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POMT1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6335844

The protein encoded by this gene is an O-mannosyltransferase that requires interaction with the product of the POMT2 gene for enzymatic function. The encoded protein is found in the membrane of the endoplasmic reticulum. Defects in this gene are a cause of… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
POMT1, Polyclonal Antibody
Full Product Name
POMT1, CT (POMT1, Protein O-mannosyl-transferase 1, Dolichyl-phosphate-mannose-protein mannosyltransferase 1) (Azide free) (HRP)
Product Gene Name
Product Synonym Names
POMT1; Protein O-mannosyl-transferase 1; Dolichyl-phosphate-mannose--protein mannosyltransferase 1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
84,881 Da
OMIM
236670
3D Structure
ModBase 3D Structure for Q9Y6A1
Immunogen
POMT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 713-743 amino acids from the C-terminal region of human POMT1.
Conjugate
HRP
Note
Preservative Free

Product Categories/Family for anti-POMT1 antibody

Applications Tested/Suitable for anti-POMT1 antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes for anti-POMT1 antibody
IHC: 1:10-50
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.

Immunohistochemistry (IHC) of anti-POMT1 antibody

POMT1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of POMT1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-POMT1 Polyclonal Antibody – Cat# MBS6335844

Western Blot (WB) of anti-POMT1 antibody

POMT1 Antibody (C-term) western blot analysis in ZR-75-1,K562 cell line lysates (35ug/lane).This demonstrates the POMT1 antibody detected the POMT1 protein (arrow).
Rabbit Anti-POMT1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS6335844

Flow Cytometry (FC/FACS) of anti-POMT1 antibody

POMT1 Antibody (C-term) flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-POMT1 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6335844

Product References and Citations for anti-POMT1 antibody

Manya, H., et al. J. Biochem. 147(3):337-344(2010). Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010): Cotarelo, R.P., et al. Clin. Genet. 76(1):108-112(2009). Mercuri, E., et al. Neurology 72(21):1802-1809(2009). Judas, M., et al. Neuropediatrics 40(1):6-14(2009).

Other Notes

Small volumes of anti-POMT1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for POMT1 purchase

MBS6335844 is an antibody targeting POMT1. Also known as POMT1, CT (POMT1, Protein O-mannosyl-transferase 1, Dolichyl-phosphate-mannose-protein mannosyltransferase 1) (Azide free) (HRP). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for POMT1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
protein O-mannosyl-transferase 1 isoform b
NCBI Official Synonym Full Names
protein-O-mannosyltransferase 1
NCBI Official Symbol
POMT1
NCBI Official Synonym Symbols
RT; LGMD2K; MDDGA1; MDDGB1; MDDGC1
UniProt Protein Name
Protein O-mannosyl-transferase 1
UniProt Synonym Protein Names
Dolichyl-phosphate-mannose--protein mannosyltransferase 1
UniProt Gene Name
POMT1
UniProt Entry Name
POMT1_HUMAN
NCBI Protein Information
protein O-mannosyl-transferase 1; dolichyl-phosphate-mannose--protein mannosyltransferase 1

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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