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PHLA2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9600497

Description: This gene is one of several genes in the imprinted gene domain of 11p15.5 which is considered to be an important tumor suppressor gene region. Alterations in this region may be associated with the Beckwith-Wiedemann syndrome, Wilms tumor,… More details

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Product Overview

Online Datasheet in PDF
Product Name
PHLA2, Polyclonal Antibody
Full Product Name
PHLA2 Antibody
Product Gene Name
anti-PHLA2 antibody
Product Synonym Gene Name
PHLDA2
Product Synonym Names
Beckwith Wiedemann syndrome chromosome region 1 candidate protein C; Beckwith-Wiedemann syndrome chromosomal region 1 candidate gene C protein; BRW 1C; BRW1C; BWR 1C; BWR1C; HLDA 2; HLDA2; Imprinted in placenta and liver; Imprinted in placenta and liver protein; IPL; p17 Beckwith Wiedemann region 1C; p17 BWR1C; p17-Beckwith-Wiedemann region 1 C; p17-BWR1C; PHLA2_HUMAN; PHLDA 2; phlda2; Pleckstrin homology like domain family A member 2; Pleckstrin homology-like domain family A member 2; TSSC 3; Tumor suppressing STF cDNA 3 protein; Tumor suppressing subchromosomal transferable fragment candidate gene 3 protein; Tumor suppressing subchromosomal transferable fragment cDNA 3; Tumor suppressing subtransferable candidate 3; Tumor supressing STF cDNA 3; Tumor-suppressing STF cDNA 3 protein; Tumor-suppressing subchromosomal transferable fragment candidate gene 3 protein
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Concentration
1mg/ml (lot specific)
Specificity
PHLA2 antibody detects endogenous levels of PHLA2
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Antibody/Peptide Pairs
PHLA2 peptide (MBS9615050) is used for blocking the activity of PHLA2 antibody (MBS9600497)
Molecular Weight
Observed: 17 kDa
Predicted: 18 kDa
3D Structure
ModBase 3D Structure for Q53GA4
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Immunogen
A synthesized peptide derived from human PHLA2
Subcellular Location
Cytoplasm. Membrane.
Tissue Specificity
Expressed in placenta and adult prostate gland. In placenta, it is present in all cells of the villous cytotrophoblast. The protein is absent in cells from hydatidiform moles. Hydatidiform mole is a gestation characterized by abnormal development of both fetus and trophoblast. The majority of hydatidiform moles are associated with an excess of paternal to maternal genomes and are likely to result from the abnormal expression of imprinted genes (at protein level). Expressed at low levels in adult liver and lung, and fetal liver. Expressed in adult brain and neuroblastoma, medullablastoma and glioblastoma cell lines.
Predicted Cross Reactivity
Pig, Bovine, Chicken
Similarity
Pig (90%), Bovine (89%), Chicken (88%)
Conjugation
Unconjugated
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-PHLA2 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes for anti-PHLA2 antibody
IHC: 1:50-1:200
WB: 1:500-1:2000

Western Blot (WB) of anti-PHLA2 antibody

Western blot analysis of extracts from HepG2, using PHLA2 Antibody. Lane 1 was treated with the antigen-specific peptide.
Rabbit Anti-PHLA2 Polyclonal Antibody – Cat# MBS9600497

Immunohistochemistry (IHC) of anti-PHLA2 antibody

MBS9600497 at 1/100 staining human Breast cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
Rabbit Anti-PHLA2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9600497

Other Notes

Small volumes of anti-PHLA2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PHLA2 purchase

MBS9600497 is an antibody targeting PHLA2. Also known as PHLA2 Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PHLA2. It may not necessarily be applicable to this product.

NCBI GI #
4507705
NCBI GeneID
7262
NCBI Accession #
NP_003302.1
UniProt Primary Accession #
Q53GA4
UniProt Secondary Accession #
O00496
UniProt Related Accession #
Q53GA4
GenBank Nucleotide #
NM_003311.3
NCBI Official Full Name
pleckstrin homology-like domain family A member 2
NCBI Official Synonym Full Names
pleckstrin homology like domain family A member 2
NCBI Official Symbol
PHLDA2
NCBI Official Synonym Symbols
IPL; BRW1C; BWR1C; HLDA2; TSSC3
UniProt Protein Name
Pleckstrin homology-like domain family A member 2
UniProt Synonym Protein Names
Beckwith-Wiedemann syndrome chromosomal region 1 candidate gene C protein; Imprinted in placenta and liver protein; Tumor-suppressing STF cDNA 3 protein; Tumor-suppressing subchromosomal transferable fragment candidate gene 3 protein; p17-Beckwith-Wiedemann region 1 C; p17-BWR1C
UniProt Gene Name
PHLDA2
UniProt Synonym Gene Names
BWR1C; HLDA2; IPL; TSSC3; p17-BWR1C
NCBI Protein Information
pleckstrin homology-like domain family A member 2

NCBI Summary for PHLA2

This gene is located in a cluster of imprinted genes on chromosome 11p15.5, which is considered to be an important tumor suppressor gene region. Alterations in this region may be associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. This gene has been shown to be imprinted, with preferential expression from the maternal allele in placenta and liver. [provided by RefSeq, Oct 2010]
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UniProt Comments for PHLA2

Plays a role in regulating placenta growth. May act via its PH domain that competes with other PH domain-containing proteins, thereby preventing their binding to membrane lipids ().

Research Articles on PHLA2

1. RanBP9/TSSC3 complex cooperatively suppress metastasis via downregulation of Src-dependent Akt pathway to expedite mitochondrial-associated anoikis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-PHLA2 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >8 publications with PHLA2 and Neoplasms
 Liver Diseases Antibodies  >2 publications with PHLA2 and Liver Diseases
 Fetal Death Antibodies  >2 publications with PHLA2 and Fetal Death
 Carcinoma Antibodies  >2 publications with PHLA2 and Carcinoma
 Liver Neoplasms Antibodies  >1 publications with PHLA2 and Liver Neoplasms
 Drug Toxicity Antibodies  >1 publications with PHLA2 and Drug Toxicity
 Kidney Diseases Antibodies  >1 publications with PHLA2 and Kidney Diseases
 Prenatal Exposure Delayed Effects Antibodies  >1 publications with PHLA2 and Prenatal Exposure Delayed Effects
 Cell Transformation, Neoplastic Antibodies  >1 publications with PHLA2 and Cell Transformation, Neoplastic
 Inflammation Antibodies  >1 publications with PHLA2 and Inflammation

Organs/Tissues associated with anti-PHLA2 antibody

 Organ/Tissue Name  Pubmed Publications
 Placenta Antibodies  >21 publications with PHLA2 and Placenta
 Blood Antibodies  >4 publications with PHLA2 and Blood
 Brain Antibodies  >3 publications with PHLA2 and Brain
 Kidney Antibodies  >1 publications with PHLA2 and Kidney
 Skin Antibodies  >1 publications with PHLA2 and Skin
 Prostate Antibodies  >1 publications with PHLA2 and Prostate
 Pancreas Antibodies  >1 publications with PHLA2 and Pancreas
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