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RAD9 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6496464

MBS6496464 is an antibody targeting Rad9. Also known as Rad9, Phosphorylated (Ser277) (RAD9A, Cell Cycle Checkpoint Control Protein RAD9A, hRAD9, DNA Repair Exonuclease Rad9 Homolog A) (APC). Reactive in Human. Host: Rabbit. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Rad9, Polyclonal Antibody
Full Product Name
Rad9, Phosphorylated (Ser277) (RAD9A, Cell Cycle Checkpoint Control Protein RAD9A, hRAD9, DNA Repair Exonuclease Rad9 Homolog A) (APC)
Product Gene Name
anti-Rad9 antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Purity/Purification
Purified by Immunoaffinity chromatography.
Specificity
Recognizes human Rad9 when phosphorylated at Ser277.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze APC conjugates. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
42,547 Da
OMIM
603761
3D Structure
ModBase 3D Structure for Q99638
Immunogen
Synthetic phosphopeptide corresponding to amino acid residues surrounding Ser277 of human Rad9 (KLH).
Conjugate
APC
Antigen Modification
Phosphorylated
Note
Preservative Free
Special Handling
Light sensitive

Product Categories/Family for anti-Rad9 antibody

Antibodies; DNA Replication; Repair
Applications Tested/Suitable for anti-Rad9 antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes for anti-Rad9 antibody
IHC: 1:50-1:100
FLISA: 1:1,000
WB: 1:100-1:500
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-Rad9 antibody

The anti-Phospho-Rad9-S277 Pab is used in Western blot to detect Phospho-Rad9-S277 in Y79 tissue lysate
Rabbit Anti-RAD9 Polyclonal Antibody – Cat# MBS6496464

Immunohistochemistry (IHC) of anti-Rad9 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Rabbit Anti-RAD9 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6496464

Product References and Citations for anti-Rad9 antibody

1. Maniwa, Y., et al., Cancer 103(1):126-132 (2005). 2. Wang, W., et al., Proc. Natl. Acad. Sci. USA 101(48):16762-16767 (2004). 3. Lindsey-Boltz, L.A., et al., (er) Nucleic Acids Res. 32(15):4524-4530 (2004). 4. Toueille, M., et al., (er) Nucleic Acids Res. 32(11):3316-3324 (2004). 5. Loegering, D., et al., J. Biol. Chem. 279(18):18641-18647 (2004).

Other Notes

Small volumes of anti-Rad9 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for Rad9. It may not necessarily be applicable to this product.

NCBI GI #
4759022
NCBI GeneID
5883
NCBI Accession #
NP_004575
UniProt Primary Accession #
Q99638
UniProt Secondary Accession #
Q6FI29; Q96C41; B2RCZ8
UniProt Related Accession #
Q99638
GenBank Nucleotide #
NM_004584
NCBI Official Full Name
cell cycle checkpoint control protein RAD9A isoform 1
NCBI Official Synonym Full Names
RAD9 homolog A (S. pombe)
NCBI Official Symbol
RAD9A
NCBI Official Synonym Symbols
RAD9
UniProt Protein Name
Cell cycle checkpoint control protein RAD9A
UniProt Synonym Protein Names
DNA repair exonuclease rad9 homolog A
Protein Family
DNA repair protein
UniProt Gene Name
RAD9A
UniProt Synonym Gene Names
hRAD9
UniProt Entry Name
RAD9A_HUMAN
NCBI Protein Information
cell cycle checkpoint control protein RAD9A; DNA repair exonuclease rad9 homolog A; hRAD9

UniProt Comments for Rad9

Rad9: a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair in response to DNA damage. Possesses a 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. Forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Use of alternative polyA sites has been noted for Rad9.

Protein type: EC 3.1.11.2; DNA replication; Deoxyribonuclease; Apoptosis; Cell cycle regulation

Chromosomal Location of Human Ortholog: 11q13.1-q13.2

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: protein binding; enzyme binding; histone deacetylase binding; exodeoxyribonuclease III activity; 3'-5' exonuclease activity; SH3 domain binding; protein kinase binding

Biological Process: intra-S DNA damage checkpoint; DNA damage checkpoint; DNA replication checkpoint; DNA replication; DNA repair; DNA catabolic process, exonucleolytic; response to DNA damage stimulus
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Rad9 antibody

 Reference Product  PubMed Publications
 TOPBP1 antibody  >5 publications with Rad9 and TOPBP1
 RAD9B antibody  >3 publications with Rad9 and RAD9B
 RAD17 antibody  >2 publications with Rad9 and RAD17
 HUS1 antibody  >2 publications with Rad9 and HUS1
 HUS1B antibody  >1 publications with Rad9 and HUS1B
 ATM antibody  >1 publications with Rad9 and ATM
 CLSPN antibody  >1 publications with Rad9 and CLSPN

Pathways associated with anti-Rad9 antibody

 Products by Pathway  Pathway Diagram
 Activation Of ATR In Response To Replication Stress Pathway antibodies  Activation Of ATR In Response To Replication Stress Pathway Diagram
 Androgen Receptor Signaling Pathway antibodies  Androgen Receptor Signaling Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Checkpoints Pathway antibodies  Cell Cycle Checkpoints Pathway Diagram
 DNA Damage Response Pathway antibodies  DNA Damage Response Pathway Diagram
 G2/M Checkpoints Pathway antibodies  G2/M Checkpoints Pathway Diagram
 Regulation Of Telomerase Pathway antibodies  Regulation Of Telomerase Pathway Diagram

Diseases associated with anti-Rad9 antibody

 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >4 publications with Rad9 and Breast Neoplasms
 Lung Neoplasms Antibodies  >1 publications with Rad9 and Lung Neoplasms
 Skin Neoplasms Antibodies  >1 publications with Rad9 and Skin Neoplasms
 Neoplasms, Experimental Antibodies  >1 publications with Rad9 and Neoplasms, Experimental
 Neoplasms, Second Primary Antibodies  >1 publications with Rad9 and Neoplasms, Second Primary

Organs/Tissues associated with anti-Rad9 antibody

 Organ/Tissue Name  Pubmed Publications
 Testis Antibodies  >2 publications with Rad9 and Testis
 Skin Antibodies  >1 publications with Rad9 and Skin
 Lung Antibodies  >1 publications with Rad9 and Lung
 Intestine Antibodies  >1 publications with Rad9 and Intestine
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