Rabbit Anti-PUDP Polyclonal Antibody – Cat# MBS1759346

Figure 1. Western blot analysis of PUDP using anti-PUDP antibody (MBS1759346).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human Caco-2 whole cell lysates,
Lane 4: human A431 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PUDP antigen affinity purified polyclonal antibody (#MBS1759346) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (#MBS176460) with Tanon 5200 system. A specific band was detected for PUDP at approximately 25 kDa. The expected band size for PUDP is at 25 kDa.

PUDP Antibody – Rabbit Polyclonal

Name: PUDP Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS1759346
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1759346

The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring… More details

$210 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

PUDP, Polyclonal Antibody

Full Product Name

Anti-PUDP Antibody Picoband

Product Gene Name

anti-PUDP antibody

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human

Form/Format

Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purity/Purification

Immunogen affinity purified.

Preparation and Storage

At -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.

Molecular Weight

27,573 Da

OMIM

306480

3D Structure

ModBase 3D Structure for Q08623

Immunogen

E coli-derived human PUDP recombinant protein (Position: M1-E228). Human PUDP shares 71.2% amino acid (aa) sequence identity with mouse PUDP.

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500ug/ml.

Product Categories/Family for anti-PUDP antibody

Primary Antibodies

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-PUDP antibody

Western Blot (WB), ELISA (EIA)

Application Notes for anti-PUDP antibody

WB: 0.25-0.5ug/ml, Human
ELISA: 0.1-0.5ug/ml

Product References and Citations for anti-PUDP antibody

1. Yen, P. H., Ellison, J., Salido, E. C., Mohandas, T., Shapiro, L. Isolation of a new gene from the distal short arm of the human X chromosome that escapes X-inactivation. Hum. Molec. Genet. 1: 47-52, 1992.

Other Notes

Small volumes of anti-PUDP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PUDP purchase

MBS1759346 is an antibody targeting PUDP. Also known as Anti-PUDP Antibody Picoband. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PUDP. It may not necessarily be applicable to this product.

NCBI GI #

207113149

NCBI GeneID

8226

NCBI Accession #

NP_001129037.1

UniProt Primary Accession #

Q08623

UniProt Secondary Accession #

Q53F84; Q96EB8; B2R7X6; B4DV93; B7Z6Q3; E9PAV8; F5GWZ2

UniProt Related Accession #

Q08623

GenBank Nucleotide #

NM_001135565.1

NCBI Official Full Name

pseudouridine-5'-monophosphatase isoform a

NCBI Official Synonym Full Names

haloacid dehalogenase-like hydrolase domain containing 1

NCBI Official Symbol

HDHD1

NCBI Official Synonym Symbols

GS1; HDHD1A; FAM16AX; DXF68S1E

UniProt Protein Name

Pseudouridine-5'-monophosphatase

UniProt Synonym Protein Names

Haloacid dehalogenase-like hydrolase domain-containing protein 1; Haloacid dehalogenase-like hydrolase domain-containing protein 1A; Protein GS1

Protein Family

Pseudouridine-5'-phosphatase

UniProt Gene Name

HDHD1

UniProt Synonym Gene Names

DXF68S1E; FAM16AX; GS1; HDHD1A; 5'-PsiMPase

UniProt Entry Name

HDHD1_HUMAN

NCBI Protein Information

pseudouridine-5'-monophosphatase; 5'-PsiMPase; family with sequence similarity 16, member A, X-linked; haloacid dehalogenase-like hydrolase domain containing 1A; haloacid dehalogenase-like hydrolase domain-containing protein 1; haloacid dehalogenase-like

NCBI Summary for PUDP

This gene encodes a member of the haloacid dehalogenase-like (HAD) hydrolase superfamily. The encoded protein has no known biological function. This gene has a pseudogene on chromosome 1. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2010]

UniProt Comments for PUDP

HDHD1A: Dephosphorylates pseudouridine 5'-phosphate, a potential intermediate in rRNA degradation. Pseudouridine is then excreted intact in urine. Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.3.n6

Chromosomal Location of Human Ortholog: Xp22.32

Molecular Function: hydrolase activity; metal ion binding

Biological Process: nucleotide metabolic process

Research Articles on PUDP

1. HDHD1 is a phosphatase specifically involved in dephosphorylation of a modified nucleotide present in RNA.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-PUDP antibody

Disease Name	Pubmed Publications
Skin Diseases Antibodies	>1 publications with PUDP and Skin Diseases

Organs/Tissues associated with anti-PUDP antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>2 publications with PUDP and Blood
Brain Antibodies	>1 publications with PUDP and Brain