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PGAM4 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6332961

This intronless gene appears to have arisen from a retrotransposition event, yet it is thought to be an expressed, protein-coding gene. The encoded protein is a member of the phosphoglycerate mutase family, a set of enzymes that catalyze the transfer of a… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
PGAM4, Polyclonal Antibody
Full Product Name
PGAM4, NT (PGAM4, PGAM3, Probable phosphoglycerate mutase 4) (FITC)
Product Gene Name
Product Synonym Names
PGAM4; PGAM3; Probable phosphoglycerate mutase 4
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
28,777 Da
OMIM
300567
3D Structure
ModBase 3D Structure for Q8N0Y7
Immunogen
PGAM4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-53 amino acids from the N-terminal region of human PGAM4.
Conjugate
FITC
Note
Preservative Free
Special Handling
Light sensitive

Product Categories/Family for anti-PGAM4 antibody

Applications Tested/Suitable for anti-PGAM4 antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes for anti-PGAM4 antibody
IHC: 1:10-50
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-PGAM4 antibody

PGAM4 Antibody (N-term) western blot analysis in 293,HepG2,Jurkat cell line lysates (35ug/lane).This demonstrates the PGAM4 antibody detected the PGAM4 protein (arrow).
Rabbit Anti-PGAM4 Polyclonal Antibody – Cat# MBS6332961

Immunohistochemistry (IHC) of anti-PGAM4 antibody

PGAM4 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PGAM4 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-PGAM4 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6332961

Product References and Citations for anti-PGAM4 antibody

Olsen, J.V., et al. Cell 127(3):635-648(2006). Kim, S.C., et al. Mol. Cell 23(4):607-618(2006). Marques, A.C., et al. PLoS Biol. 3 (11), E357 (2005): Betran, E., et al. Mol. Biol. Evol. 19(5):654-663(2002). Dierick, H.A., et al. Gene 198 (1-2), 37-41 (1997).

Other Notes

Small volumes of anti-PGAM4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PGAM4 purchase

MBS6332961 is an antibody targeting PGAM4. Also known as PGAM4, NT (PGAM4, PGAM3, Probable phosphoglycerate mutase 4) (FITC). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PGAM4. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
phosphoglycerate mutase 4
NCBI Official Synonym Full Names
phosphoglycerate mutase family member 4
NCBI Official Symbol
PGAM4
NCBI Official Synonym Symbols
PGAM1; PGAM3; PGAM-B; dJ1000K24.1
UniProt Protein Name
Probable phosphoglycerate mutase 4
UniProt Gene Name
PGAM4
UniProt Synonym Gene Names
PGAM3
UniProt Entry Name
PGAM4_HUMAN
NCBI Protein Information
phosphoglycerate mutase 4

UniProt Comments for PGAM4

PGAM4: This intronless gene appears to have arisen from a retrotransposition event, yet it is thought to be an expressed, protein-coding gene. The encoded protein is a member of the phosphoglycerate mutase family, a set of enzymes that catalyze the transfer of a phosphate group from 3-phosphoglycerate to 2-phosphoglycerate. [provided by RefSeq, May 2010]

Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; Phosphatase (non-protein); EC 3.1.3.13; EC 5.4.2.11; EC 5.4.2.4; Isomerase

Chromosomal Location of Human Ortholog: Xq13

Molecular Function: phosphoglycerate mutase activity; bisphosphoglycerate mutase activity; bisphosphoglycerate phosphatase activity

Biological Process: glycolysis; dephosphorylation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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