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PRMT6 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1758348

The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring… More details

$210 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
PRMT6, Polyclonal Antibody
Full Product Name
Anti-PRMT6 Antibody Picoband
Product Gene Name
anti-PRMT6 antibody
Product Synonym Names
Peroxisomal multifunctional enzyme type 2; Peroxisomal multifunctional enzyme type 2; MFE-2; 17-beta-hydroxysteroid dehydrogenase 4; 17-beta-HSD 4; D-bifunctional protein; DBP; Multifunctional protein 2; MPF-2; Short chain dehydrogenase/reductase family 8C member 1; (3R)-hydroxyacyl-CoA dehydrogenase; 1.1.1.n12; Enoyl-CoA hydratase 2; 4.2.1.107; 4.2.1.119; 3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholest-24-enoyl-CoA hydratase; HSD17B4; EDH17B4, SDR8C1
Clonality
Polyclonal
Isotype
Rabbit IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Purity/Purification
Immunogen affinity purified.
Specificity
Present in many tissues with highest concentrations in liver, heart, prostate and testis.
Preparation and Storage
At -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.
Molecular Weight
375
OMIM
608274
3D Structure
ModBase 3D Structure for Q96LA8
Immunogen
E coli-derived human PRMT6 recombinant protein (Position: Q45-D348).
Subcellular Localization
Peroxisome
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500ug/ml.
Cross Reactivity
No cross-reactivity with other proteins
Protein Function
Bifunctional enzyme acting on the peroxisomal beta- oxidation pathway for fatty acids. Catalyzes the formation of 3- ketoacyl-CoA intermediates from both straight-chain and 2-methyl- branched-chain fatty acids.

Product Categories/Family for anti-PRMT6 antibody

Primary Antibodies; Cancer; Cardiovascular; Fatty Acid Oxidation; Fatty Acids; Lipid and Lipoprotein Metabolism; Lipid Metabolism; Lipids/Lipoproteins; Metabolic Signaling Pathways; Metabolism; Pathways and Processes; Redox Metabolism; Signal Transduction
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-PRMT6 antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes for anti-PRMT6 antibody
WB: 0.25-0.5ug/ml, Human
FC/FACS (Fixed): 1-3ug /1x106 cells, Human
ELISA: 0.1-0.5ug/ml

Western Blot (WB) of anti-PRMT6 antibody

Figure 1. Western blot analysis of PRMT6 using anti-PRMT6 antibody (MBS1758348).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRMT6 antigen affinity purified polyclonal antibody (#MBS1758348) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (#MBS176460) with Tanon 5200 system. A specific band was detected for PRMT6 at approximately 40 kDa. The expected band size for PRMT6 is at 40 kDa.
Rabbit Anti-PRMT6 Polyclonal Antibody – Cat# MBS1758348

Flow Cytometry (FC/FACS) of anti-PRMT6 antibody

Figure 2. Flow Cytometry analysis of SH-SY5Y cells using anti-PRMT6 antibody (MBS1758348).
Overlay histogram showing SH-SY5Y cells stained with MBS1758348 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PRMT6 Antibody (MBS1758348, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (MBS177405, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Rabbit Anti-PRMT6 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1758348

Product References and Citations for anti-PRMT6 antibody

1. El-Andaloussi, N., Valovka, T., Toueille, M., Steinacher, R., Focke, F., Gehrig, P., Covic, M., Hassa, P. O., Schar, P., Hubscher, U., Hottiger, M. O. Arginine methylation regulates DNA polymerase beta. Molec. Cell 22: 51-62, 2006.2. Frankel, A., Yadav, N., Lee, J., Branscombe, T. L., Clarke, S., Bedford, M. T. The novel human protein arginine N-methyltransferase PRMT6 is a nuclear enzyme displaying unique substrate specificity. J. Biol. Chem. 277: 3537-3543, 2002. 3. Guccione, E., Bassi, C., Casadio, F., Martinato, F., Cesaroni, M., Schuchlautz, H., Luscher, B., Amati, B. Methylation of histone H3R2 by PRMT6 and H3K4 by an MLL complex are mutually exclusive. Nature 449: 933-937, 2007.

Other Notes

Small volumes of anti-PRMT6 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PRMT6 purchase

MBS1758348 is an antibody targeting PRMT6. Also known as Anti-PRMT6 Antibody Picoband. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PRMT6. It may not necessarily be applicable to this product.

NCBI GI #
20137409
NCBI GeneID
55170
NCBI Accession #
Q96LA8.1
UniProt Primary Accession #
Q96LA8
UniProt Secondary Accession #
Q5T5Y5; Q6DKI4; Q9NVR8; A3KME1; B4DID8
UniProt Related Accession #
Q96LA8
NCBI Official Full Name
Protein arginine N-methyltransferase 6
NCBI Official Synonym Full Names
protein arginine methyltransferase 6
NCBI Official Symbol
PRMT6
NCBI Official Synonym Symbols
HRMT1L6
UniProt Protein Name
Protein arginine N-methyltransferase 6
UniProt Synonym Protein Names
Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 6; Histone-arginine N-methyltransferase PRMT6 (EC:2.1.1.125)
Protein Family
Probable protein arginine N-methyltransferase
UniProt Gene Name
PRMT6
UniProt Synonym Gene Names
HRMT1L6
UniProt Entry Name
ANM6_HUMAN
NCBI Protein Information
protein arginine N-methyltransferase 6; HMT1 hnRNP methyltransferase-like 6; histone-arginine N-methyltransferase PRMT6; heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 6

NCBI Summary for PRMT6

The protein encoded by this gene belongs to the arginine N-methyltransferase family, which catalyze the sequential transfer of methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins, to form methylated arginine derivatives and S-adenosyl-L-homocysteine. This protein can catalyze both, the formation of omega-N monomethylarginine and asymmetrical dimethylarginine, with a strong preference for the latter. It specifically mediates the asymmetric dimethylation of Arg2 of histone H3, and the methylated form represents a specific tag for epigenetic transcriptional repression. This protein also forms a complex with, and methylates DNA polymerase beta, resulting in stimulation of polymerase activity by enhancing DNA binding and processivity. [provided by RefSeq, Sep 2011]
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UniProt Comments for PRMT6

PRMT6: Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and asymmetrical dimethylarginine (aDMA), with a strong preference for the formation of aDMA. Preferentially methylates arginyl residues present in a glycine and arginine-rich domain and displays preference for monomethylated substrates. Specifically mediates the asymmetric dimethylation of histone H3 'Arg-2' to form H3R2me2a. H3R2me2a represents a specific tag for epigenetic transcriptional repression and is mutually exclusive with methylation on histone H3 'Lys-4' (H3K4me2 and H3K4me3). It thereby acts as a transcription corepressor of various genes such as HOXA2. Also methylates histone H2A and H4 'Arg-3' (H2AR3me and H4R3me, respectively). Acts as a regulator of DNA base excision during DNA repair by mediating the methylation of DNA polymerase beta (POLB), leading to stimulate the polymerase activity by enhancing DNA binding and processivity. Methylates HMGA1. May play a role in innate immunity against HIV-1 in case of infection by methylating and impairing the function of various HIV-1 proteins such as Tat, Rev and Nucleocapsid protein p7 (NC). Belongs to the protein arginine N-methyltransferase family. PRMT6 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Methyltransferase; EC 2.1.1.125; Methyltransferase, protein arginine

Chromosomal Location of Human Ortholog: 1p13.3

Cellular Component: nucleoplasm; nucleus; cytosol

Molecular Function: histone methyltransferase activity; protein-arginine omega-N asymmetric methyltransferase activity; protein-arginine omega-N monomethyltransferase activity; protein binding; histone binding

Biological Process: histone methylation; establishment and/or maintenance of chromatin architecture; viral reproduction; transcription, DNA-dependent; base-excision repair; peptidyl-arginine methylation, to asymmetrical-dimethyl arginine; negative regulation of transcription, DNA-dependent
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Research Articles on PRMT6

1. PRMT6 specifically interacts with ERalpha at its ligand-binding domain.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PRMT6 antibody

 Reference Product  PubMed Publications
 HMGA1 antibody  >4 publications with PRMT6 and HMGA1

Pathways associated with anti-PRMT6 antibody

 Products by Pathway  Pathway Diagram
 Chromatin Modifying Enzymes Pathway antibodies  Chromatin Modifying Enzymes Pathway Diagram
 Chromatin Organization Pathway antibodies  Chromatin Organization Pathway Diagram
 RMTs Methylate Histone Arginines Pathway antibodies  RMTs Methylate Histone Arginines Pathway Diagram

Diseases associated with anti-PRMT6 antibody

 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >5 publications with PRMT6 and Breast Neoplasms
 Infertility, Male Antibodies  >4 publications with PRMT6 and Infertility, Male
 Liver Neoplasms Antibodies  >2 publications with PRMT6 and Liver Neoplasms
 Death Antibodies  >2 publications with PRMT6 and Death
 Inflammation Antibodies  >2 publications with PRMT6 and Inflammation
 Hypertension Antibodies  >1 publications with PRMT6 and Hypertension
 Liver Diseases Antibodies  >1 publications with PRMT6 and Liver Diseases
 Atrophy Antibodies  >1 publications with PRMT6 and Atrophy
 Carcinoma, Hepatocellular Antibodies  >1 publications with PRMT6 and Carcinoma, Hepatocellular
 Parkinson Disease Antibodies  >1 publications with PRMT6 and Parkinson Disease

Organs/Tissues associated with anti-PRMT6 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >5 publications with PRMT6 and Blood
 Prostate Antibodies  >3 publications with PRMT6 and Prostate
 Liver Antibodies  >3 publications with PRMT6 and Liver
 Spleen Antibodies  >3 publications with PRMT6 and Spleen
 Lung Antibodies  >3 publications with PRMT6 and Lung
 Brain Antibodies  >3 publications with PRMT6 and Brain
 Muscle Antibodies  >2 publications with PRMT6 and Muscle
 Bladder Antibodies  >2 publications with PRMT6 and Bladder
 Kidney Antibodies  >1 publications with PRMT6 and Kidney
 Intestine Antibodies  >1 publications with PRMT6 and Intestine
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