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PKM2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9405683

Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio… More details

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Product Overview

Online Datasheet in PDF
Product Name
PKM2, Polyclonal Antibody
Full Product Name
PKM2(phospho-Ser37) Antibody
Product Gene Name
anti-PKM2 antibody
Product Synonym Names
PKM, PK3, OIP3, PK2
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Concentration
1.0 mg/ml (lot specific)
Specificity
The antibody detects endogenous level of PKM2 only when phosphorylated at serine 37.
Preparation and Storage
Store at -20 degree C
Molecular Weight
56,273 Da
OMIM
179050
3D Structure
ModBase 3D Structure for P14618
Immunogen Type
Peptide-KLH
Immunogen Description
Peptide sequence around phosphorylation site of serine 37(I-D-S(p)-P-P) derived from Human PKM2.
Modification
Phospho-Ser37
Target Name
PKM2

Product Categories/Family for anti-PKM2 antibody

Phospho-specific Ab
Applications Tested/Suitable for anti-PKM2 antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes for anti-PKM2 antibody
Western Blot: 1:500~1000

Western Blot (WB) of anti-PKM2 antibody

Western blot analysis of in vitro kinase assays carried out with puried active ERK2, wild-type (WT) PKM2 and PKM2 S37A mutant using PKM2(phospho-Ser37)Antibody.
Rabbit Anti-PKM2 Polyclonal Antibody – Cat# MBS9405683

Immunofluorescence (IF) of anti-PKM2 antibody

Immunofluorescence staining of methanol-fixed MEF cells using PKM2 (phospho-Ser37) Antibody.
Rabbit Anti-PKM2 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS9405683

Other Notes

Small volumes of anti-PKM2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PKM2 purchase

MBS9405683 is an antibody targeting PKM2. Also known as PKM2(phospho-Ser37) Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PKM2. It may not necessarily be applicable to this product.

NCBI GI #
332164775
NCBI GeneID
5315
NCBI Accession #
NP_001193725.1
UniProt Primary Accession #
P14618
UniProt Secondary Accession #
P14786; Q53GK4; Q96E76; Q9BWB5; Q9UCV6; Q9UPF2; A6NFK3; B2R5N8; B3KRY0; B4DFX8; B4DUU6
UniProt Related Accession #
P14618
GenBank Nucleotide #
NM_001206796.1
NCBI Official Full Name
pyruvate kinase PKM isoform c
NCBI Official Synonym Full Names
pyruvate kinase, muscle
NCBI Official Symbol
PKM
NCBI Official Synonym Symbols
PK3; TCB; OIP3; PKM2; CTHBP; THBP1; HEL-S-30
UniProt Protein Name
Pyruvate kinase PKM
UniProt Synonym Protein Names
Cytosolic thyroid hormone-binding protein; CTHBP; Opa-interacting protein 3; OIP-3; Pyruvate kinase 2/3; Pyruvate kinase muscle isozyme; Thyroid hormone-binding protein 1; THBP1; Tumor M2-PK; p58
UniProt Gene Name
PKM
UniProt Synonym Gene Names
OIP3; PK2; PK3; PKM2; CTHBP; OIP-3; THBP1
UniProt Entry Name
KPYM_HUMAN
NCBI Protein Information
pyruvate kinase PKM

NCBI Summary for PKM2

This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported. [provided by RefSeq, May 2011]
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UniProt Comments for PKM2

Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.
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Research Articles on PKM2

1. Results show that PKM2 over-expression in human hepatocellular carcinoma was associated with down-regulation of its regulator mir-122 resulting in an aggressive clinicopathological features and poor prognosis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-PKM2 antibody

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 Adenine Ribonucleotide Biosynthesis, IMP => ADP,ATP Pathway antibodies  Adenine Ribonucleotide Biosynthesis, IMP => ADP,ATP Pathway Diagram
 Adenine Ribonucleotide Biosynthesis, IMP => ADP,ATP Pathway antibodies  Adenine Ribonucleotide Biosynthesis, IMP => ADP,ATP Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Biosynthesis Of Antibiotics Pathway antibodies  Biosynthesis Of Antibiotics Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Central Carbon Metabolism In Cancer Pathway antibodies  Central Carbon Metabolism In Cancer Pathway Diagram
 Central Carbon Metabolism In Cancer Pathway antibodies  Central Carbon Metabolism In Cancer Pathway Diagram
 Glucagon Signaling Pathway antibodies  Glucagon Signaling Pathway Diagram

Diseases associated with anti-PKM2 antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >14 publications with PKM2 and Nervous System Diseases
 Fibrosis Antibodies  >8 publications with PKM2 and Fibrosis
 Lung Diseases Antibodies  >8 publications with PKM2 and Lung Diseases
 Inflammation Antibodies  >7 publications with PKM2 and Inflammation
 Cardiovascular Diseases Antibodies  >7 publications with PKM2 and Cardiovascular Diseases
 Carcinoma Antibodies  >6 publications with PKM2 and Carcinoma
 Cell Transformation, Neoplastic Antibodies  >5 publications with PKM2 and Cell Transformation, Neoplastic
 Necrosis Antibodies  >4 publications with PKM2 and Necrosis
 Liver Diseases Antibodies  >4 publications with PKM2 and Liver Diseases
 Disease Models, Animal Antibodies  >3 publications with PKM2 and Disease Models, Animal

Organs/Tissues associated with anti-PKM2 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >61 publications with PKM2 and Brain
 Blood Antibodies  >42 publications with PKM2 and Blood
 Muscle Antibodies  >28 publications with PKM2 and Muscle
 Kidney Antibodies  >15 publications with PKM2 and Kidney
 Liver Antibodies  >15 publications with PKM2 and Liver
 Heart Antibodies  >11 publications with PKM2 and Heart
 Nerve Antibodies  >10 publications with PKM2 and Nerve
 Vascular Antibodies  >9 publications with PKM2 and Vascular
 Lung Antibodies  >7 publications with PKM2 and Lung
 Thyroid Antibodies  >7 publications with PKM2 and Thyroid
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