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anti-PKM2 antibody :: Rabbit anti-Human PKM2 Polyclonal Antibody

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Catalog # MBS9405683
Unit / Price
  0.05 mL  /  $200 +1 FREE 8GB USB
  0.1 mL  /  $255 +1 FREE 8GB USB
Product Name

PKM2, Polyclonal Antibody

Popular Item
Also Known As

PKM2(phospho-Ser37) Antibody

Product Synonym Names
PKM, PK3, OIP3, PK2
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
605
OMIM
179050
3D Structure
ModBase 3D Structure for P14618
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Specificity
The antibody detects endogenous level of PKM2 only when phosphorylated at serine 37.
Purity/Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Form/Format
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0 mg/ml (lot specific)
Immunogen Type
Peptide-KLH
Immunogen Description
Peptide sequence around phosphorylation site of serine 37(I-D-S(p)-P-P) derived from Human PKM2.
Modification
Phospho-Ser37
Target Name
PKM2
Preparation and Storage
Store at -20 degree C
Other Notes
Small volumes of anti-PKM2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-PKM2 antibody
Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.
Product Categories/Family for anti-PKM2 antibody
Applications Tested/Suitable for anti-PKM2 antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes for anti-PKM2 antibody
Western Blot: 1:500~1000

Western Blot (WB) of anti-PKM2 antibody
Western blot analysis of in vitro kinase assays carried out with puried active ERK2, wild-type (WT) PKM2 and PKM2 S37A mutant using PKM2(phospho-Ser37)Antibody.
anti-PKM2 antibody Western Blot (WB) (WB) image
Immunofluorescence (IF) of anti-PKM2 antibody
Immunofluorescence staining of methanol-fixed MEF cells using PKM2 (phospho-Ser37) Antibody.
anti-PKM2 antibody Immunofluorescence (IF) image
NCBI/Uniprot data below describe general gene information for PKM2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
56,273 Da
NCBI Official Full Name
pyruvate kinase PKM isoform c
NCBI Official Synonym Full Names
pyruvate kinase, muscle
NCBI Official Symbol
NCBI Official Synonym Symbols
PK3; TCB; OIP3; PKM2; CTHBP; THBP1; HEL-S-30
  [Similar Products]
NCBI Protein Information
pyruvate kinase PKM
UniProt Protein Name
Pyruvate kinase PKM
UniProt Synonym Protein Names
Cytosolic thyroid hormone-binding protein; CTHBP; Opa-interacting protein 3; OIP-3; Pyruvate kinase 2/3; Pyruvate kinase muscle isozyme; Thyroid hormone-binding protein 1; THBP1; Tumor M2-PK; p58
UniProt Gene Name
UniProt Synonym Gene Names
OIP3; PK2; PK3; PKM2; CTHBP; OIP-3; THBP1  [Similar Products]
UniProt Entry Name
KPYM_HUMAN
NCBI Summary for PKM2
This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported. [provided by RefSeq, May 2011]
UniProt Comments for PKM2
Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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