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PNMT Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9202041

MBS9202041 is an antibody targeting PNMT. Also known as PNMT Antibody (Center). Reactive in Human. Host: Rabbit. Converts noradrenaline to adrenaline. More details

$240 / 0.08 mL +1 FREE 8GB USB
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Sample Manual Insert View PDF
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
PNMT, Polyclonal Antibody
Full Product Name
PNMT Antibody (Center)
Product Gene Name
Product Synonym Names
Phenylethanolamine N-methyltransferase; PNMTase; Noradrenaline N-methyltransferase; PNMT; PENT
Clonality
Polyclonal
Isotype
Rabbit Ig
Host
Rabbit
Species Reactivity
Human
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Concentration
Vial Concentration: 0.36 (lot specific)
Specificity
This PNMT antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 158-192 amino acids from the Central region of human PNMT.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Antibody/Peptide Pairs
PNMT peptide (MBS9230005) is used for blocking the activity of PNMT antibody (MBS9202041)
Molecular Weight
30855
OMIM
171190
3D Structure
ModBase 3D Structure for P11086
Antigen Source
HUMAN
Applications Tested/Suitable for anti-PNMT antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-PNMT antibody
WB~~1:2000

Western Blot (WB) of anti-PNMT antibody

Anti-PNMT Antibody (Center)at 1:2000 dilution + SW620 whole cell lysates

Lysates/proteins at 20 ug per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

Predicted band size : 31 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Rabbit Anti-PNMT Polyclonal Antibody – Cat# MBS9202041

Product References and Citations for anti-PNMT antibody

Kaneda N.,et al.J. Biol. Chem. 263:7672-7677(1988).
Sasaoka T.,et al.Neurochem. Int. 15:555-565(1989).
Baetge E.E.,et al.Proc. Natl. Acad. Sci. U.S.A. 85:3648-3652(1988).
Martin J.L.,et al.Structure 9:977-985(2001).
Gee C.L.,et al.Biochemistry 44:16875-16885(2005).

Other Notes

Small volumes of anti-PNMT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PNMT. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
phenylethanolamine N-methyltransferase
NCBI Official Synonym Full Names
phenylethanolamine N-methyltransferase
NCBI Official Symbol
PNMT
NCBI Official Synonym Symbols
PENT; PNMTase
UniProt Protein Name
Phenylethanolamine N-methyltransferase
UniProt Synonym Protein Names
Noradrenaline N-methyltransferase
UniProt Gene Name
PNMT
UniProt Synonym Gene Names
PENT; PNMTase
UniProt Entry Name
PNMT_HUMAN
NCBI Protein Information
phenylethanolamine N-methyltransferase

NCBI Summary for PNMT

The product of this gene catalyzes the last step of the catecholamine biosynthesis pathway, which methylates norepinephrine to form epinephrine (adrenaline). The enzyme also has beta-carboline 2N-methyltransferase activity. This gene is thought to play a key step in regulating epinephrine production. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Nov 2012]
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UniProt Comments for PNMT

PNMT: Converts noradrenaline to adrenaline. Belongs to the NNMT/PNMT/TEMT family.

Protein type: Amino Acid Metabolism - tyrosine; EC 2.1.1.28; Methyltransferase

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: cytosol

Molecular Function: phenylethanolamine N-methyltransferase activity

Biological Process: methylation; catecholamine biosynthetic process; epinephrine biosynthetic process

Disease: Hypertension, Essential
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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