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PTPN13 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1758305

The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring… More details

$210 / 0.01 mg +1 FREE 8GB USB
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Sample Manual Insert View PDF
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Product Overview

Online Datasheet in PDF
Product Name
PTPN13, Polyclonal Antibody
Full Product Name
Anti-PTPN13 Antibody Picoband
Product Gene Name
anti-PTPN13 antibody
Product Synonym Names
fatty acid binding protein 1; Fatty acid-binding protein, liver; Fatty acid-binding protein 1; Liver-type fatty acid-binding protein; L-FABP; Squalene- and sterol-carrier protein; SCP; Z-protein; p14; Fabp1
Clonality
Polyclonal
Isotype
Rabbit IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Purity/Purification
Immunogen affinity purified.
Specificity
Expressed in lung cancers, including adenocarcinoma, squamous cell carcinoma and small-cell carcinoma.Widely expressed.Expressed in fetal kidney, liver, lung and brain. In adult highest expression in heart and placenta.
Preparation and Storage
At -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.
Molecular Weight
277,508 Da
OMIM
600267
3D Structure
ModBase 3D Structure for Q12923
Immunogen
E coli-derived human PTPN13 recombinant protein (Position: E1795-D2046).
Subcellular Localization
Cytoplasm
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500ug/ml.
Cross Reactivity
No cross-reactivity with other proteins.
Protein Function
Plays a role in lipoprotein-mediated cholesterol uptake in hepatocytes (By similarity). Binds cholesterol (PubMed:25732850). Binds free fatty acids and their coenzyme A derivatives, bilirubin, and some other small molecules in the cytoplasm. May be involved in intracellular lipid transport (By similarity).

Product Categories/Family for anti-PTPN13 antibody

Primary Antibodies; Fatty Acid Oxidation; Fatty Acids; Lipid and Lipoprotein Metabolism; Lipid Metabolism; Metabolic Signaling Pathways; Metabolism; Pathways and Processes; Redox Metabolism; Signal Transduction
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-PTPN13 antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes for anti-PTPN13 antibody
WB: 0.25-0.5ug/ml, Human
FC/FACS (Fixed): 1-3ug /1x106 cells, Human
ELISA: 0.1-0.5ug/ml

Western Blot (WB) of anti-PTPN13 antibody

Figure 1. Western blot analysis of PTPN13 using anti-PTPN13 antibody (MBS1758305).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human RT4 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTPN13 antigen affinity purified polyclonal antibody (#MBS1758305) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (#MBS176460) with Tanon 5200 system. A specific band was detected for PTPN13 at approximately 270 kDa. The expected band size for PTPN13 is at 270 kDa.
Rabbit Anti-PTPN13 Polyclonal Antibody – Cat# MBS1758305

Flow Cytometry (FC/FACS) of anti-PTPN13 antibody

Figure 2. Flow Cytometry analysis of RT4 cells using anti-PTPN13 antibody (MBS1758305).
Overlay histogram showing RT4 cells stained with MBS1758305 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PTPN13 Antibody (MBS1758305, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (MBS177405, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Rabbit Anti-PTPN13 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1758305

Product References and Citations for anti-PTPN13 antibody

1. Banville, D., Ahmad, S., Stocco, R., Shen, S.-H. A novel protein-tyrosine phosphatase with homology to both the cytoskeletal proteins of the band 4.1 family and junction-associated guanylate kinases. J. Biol. Chem. 269: 22320-22327, 1994. Note: Erratum: J. Biol. Chem. 270: 10359 only, 1995.2. Huang, W., Zhu, C., Wang, H., Horvath, E., Eklund, E. A. The interferon consensus sequence-binding protein (ICSBP/IRF8) represses PTPN13 gene transcription in differentiating myeloid cells. J. Biol. Chem. 283: 7921-7935, 2008.3. Inazawa, J., Ariyama, T., Abe, T., Druck, T., Ohta, M., Huebner, K., Yanagisawa, J., Reed, J. C., Sato, T. PTPN13, a Fas-associated protein tyrosine phosphatase, is located on the long arm of chromosome 4 at band q21.3. Genomics 31: 240-242, 1996.

Other Notes

Small volumes of anti-PTPN13 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PTPN13 purchase

MBS1758305 is an antibody targeting PTPN13. Also known as Anti-PTPN13 Antibody Picoband. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PTPN13. It may not necessarily be applicable to this product.

NCBI GI #
5453992
NCBI GeneID
5783
NCBI Accession #
NP_006255.1
UniProt Primary Accession #
Q12923
UniProt Secondary Accession #
Q15159; Q15263; Q15264; Q15265; Q15674; Q16826; Q8IWH7; Q9NYN9; Q9UDA8; B2RTR0
UniProt Related Accession #
Q12923
GenBank Nucleotide #
NM_006264.2
NCBI Official Full Name
tyrosine-protein phosphatase non-receptor type 13 isoform 2
NCBI Official Synonym Full Names
protein tyrosine phosphatase, non-receptor type 13 (APO-1/CD95 (Fas)-associated phosphatase)
NCBI Official Symbol
PTPN13
NCBI Official Synonym Symbols
PNP1; FAP-1; PTP1E; PTPL1; PTPLE; PTP-BL; hPTP1E; PTP-BAS
UniProt Protein Name
Tyrosine-protein phosphatase non-receptor type 13
UniProt Synonym Protein Names
Fas-associated protein-tyrosine phosphatase 1; FAP-1; PTP-BAS; Protein-tyrosine phosphatase 1E; PTP-E1; hPTPE1; Protein-tyrosine phosphatase PTPL1
Protein Family
Tyrosine-protein phosphatase non-receptor
UniProt Gene Name
PTPN13
UniProt Synonym Gene Names
PNP1; PTP1E; PTPL1; FAP-1; PTP-E1; hPTPE1
UniProt Entry Name
PTN13_HUMAN
NCBI Protein Information
tyrosine-protein phosphatase non-receptor type 13; hPTPE1; protein-tyrosine phosphatase 1E; protein-tyrosine phosphatase PTPL1; fas-associated protein-tyrosine phosphatase 1

NCBI Summary for PTPN13

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP is a large intracellular protein. It has a catalytic PTP domain at its C-terminus and two major structural domains: a region with five PDZ domains and a FERM domain that binds to plasma membrane and cytoskeletal elements. This PTP was found to interact with, and dephosphorylate, Fas receptor and IkappaBalpha through the PDZ domains. This suggests it has a role in Fas mediated programmed cell death. This PTP was also shown to interact with GTPase-activating protein, and thus may function as a regulator of Rho signaling pathways. Four alternatively spliced transcript variants, which encode distinct proteins, have been reported. [provided by RefSeq, Oct 2008]
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UniProt Comments for PTPN13

PTPN13: Tyrosine phosphatase which regulates negatively FAS- induced apoptosis and NGFR-mediated pro-apoptotic signaling. Belongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; EC 3.1.3.48; Protein phosphatase, tyrosine (non-receptor)

Chromosomal Location of Human Ortholog: 4q21.3

Cellular Component: neuron projection; cytoskeleton; lamellipodium; cytoplasm; plasma membrane; nucleus

Molecular Function: protein binding; protein tyrosine phosphatase activity

Biological Process: regulation of phosphoinositide 3-kinase cascade; protein amino acid dephosphorylation
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Research Articles on PTPN13

1. Thus, our results suggest a previously unknown Stat3-PTPN13 molecular network controlling squamous cell lung carcinoma development

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-PTPN13 antibody

 Products by Pathway  Pathway Diagram
 Apoptosis Modulation And Signaling Pathway antibodies  Apoptosis Modulation And Signaling Pathway Diagram
 Ephrin B Reverse Signaling Pathway antibodies  Ephrin B Reverse Signaling Pathway Diagram
 Fc-epsilon Receptor I Signaling In Mast Cells Pathway antibodies  Fc-epsilon Receptor I Signaling In Mast Cells Pathway Diagram

Diseases associated with anti-PTPN13 antibody

 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >34 publications with PTPN13 and Carcinoma
 Adenocarcinoma Antibodies  >20 publications with PTPN13 and Adenocarcinoma
 Colorectal Neoplasms Antibodies  >15 publications with PTPN13 and Colorectal Neoplasms
 Breast Neoplasms Antibodies  >15 publications with PTPN13 and Breast Neoplasms
 Necrosis Antibodies  >14 publications with PTPN13 and Necrosis
 Carcinoma, Squamous Cell Antibodies  >9 publications with PTPN13 and Carcinoma, Squamous Cell
 Lung Neoplasms Antibodies  >8 publications with PTPN13 and Lung Neoplasms
 Liver Diseases Antibodies  >7 publications with PTPN13 and Liver Diseases
 Pancreatic Neoplasms Antibodies  >6 publications with PTPN13 and Pancreatic Neoplasms
 Liver Neoplasms Antibodies  >5 publications with PTPN13 and Liver Neoplasms

Organs/Tissues associated with anti-PTPN13 antibody

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >9 publications with PTPN13 and Lung
 Brain Antibodies  >9 publications with PTPN13 and Brain
 Bone Antibodies  >7 publications with PTPN13 and Bone
 Thyroid Antibodies  >6 publications with PTPN13 and Thyroid
 Kidney Antibodies  >4 publications with PTPN13 and Kidney
 Testis Antibodies  >4 publications with PTPN13 and Testis
 Mouth Antibodies  >2 publications with PTPN13 and Mouth
 Prostate Antibodies  >2 publications with PTPN13 and Prostate
 Eye Antibodies  >2 publications with PTPN13 and Eye
 Embryonic Tissue Antibodies  >2 publications with PTPN13 and Embryonic Tissue
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