Rabbit Anti-PNPO Polyclonal Antibody – Cat# MBS6335435

Western blot analysis of PNPO Antibody (C-term) in 293 cell line lysates (35ug/lane). PNPO (arrow) was detected using the purified Pab.

PNPO Antibody – Rabbit Polyclonal

Name: PNPO Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS6335435
Price: 1060 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6335435

$1060 / 0.2 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

PNPO, Polyclonal Antibody

Full Product Name

PNPO, CT (PNPO, Pyridoxine-5'-phosphate oxidase, Pyridoxamine-phosphate oxidase) (Biotin)

Product Gene Name

anti-PNPO antibody

Product Synonym Names

PNPO; Pyridoxine-5'-phosphate oxidase; Pyridoxamine-phosphate oxidase

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human

Form/Format

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

Purity/Purification

Purified by Protein A Affinity Chromatography.

Preparation and Storage

Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Molecular Weight

25,200 Da

OMIM

603287

3D Structure

ModBase 3D Structure for Q9NVS9

Immunogen

PNPO antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 232~261 amino acids from the C-terminal region of human PNPO.

Conjugate

Biotin

Note

Preservative Free

Product Categories/Family for anti-PNPO antibody

Antibodies; Enzymes; Oxidase

Applications Tested/Suitable for anti-PNPO antibody

Western Blot (WB), ELISA (EIA)

Application Notes for anti-PNPO antibody

WB: 1:100-500
ELISA: 1:1,000
Applications are based on unconjugated antibody.

Product References and Citations for anti-PNPO antibody

Musayev, F.N., et al. J. Biol. Chem. 284(45):30949-30956(2009). Khayat, M., et al. Mol. Genet. Metab. 94(4):431-434(2008). Song, H., et al. Schizophr. Res. 97 (1-3), 264-270 (2007): Mills, P.B., et al. Hum. Mol. Genet. 14(8):1077-1086(2005). Kang, J.H., et al. Eur. J. Biochem. 271(12):2452-2461(2004). Musayev, F.N., et al. Protein Sci. 12(7):1455-1463(2003).

Other Notes

Small volumes of anti-PNPO antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PNPO purchase

MBS6335435 is an antibody targeting PNPO. Also known as PNPO, CT (PNPO, Pyridoxine-5'-phosphate oxidase, Pyridoxamine-phosphate oxidase) (Biotin). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PNPO. It may not necessarily be applicable to this product.

NCBI GI #

8922498

NCBI GeneID

55163

NCBI Accession #

NP_060599.1

UniProt Primary Accession #

Q9NVS9

UniProt Secondary Accession #

B4E0V0; B4E152; B4E1D7; D3DTT9

UniProt Related Accession #

Q9NVS9

GenBank Nucleotide #

NM_018129.3

NCBI Official Full Name

pyridoxine-5'-phosphate oxidase

NCBI Official Synonym Full Names

pyridoxamine 5'-phosphate oxidase

NCBI Official Symbol

PNPO

NCBI Official Synonym Symbols

PDXPO; HEL-S-302

UniProt Protein Name

Pyridoxine-5'-phosphate oxidase

UniProt Synonym Protein Names

Pyridoxamine-phosphate oxidase

Protein Family

Pyridoxine-5'-phosphate oxidase

UniProt Gene Name

PNPO

UniProt Entry Name

PNPO_HUMAN

NCBI Protein Information

pyridoxine-5'-phosphate oxidase; epididymis secretory protein Li 302; pyridoxal 5'-phosphate synthase; pyridoxamine-phosphate oxidase; pyridoxine 5'-phosphate oxidase

NCBI Summary for PNPO

The enzyme encoded by this gene catalyzes the terminal, rate-limiting step in the synthesis of pyridoxal 5'-phosphate, also known as vitamin B6. Vitamin B6 is a required co-factor for enzymes involved in both homocysteine metabolism and synthesis of neurotransmitters such as catecholamine. Mutations in this gene result in pyridoxamine 5'-phosphate oxidase (PNPO) deficiency, a form of neonatal epileptic encephalopathy. [provided by RefSeq, Oct 2008]

UniProt Comments for PNPO

PNPO: Catalyzes the oxidation of either pyridoxine 5'- phosphate (PNP) or pyridoxamine 5'-phosphate (PMP) into pyridoxal 5'-phosphate (PLP). Defects in PNPO are the cause of pyridoxine-5'-phosphate oxidase deficiency (PNPO deficiency); also known as PNPO-related neonatal epileptic encephalopathy. The main feature of neonatal epileptic encephalopathy is the onset within hours of birth of a severe seizure disorder that does not respond to anticonvulsant drugs and can be fatal. Seizures can cease with the administration of PLP, being resistant to treatment with pyridoxine. Belongs to the pyridoxamine 5'-phosphate oxidase family.

Protein type: Cofactor and Vitamin Metabolism - vitamin B6; EC 1.4.3.5; Oxidoreductase

Chromosomal Location of Human Ortholog: 17q21.32

Cellular Component: nucleoplasm; cytoplasm; cytosol

Molecular Function: FMN binding; pyridoxamine-phosphate oxidase activity

Biological Process: vitamin metabolic process; vitamin B6 metabolic process; pyridoxine biosynthetic process; water-soluble vitamin metabolic process; pyridoxal phosphate biosynthetic process

Disease: Pyridoxamine 5-prime-phosphate Oxidase Deficiency

Research Articles on PNPO

1. One sequence variant, R116Q, a single nucleotide polymorphism that has been reported in the general population, was found to have an effect on PNPO activity.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-PNPO antibody

Products by Pathway	Pathway Diagram
Defective AMN Causes Hereditary Megaloblastic Anemia 1 Pathway antibodies	Defective AMN Causes Hereditary Megaloblastic Anemia 1 Pathway Diagram
Defective BTD Causes Biotidinase Deficiency Pathway antibodies	Defective BTD Causes Biotidinase Deficiency Pathway Diagram
Defective CD320 Causes Methylmalonic Aciduria Pathway antibodies	Defective CD320 Causes Methylmalonic Aciduria Pathway Diagram
Defective CUBN Causes Hereditary Megaloblastic Anemia 1 Pathway antibodies	Defective CUBN Causes Hereditary Megaloblastic Anemia 1 Pathway Diagram
Defective GIF Causes Intrinsic Factor Deficiency Pathway antibodies	Defective GIF Causes Intrinsic Factor Deficiency Pathway Diagram
Defective HLCS Causes Multiple Carboxylase Deficiency Pathway antibodies	Defective HLCS Causes Multiple Carboxylase Deficiency Pathway Diagram
Defective LMBRD1 Causes Methylmalonic Aciduria And Homocystinuria Type CblF Pathway antibodies	Defective LMBRD1 Causes Methylmalonic Aciduria And Homocystinuria Type CblF Pathway Diagram
Defective MMAA Causes Methylmalonic Aciduria Type CblA Pathway antibodies	Defective MMAA Causes Methylmalonic Aciduria Type CblA Pathway Diagram
Defective MMAB Causes Methylmalonic Aciduria Type CblB Pathway antibodies	Defective MMAB Causes Methylmalonic Aciduria Type CblB Pathway Diagram
Defective MMACHC Causes Methylmalonic Aciduria And Homocystinuria Type CblC Pathway antibodies	Defective MMACHC Causes Methylmalonic Aciduria And Homocystinuria Type CblC Pathway Diagram

Diseases associated with anti-PNPO antibody

Disease Name	Pubmed Publications
Pyridoxamine 5-Prime-Phosphate Oxidase Deficiency Antibodies	>4 publications with PNPO and Pyridoxamine 5-Prime-Phosphate Oxidase Deficiency
Hypertension Antibodies	>3 publications with PNPO and Hypertension
Fibrosis Antibodies	>2 publications with PNPO and Fibrosis
Liver Diseases Antibodies	>2 publications with PNPO and Liver Diseases
Status Epilepticus Antibodies	>2 publications with PNPO and Status Epilepticus
Infertility, Male Antibodies	>1 publications with PNPO and Infertility, Male
Infertility, Female Antibodies	>1 publications with PNPO and Infertility, Female
Inflammation Antibodies	>1 publications with PNPO and Inflammation
Liver Neoplasms Antibodies	>1 publications with PNPO and Liver Neoplasms
Carcinoma, Hepatocellular Antibodies	>1 publications with PNPO and Carcinoma, Hepatocellular

Organs/Tissues associated with anti-PNPO antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>18 publications with PNPO and Brain
Liver Antibodies	>5 publications with PNPO and Liver
Heart Antibodies	>2 publications with PNPO and Heart
Muscle Antibodies	>2 publications with PNPO and Muscle
Lung Antibodies	>1 publications with PNPO and Lung
Intestine Antibodies	>1 publications with PNPO and Intestine
Eye Antibodies	>1 publications with PNPO and Eye
Ovary Antibodies	>1 publications with PNPO and Ovary
Kidney Antibodies	>1 publications with PNPO and Kidney