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RAF Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6391903

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in… More details

$1165 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
RAF, Polyclonal Antibody
Full Product Name
RAF (Serine/Threonine-protein Kinase A-Raf, Proto-oncogene A-Raf-1, Proto-oncogene A-Raf, Proto-oncogene Pks, ARAF, ARAF1, PKS, PKS2) APC
Product Gene Name
anti-RAF antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human ARAF.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Immunogen
Full length human ARAF, aa1-606 (NP_001645.1).
Immunogen Sequence
MEPPRGPPANGAEPSRAVGTVKVYLPNKQRTVVTVRDGMSVYDSLDKALKVRGLNQDCCVVYRLIKGRKTVTAWDTAIAPLDGEELIVEVLEDVPLTMHNFVRKTFFSLAFCDFCLKFLFHGFRCQTCGYKFHQHCSSKVPTVCVDMSTNRQQFYHSVQDLSGGSRQHEAPSNRPLNELLTPQGPSPRTQHCDPEHFPFPAPANAPLQRIRSTSTPNVHMVSTTAPMDSNLIQLTGQSFSTDAAGSRGGSDGTPRGSPSPASVSSGRKSPHSKSPAEQRERKSLADDKKKVKNLGYRDSGYYWEVPPSEVQLLKRIGTGSFGTVFRGRWHGDVAVKVLKVSQPTAEQAQAFKNEMQVLRKTRHVNILLFMGFMTRPGFAIITQWCEGSSLYHHLHVADTRFDMVQLIDVARQTAQGMDYLHAKNIIHRDLKSNNIFLHEGLTVKIGDFGLATVKTRWSGAQPLEQPSGSVLWMAAEVIRMQDPNPYSFQSDVYAYGVVLYELMTGSLPYSHIGCRDQIIFMVGRGYLSPDLSKISSNCPKAMRRLLSDCLKFQREERPLFPQILATIELLQRSLPKIERSASEPSLHRTQADELPACLLSAARLVP
Conjugate
APC
Note
Preservative Free

Product Categories/Family for anti-RAF antibody

Antibodies; Protein Kinases
Applications Tested/Suitable for anti-RAF antibody
Western Blot (WB)
Application Notes for anti-RAF antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-RAF antibody

ARAF rabbit polyclonal antibody. Western Blot analysis of ARAF expression in human placenta.
Rabbit Anti-RAF Polyclonal Antibody – Cat# MBS6391903

Western Blot (WB) of anti-RAF antibody

Western Blot analysis of ARAF expression in transfected 293T cell line by ARAF polyclonal antibody. Lane 1: ARAF transfected lysate (67.6kD). Lane 2: Non-transfected lysate.
Rabbit Anti-RAF Polyclonal Antibody, Western Blot (WB) – Cat# MBS6391903

Testing Data of anti-RAF antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between ARAF and BRAF. HeLa cells were stained with ARAF rabbit purified polyclonal 1:1200 and BRAF mouse monoclonal antibody 1:50. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
Rabbit Anti-RAF Polyclonal Antibody, Testing Data – Cat# MBS6391903

Other Notes

Small volumes of anti-RAF antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RAF purchase

MBS6391903 is an antibody targeting RAF. Also known as RAF (Serine/Threonine-protein Kinase A-Raf, Proto-oncogene A-Raf-1, Proto-oncogene A-Raf, Proto-oncogene Pks, ARAF, ARAF1, PKS, PKS2) APC. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for RAF. It may not necessarily be applicable to this product.

NCBI GI #
4502192
NCBI GeneID
369
NCBI Accession #
NM_001654.1
UniProt Related Accession #
P10398
GenBank Nucleotide #
NM_001654.1
NCBI Official Full Name
Homo sapiens v-raf murine sarcoma 3611 viral oncogene homolog (ARAF), mRNA
NCBI Official Synonym Full Names
A-Raf proto-oncogene, serine/threonine kinase
NCBI Official Symbol
ARAF
NCBI Official Synonym Symbols
PKS2; A-RAF; ARAF1; RAFA1
Protein Family
Raf homolog serine/threonine-protein kinase
NCBI Protein Information
serine/threonine-protein kinase A-Raf

NCBI Summary for RAF

This proto-oncogene belongs to the RAF subfamily of the Ser/Thr protein kinase family, and maybe involved in cell growth and development. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2012]

Research Articles on RAF

1. The constitutive or induced re-localization of A-Raf to the plasma membrane compromises its ability to efficiently sequester and inactivate MST2, thus rendering cells susceptible to apoptosis

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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