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PNPLA6 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1758455

The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring… More details

$210 / 0.01 mg +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
PNPLA6, Polyclonal Antibody
Full Product Name
Anti-PNPLA6 Antibody Picoband
Product Gene Name
anti-PNPLA6 antibody
Product Synonym Names
Acid-sensing ion channel 2; carbonic anhydrase 12; Carbonic anhydrase 12; Carbonate dehydratase XII; Carbonic anhydrase XII; CA-XII; Tumor antigen HOM-RCC-3.1.3; CA12
Clonality
Polyclonal
Isotype
Rabbit IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Purity/Purification
Immunogen affinity purified.
Specificity
HighlyExpressed in colon, kidney, prostate, intestine and activated lymphocytes. Expressed at much higher levels in the renal cell cancers than in surrounding normal kidney tissue. ModeratelyExpressed in pancreas, ovary and testis.Expressed in sweat glands and bronchiolar epithelium (PubMed:26911677).
Preparation and Storage
At -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.
Molecular Weight
149,995 Da
Chromosome Location
Chromosome: 19; NC_000019.9 (7599038..7626653). Location: 19p13.2
OMIM
603197
3D Structure
ModBase 3D Structure for Q8IY17
Immunogen
E coli-derived human PNPLA6 recombinant protein (Position: H15-E1296).
Subcellular Localization
Cell membrane, Membrane, Single-pass type I membrane protein
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500ug/ml.
Cross Reactivity
No cross-reactivity with other proteins.
Protein Function
Reversible hydration of carbon dioxide.

Product Categories/Family for anti-PNPLA6 antibody

Primary Antibodies; Cancer; Cardiovascular; Energy Metabolism; Energy Transfer Pathways; Metabolic Signaling Pathways; Metabolism; Oxidative Stress; Pathways and Processes; Redox Metabolism; Signal Transduction
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-PNPLA6 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)
Application Notes for anti-PNPLA6 antibody
WB: 0.25-0.5ug/ml, Human
ICC/IF: 5ug/ml, Human
FC/FACS (Fixed): 1-3ug/1x1x106 cells, Human
ELISA: 0.1-0.5ug/ml

Western Blot (WB) of anti-PNPLA6 antibody

Figure 1. Western blot analysis of PNPLA6 using anti-PNPLA6 antibody (MBS1758455).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human SH-SY5Y whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PNPLA6 antigen affinity purified polyclonal antibody (#MBS1758455) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (#MBS176460) with Tanon 5200 system. A specific band was detected for PNPLA6 at approximately 150 kDa. The expected band size for PNPLA6 is at 150,151 kDa.
Rabbit Anti-PNPLA6 Polyclonal Antibody – Cat# MBS1758455

Immunofluorescence (IF) of anti-PNPLA6 antibody

Figure 2. IF analysis of PNPLA6 using anti-PNPLA6 antibody (MBS1758455).
PNPLA6 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (#MBS176582) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5ug/mL rabbit anti-PNPLA6 Antibody (MBS1758455) overnight at 4 degree C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Rabbit Anti-PNPLA6 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS1758455

Flow Cytometry (FC/FACS) of anti-PNPLA6 antibody

Figure 3. Flow Cytometry analysis of SiHa cells using anti-PNPLA6 antibody (MBS1758455).
Overlay histogram showing SiHa cells stained with MBS1758455 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PNPLA6 Antibody (MBS1758455, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (MBS177405, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Rabbit Anti-PNPLA6 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1758455

Product References and Citations for anti-PNPLA6 antibody

1. Bargal, R., Avidan, N., Ben-Asher, E., Olender, Z., Zeigler, M., Frumkin, A., Raas-Rothschild, A., Glusman, G., Lancet, D., Bach, G. Identification of the gene causing mucolipidosis type IV. Nature Genet. 26: 118-123, 2000.2. Chalvon-Demersay, A., Tardieu, M., Crosnier, H., Benichou, J. J., Pienkowski, C., Rochiccioli, P., Labrune, B. Syndrome de Laurence-Moon (Bardet-Biedl) avec deficit en hormone de croissance. Arch. Franc. Pediat. 50: 859-862, 1993. 3. Hufnagel, R. B., Arno, G., Hein, N. D., Hersheson, J., Prasad, M., Anderson, Y., Krueger, L. A., Gregory, L. C., Stoetzel, C., Jaworek, T. J., Hull, S., Li, A., and 20 others. Neuropathy target esterase impairments cause Oliver-McFarlane and Laurence-Moon syndromes. J. Med. Genet. 52: 85-94, 2015.

Other Notes

Small volumes of anti-PNPLA6 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PNPLA6 purchase

MBS1758455 is an antibody targeting PNPLA6. Also known as Anti-PNPLA6 Antibody Picoband. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PNPLA6. It may not necessarily be applicable to this product.

NCBI GI #
260656037
NCBI GeneID
10908
NCBI Accession #
NP_001159583.1
UniProt Primary Accession #
Q8IY17
UniProt Secondary Accession #
O60859; Q86W58; Q9UG58; A6NGQ0; B4DFB9; J3KQS3
UniProt Related Accession #
Q8IY17
GenBank Nucleotide #
NM_001166111.1
NCBI Official Full Name
neuropathy target esterase isoform a
NCBI Official Synonym Full Names
patatin-like phospholipase domain containing 6
NCBI Official Symbol
PNPLA6
NCBI Official Synonym Symbols
NTE; sws; SPG39; NTEMND; iPLA2delta
UniProt Protein Name
Neuropathy target esterase
UniProt Synonym Protein Names
Patatin-like phospholipase domain-containing protein 6
Protein Family
Neuropathy target esterase
UniProt Gene Name
PNPLA6
UniProt Synonym Gene Names
NTE
UniProt Entry Name
PLPL6_HUMAN
NCBI Protein Information
neuropathy target esterase; patatin-like phospholipase domain-containing protein 6

NCBI Summary for PNPLA6

This gene encodes a phospholipase that deacetylates intracellular phosphatidylcholine to produce glycerophosphocholine. It is thought to function in neurite outgrowth and process elongation during neuronal differentiation. The protein is anchored to the cytoplasmic face of the endoplasmic reticulum in both neurons and non-neuronal cells. Mutations in this gene result in autosomal recessive spastic paraplegia, and the protein is the target for neurodegeneration induced by organophosphorus compounds and chemical warfare agents. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]
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UniProt Comments for PNPLA6

NTE: Phospholipase B that deacylates intracellular phosphatidylcholine (PtdCho), generating glycerophosphocholine (GroPtdCho). This deacylation occurs at both sn-2 and sn-1 positions of PtdCho. Its specific chemical modification by certain organophosphorus (OP) compounds leads to distal axonopathy. Defects in PNPLA6 are the cause of spastic paraplegia autosomal recessive type 39 (SPG39); also known as NTE-related motor neuron disorder (NTEMND). Spastic paraplegia is a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Rate of progression and the severity of symptoms are quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body. SPG39 is associated with a motor axonopathy affecting upper and lower limbs and resulting in progressive wasting of distal upper and lower extremity muscles. Belongs to the NTE family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; EC 3.1.1.5; Hydrolase

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: endoplasmic reticulum membrane; membrane; endoplasmic reticulum; integral to membrane

Molecular Function: lysophospholipase activity

Biological Process: organ morphogenesis; angiogenesis; developmental process; lipid catabolic process; phosphatidylcholine metabolic process

Disease: Laurence-moon Syndrome; Spastic Paraplegia 39, Autosomal Recessive; Oliver-mcfarlane Syndrome; Boucher-neuhauser Syndrome
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Research Articles on PNPLA6

1. Neuronal phospholipid deacylation is essential for axonal and synaptic integrity through the action of iPLA2 and NTE.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-PNPLA6 antibody

 Products by Pathway  Pathway Diagram
 Glycerophospholipid Metabolism Pathway antibodies  Glycerophospholipid Metabolism Pathway Diagram
 Glycerophospholipid Metabolism Pathway antibodies  Glycerophospholipid Metabolism Pathway Diagram

Diseases associated with anti-PNPLA6 antibody

 Disease Name  Pubmed Publications
 Spastic Paraplegia, Hereditary Antibodies  >3 publications with PNPLA6 and Spastic Paraplegia, Hereditary
 Paralysis Antibodies  >1 publications with PNPLA6 and Paralysis
 Nerve Degeneration Antibodies  >1 publications with PNPLA6 and Nerve Degeneration
 Neurotoxicity Syndromes Antibodies  >1 publications with PNPLA6 and Neurotoxicity Syndromes
 Poisoning Antibodies  >1 publications with PNPLA6 and Poisoning
 Organophosphate Poisoning Antibodies  >1 publications with PNPLA6 and Organophosphate Poisoning
 Motor Neuron Disease Antibodies  >1 publications with PNPLA6 and Motor Neuron Disease
 Nervous System Diseases Antibodies  >1 publications with PNPLA6 and Nervous System Diseases

Organs/Tissues associated with anti-PNPLA6 antibody

 Organ/Tissue Name  Pubmed Publications
 Nerve Antibodies  >1 publications with PNPLA6 and Nerve
 Blood Antibodies  >1 publications with PNPLA6 and Blood
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