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PHGDH Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6493116

MBS6493116 is an antibody targeting PHGDH. Also known as PHGDH, NT (D-3-phosphoglycerate Dehydrogenase, 3-PGDH, PGDH3) (MaxLight 405). Reactive in Human. Host: Rabbit. More details

$1025 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PHGDH, Polyclonal Antibody
Full Product Name
PHGDH, NT (D-3-phosphoglycerate Dehydrogenase, 3-PGDH, PGDH3) (MaxLight 405)
Product Gene Name
anti-PHGDH antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.
Purity/Purification
Purified by Ammonium Sulfate Precipitation.
Specificity
Recognizes human PHGDH.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
56,651 Da
OMIM
phenotype 606879
3D Structure
ModBase 3D Structure for O43175
Immunogen
Synthetic peptide selected from the N-terminal region of human PHGDH (KLH).
Conjugate
MaxLight405
Note
Preservative Free

Product Categories/Family for anti-PHGDH antibody

Antibodies; Enzymes; Dehydrogenase
Applications Tested/Suitable for anti-PHGDH antibody
Western Blot (WB), FLISA
Application Notes for anti-PHGDH antibody
WB: 1:50-1:100
FLISA: 1:1,000
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-PHGDH antibody

Western blot analysis of PHGDH Antibody (N-term) in mouse brain tissue lysates (35ug/lane). PHGDH (arrow) was detected using the purified Pab.
Rabbit Anti-PHGDH Polyclonal Antibody – Cat# MBS6493116

Western Blot (WB) of anti-PHGDH antibody

Western blot analysis of PHGDH (arrow) using rabbit polyclonal PHGDH Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PHGDH gene (Lane 2).
Rabbit Anti-PHGDH Polyclonal Antibody, Western Blot (WB) – Cat# MBS6493116

Immunohistochemistry (IHC) of anti-PHGDH antibody

Formalin-fixed and paraffin-embedded human brain tissue reacted with PHGDH Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-PHGDH Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6493116

Flow Cytometry (FC/FACS) of anti-PHGDH antibody

Flow cytometric analysis of hela cells using PHGDH Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-PHGDH Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6493116

Product References and Citations for anti-PHGDH antibody

1. Klomp, L.W., et.al., Am. J. Hum. Genet. 67(6), 1389-1399 (2000).

Other Notes

Small volumes of anti-PHGDH antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PHGDH. It may not necessarily be applicable to this product.

NCBI GI #
23308577
NCBI GeneID
26227
NCBI Accession #
NP_006614
UniProt Primary Accession #
O43175
UniProt Secondary Accession #
Q5SZU3; Q9BQ01; B2RD08
UniProt Related Accession #
O43175
GenBank Nucleotide #
NM_006623
NCBI Official Full Name
D-3-phosphoglycerate dehydrogenase
NCBI Official Synonym Full Names
phosphoglycerate dehydrogenase
NCBI Official Symbol
PHGDH
NCBI Official Synonym Symbols
NLS; PDG; PGD; NLS1; PGAD; PGDH; SERA; 3PGDH; 3-PGDH; PHGDHD; HEL-S-113
UniProt Protein Name
D-3-phosphoglycerate dehydrogenase
UniProt Gene Name
PHGDH
UniProt Synonym Gene Names
PGDH3; 3-PGDH
UniProt Entry Name
SERA_HUMAN
NCBI Protein Information
D-3-phosphoglycerate dehydrogenase; epididymis secretory protein Li 113

UniProt Comments for PHGDH

PHGDH: Defects in PHGDH are the cause of phosphoglycerate dehydrogenase deficiency (PHGDH deficiency). It is characterized by congenital microcephaly, psychomotor retardation, and seizures. Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.

Protein type: Oxidoreductase; Amino Acid Metabolism - glycine, serine and threonine; EC 1.1.1.95; Cell development/differentiation

Chromosomal Location of Human Ortholog: 1p12

Cellular Component: cytosol

Molecular Function: electron carrier activity; NAD binding; phosphoglycerate dehydrogenase activity

Biological Process: neural tube development; glial cell development; glycine metabolic process; gamma-aminobutyric acid metabolic process; glutamine metabolic process; L-serine biosynthetic process; spinal cord development; regulation of gene expression; amino acid biosynthetic process; brain development; taurine metabolic process; neurite development; threonine metabolic process

Disease: Phosphoglycerate Dehydrogenase Deficiency; Neu-laxova Syndrome 1
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PHGDH antibody

 Reference Product  PubMed Publications
 PSPH antibody  >9 publications with PHGDH and PSPH
 PSAT1 antibody  >9 publications with PHGDH and PSAT1
 ASNS antibody  >1 publications with PHGDH and ASNS
 SHMT2 antibody  >1 publications with PHGDH and SHMT2

Pathways associated with anti-PHGDH antibody

 Products by Pathway  Pathway Diagram
 Amino Acid Synthesis And Interconversion (transamination) Pathway antibodies  Amino Acid Synthesis And Interconversion (transamination) Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Glycine, Serine And Threonine Metabolism Pathway antibodies  Glycine, Serine And Threonine Metabolism Pathway Diagram
 Glycine, Serine And Threonine Metabolism Pathway antibodies  Glycine, Serine And Threonine Metabolism Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Amino Acids And Derivatives Pathway antibodies  Metabolism Of Amino Acids And Derivatives Pathway Diagram

Diseases associated with anti-PHGDH antibody

 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >12 publications with PHGDH and Breast Neoplasms
 Melanoma Antibodies  >7 publications with PHGDH and Melanoma
 Nervous System Diseases Antibodies  >7 publications with PHGDH and Nervous System Diseases
 Cell Transformation, Neoplastic Antibodies  >6 publications with PHGDH and Cell Transformation, Neoplastic
 Brain Diseases Antibodies  >5 publications with PHGDH and Brain Diseases
 Disease Models, Animal Antibodies  >4 publications with PHGDH and Disease Models, Animal
 Liver Diseases Antibodies  >3 publications with PHGDH and Liver Diseases
 Phosphoglycerate Dehydrogenase Deficiency Antibodies  >3 publications with PHGDH and Phosphoglycerate Dehydrogenase Deficiency
 Neoplasms, Experimental Antibodies  >3 publications with PHGDH and Neoplasms, Experimental
 Carcinoma, Hepatocellular Antibodies  >1 publications with PHGDH and Carcinoma, Hepatocellular

Organs/Tissues associated with anti-PHGDH antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >20 publications with PHGDH and Brain
 Liver Antibodies  >8 publications with PHGDH and Liver
 Blood Antibodies  >5 publications with PHGDH and Blood
 Lung Antibodies  >4 publications with PHGDH and Lung
 Uterus Antibodies  >2 publications with PHGDH and Uterus
 Nerve Antibodies  >2 publications with PHGDH and Nerve
 Bone Antibodies  >2 publications with PHGDH and Bone
 Embryonic Tissue Antibodies  >2 publications with PHGDH and Embryonic Tissue
 Mammary Gland Antibodies  >1 publications with PHGDH and Mammary Gland
 Muscle Antibodies  >1 publications with PHGDH and Muscle
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