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RAB27B Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6339668

MBS6339668 is an antibody targeting RAB27B. Also known as RAB27B, CT (RAB27B, Ras-related protein Rab-27B, C25KG) (AP). Reactive in Human. Host: Rabbit. RAB27B may be involved in targeting uroplakins to urothelial apical membranes. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
RAB27B, Polyclonal Antibody
Full Product Name
RAB27B, CT (RAB27B, Ras-related protein Rab-27B, C25KG) (AP)
Product Gene Name
Product Synonym Names
RAB27B; Ras-related protein Rab-27B; C25KG
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
26.7 kDa (238aa), confirmed by MALDI-TOF
OMIM
603869
3D Structure
ModBase 3D Structure for O00194
Immunogen
RAB27B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between amino acid 188-218 from the C-terminal region of human RAB27B.
Conjugate
AP
Note
Preservative Free

Product Categories/Family for anti-RAB27B antibody

Applications Tested/Suitable for anti-RAB27B antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-RAB27B antibody
WB: 1:100-500
ELISA: 1:1,000
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-RAB27B antibody

Western blot analysis of RAB27B Antibody (C-term) in NCI-H460 cell line lysates (35ug/lane). RAB27B (arrow) was detected using the purified Pab.
Rabbit Anti-RAB27B Polyclonal Antibody – Cat# MBS6339668

Flow Cytometry (FC/FACS) of anti-RAB27B antibody

RAB27B Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-RAB27B Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6339668

Product References and Citations for anti-RAB27B antibody

Hayasaka, S., et al. Asian J. Androl. 10(4):561-568(2008). Shirakawa, R., et al. J. Biol. Chem. 279(11):10730-10737(2004). Tiwari, S., et al. Blood 102(12):3970-3979(2003).

Other Notes

Small volumes of anti-RAB27B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for RAB27B. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
ras-related protein Rab-27B
NCBI Official Synonym Full Names
RAB27B, member RAS oncogene family
NCBI Official Symbol
RAB27B
NCBI Official Synonym Symbols
C25KG
UniProt Protein Name
Ras-related protein Rab-27B
UniProt Synonym Protein Names
C25KG
Protein Family
UniProt Gene Name
RAB27B
UniProt Entry Name
RB27B_HUMAN
NCBI Protein Information
ras-related protein Rab-27B

NCBI Summary for RAB27B

Members of the Rab protein family, including RAB27B, are prenylated, membrane-bound proteins involved in vesicular fusion and trafficking (Chen et al., 1997 [PubMed 9066979]).[supplied by OMIM, Nov 2010]

UniProt Comments for RAB27B

RAB27B: May be involved in targeting uroplakins to urothelial apical membranes. Belongs to the small GTPase superfamily. Rab family.

Protein type: G protein, monomeric; G protein, monomeric, Rab

Chromosomal Location of Human Ortholog: 18q21.2

Cellular Component: trans-Golgi network transport vesicle; zymogen granule membrane; Golgi stack; apical plasma membrane; melanosome; secretory granule

Molecular Function: GTPase activity; protein domain specific binding; protein binding; GDP binding; GTP binding; myosin V binding

Biological Process: intracellular protein transport; metabolic process; protein secretion; Rab protein signal transduction; melanosome transport; vesicle docking during exocytosis; positive regulation of exocytosis
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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