1-kinase isozyme C, Phosphofructokinase 1, Phosphohexokinase)
Product Gene Name
anti-PFKP antibody
Product Synonym Names
Anti -PFKP, CT (PFKP, PFKF, 6-phosphofructokinase type C, 6-phosphofructokinase, platelet type, Phosphofructo-1-kinase isozyme C, Phosphofructokinase 1, Phosphohexokinase)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Specificity
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
85,596 Da
Chromosome Location
Chromosome: 10; NC_000010.10 (3109667..3178997). Location: 10p15.3-p15.2
OMIM
171840
3D Structure
ModBase 3D Structure for Q01813
Immunogen
PFKP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 755~784 amino acids from the C-terminal region of human PFKP.

Product Categories/Family for anti-PFKP antibody

Antibodies; Abs to Protein Kinases
Applications Tested/Suitable for anti-PFKP antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-PFKP antibody
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100

Western Blot (WB) of anti-PFKP antibody

Western Blot analysis in T47D cell line lysates (35ug/lane) using MBS6008356. This demonstrates that MBS6008356 detected the PFKP protein (arrow).
Rabbit Anti-PFKP Polyclonal Antibody – Cat# MBS6008356

Immunohistochemistry (IHC) of anti-PFKP antibody

Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human cancer tissue reacted with MBS6008356, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of MBS6008356 for immunohistochemistry. BC = breast carcinoma; HC = hepatocarcinoma.
Rabbit Anti-PFKP Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6008356

Other Notes

Small volumes of anti-PFKP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PFKP purchase

MBS6008356 is an antibody targeting PFKP. Also known as PFKP, CT (PFKP, PFKF, 6-phosphofructokinase type C, 6-phosphofructokinase, platelet type, Phosphofructo-1-kinase isozyme C, Phosphofructokinase 1, Phosphohexokinase). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PFKP. It may not necessarily be applicable to this product.

NCBI GI #
71297291
NCBI GeneID
5214
NCBI Accession #
AAH43156.1
UniProt Primary Accession #
Q01813
UniProt Secondary Accession #
Q5VSR7; Q5VSR8; B3KS15
UniProt Related Accession #
Q01813
NCBI Official Full Name
PFKP protein
NCBI Official Synonym Full Names
phosphofructokinase, platelet
NCBI Official Symbol
PFKP
NCBI Official Synonym Symbols
PFKF; PFK-C
UniProt Protein Name
6-phosphofructokinase type C
UniProt Synonym Protein Names
6-phosphofructokinase, platelet type; Phosphofructo-1-kinase isozyme C; PFK-C; Phosphofructokinase 1; Phosphohexokinase
Protein Family
ATP-dependent 6-phosphofructokinase
UniProt Gene Name
PFKP
UniProt Synonym Gene Names
PFKF; PFK-C
UniProt Entry Name
K6PP_HUMAN
NCBI Protein Information
6-phosphofructokinase type C; phosphohexokinase; phosphofructokinase 1; phosphofructo-1-kinase isozyme C; Phosphofructokinase, platelet type; 6-phosphofructokinase, platelet type

NCBI Summary for PFKP

The PFKP gene encodes the platelet isoform of phosphofructokinase (PFK) (ATP:D-fructose-6-phosphate-1-phosphotransferase, EC 2.7.1.11). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. The PFKP gene, which maps to chromosome 10p, is also expressed in fibroblasts. See also the muscle (PFKM; MIM 610681) and liver (PFKL; MIM 171860) isoforms of phosphofructokinase, which map to chromosomes 12q13 and 21q22, respectively. Vora (1981) [PubMed 6451249] determined that full tetrameric phophofructokinase enzyme expressed in platelets can be composed of subunits P4, P3L, and P2L2.[supplied by OMIM, Mar 2008]
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UniProt Comments for PFKP

PFKP: phosphofructokinase, platelet type. An ubiquitous metabolic enzyme involved in the synthesis and degradation of fructose 2,6-bisphosphate. Key control step of glycolysis. An allosteric enzyme activated by ADP, AMP, or fructose bisphosphate and inhibited by ATP or citrate. Activity: ATP D-fructose 6-phosphate = ADP D-fructose 1,6-bisphosphate. The holoenzyme consists of 4 subunits. The liver and muscle enzymes are homo-tetramers of four liver or muscle isoforms, respectively. The red blood cell enzyme consists hetero-tetramers of the muscle and liver isoforms. A subunit composition with a higher proportion of platelet type subunits is found in platelets, brain and fibroblasts.

Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - galactose; Carbohydrate Metabolism - fructose and mannose; Kinase, other; Carbohydrate Metabolism - pentose phosphate pathway; EC 2.7.1.11

Chromosomal Location of Human Ortholog: 10p15.3-p15.2

Cellular Component: membrane; cytosol; nucleus

Molecular Function: metal ion binding; protein complex binding; ATP binding; 6-phosphofructokinase activity

Biological Process: glycolysis; carbohydrate metabolic process; carbohydrate phosphorylation; glucose metabolic process; pathogenesis; fructose 6-phosphate metabolic process
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Research Articles on PFKP

1. expression of TARDBP is significantly elevated in HCC and it regulates the expression of PFKP, the rate-limiting enzyme for glycolysis, through negative regulation of microRNA 520s (miR-520s).

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PFKP antibody

 Reference Product  PubMed Publications
 ALDOA antibody  >2 publications with PFKP and ALDOA
 PFKL antibody  >1 publications with PFKP and PFKL
 PFKFB3 antibody  >1 publications with PFKP and PFKFB3
 HK2 antibody  >1 publications with PFKP and HK2
 PFKM antibody  >1 publications with PFKP and PFKM

Pathways associated with anti-PFKP antibody

 Products by Pathway  Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Conversion Of Glucose To Acetyl CoA And Entry Into The TCA Cycle Pathway antibodies  Conversion Of Glucose To Acetyl CoA And Entry Into The TCA Cycle Pathway Diagram
 Fructose And Mannose Metabolism Pathway antibodies  Fructose And Mannose Metabolism Pathway Diagram
 Fructose And Mannose Metabolism Pathway antibodies  Fructose And Mannose Metabolism Pathway Diagram
 Galactose Metabolism Pathway antibodies  Galactose Metabolism Pathway Diagram
 Galactose Metabolism Pathway antibodies  Galactose Metabolism Pathway Diagram
 Glucose Metabolism Pathway antibodies  Glucose Metabolism Pathway Diagram

Diseases associated with anti-PFKP antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >4 publications with PFKP and Neoplasms
 Disease Models, Animal Antibodies  >2 publications with PFKP and Disease Models, Animal
 Hypertrophy Antibodies  >1 publications with PFKP and Hypertrophy
 Neoplasms, Experimental Antibodies  >1 publications with PFKP and Neoplasms, Experimental
 Hypertension Antibodies  >1 publications with PFKP and Hypertension
 Breast Neoplasms Antibodies  >1 publications with PFKP and Breast Neoplasms
 Liver Neoplasms Antibodies  >1 publications with PFKP and Liver Neoplasms
 Carcinoma, Hepatocellular Antibodies  >1 publications with PFKP and Carcinoma, Hepatocellular
 Liver Diseases Antibodies  >1 publications with PFKP and Liver Diseases

Organs/Tissues associated with anti-PFKP antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >6 publications with PFKP and Blood
 Muscle Antibodies  >4 publications with PFKP and Muscle
 Ovary Antibodies  >4 publications with PFKP and Ovary
 Liver Antibodies  >4 publications with PFKP and Liver
 Bone Antibodies  >2 publications with PFKP and Bone
 Thyroid Antibodies  >2 publications with PFKP and Thyroid
 Brain Antibodies  >2 publications with PFKP and Brain
 Heart Antibodies  >1 publications with PFKP and Heart
 Skin Antibodies  >1 publications with PFKP and Skin
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