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PRKACA Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS646795

PRKACA phosphorylates a large number of substrates in the cytoplasm and the nucleus. More details

$895 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PRKACA, Polyclonal Antibody
Full Product Name
PRKACA, NT (K82) (cAMP-dependent Protein Kinase Catalytic Subunit alpha, PKA C-alpha, PKACA)
Product Gene Name
Product Synonym Names
Anti -PRKACA, NT (K82) (cAMP-dependent Protein Kinase Catalytic Subunit alpha, PKA C-alpha, PKACA)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, 0.84% sodium azide.
Purity/Purification
Purified
Purified by ammonium sulfate precipitation.
Specificity
Recognizes human PRKACA.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Chromosome Location
chromosome: 7
Immunogen
Synthetic peptide selected from the N-terminal(K282) region of human PRKACA (KLH).
Applications Tested/Suitable for anti-PRKACA antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-PRKACA antibody
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100

Western Blot (WB) of anti-PRKACA antibody

Western blot analysis of PRKACA Antibody (N-term K82) in NCI-H460 cell line lysates (35ug/lane). PRKACA (arrow) was detected using the purified Pab.
Rabbit Anti-PRKACA Polyclonal Antibody – Cat# MBS646795

Immunohistochemistry (IHC) of anti-PRKACA antibody

Formalin-fixed and paraffin-embedded human testis tissue reacted with PRKACA Antibody (N-term K82), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-PRKACA Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS646795

Flow Cytometry (FC/FACS) of anti-PRKACA antibody

PRKACA Antibody (N-term K82) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-PRKACA Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS646795

Other Notes

Small volumes of anti-PRKACA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PRKACA purchase

MBS646795 is an antibody targeting PRKACA. Also known as PRKACA, NT (K82) (cAMP-dependent Protein Kinase Catalytic Subunit alpha, PKA C-alpha, PKACA). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PRKACA. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
GenBank Nucleotide #
NCBI Official Full Name
cAMP-dependent protein kinase catalytic subunit alpha
NCBI Official Symbol
PRKACA
NCBI Protein Information
protein kinase, cAMP-dependent, catalytic, alpha

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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