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RANGAP1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6340239

RanGAP1, is a homodimeric 65-kD polypeptide that specifically induces the GTPase activity of RAN, but not of RAS by over 1,000-fold. RanGAP1 is the immediate antagonist of RCC1, a regulator molecule that keeps RAN in the active, GTP-bound state. The RANGAP1… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
RANGAP1, Polyclonal Antibody
Full Product Name
RANGAP1, NT (RANGAP1, KIAA1835, SD, Ran GTPase-activating protein 1) (PE)
Product Gene Name
Product Synonym Names
RANGAP1; KIAA1835; SD; Ran GTPase-activating protein 1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Chromosome Location
chromosome: 22; Location:
OMIM
602362. gene.
3D Structure
ModBase 3D Structure for P46060
Immunogen
RANGAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 19-49 amino acids from the N-terminal region of human RANGAP1.
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive

Product Categories/Family for anti-RANGAP1 antibody

Applications Tested/Suitable for anti-RANGAP1 antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes for anti-RANGAP1 antibody
IHC: 1:10-50
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-RANGAP1 antibody

RANGAP1 Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the RANGAP1 antibody detected the RANGAP1 protein (arrow).
Rabbit Anti-RANGAP1 Polyclonal Antibody – Cat# MBS6340239

Western Blot (WB) of anti-RANGAP1 antibody

Western blot analysis of RANGAP1 (arrow) using rabbit polyclonal RANGAP1 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RANGAP1 gene.
Rabbit Anti-RANGAP1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS6340239

Immunohistochemistry (IHC) of anti-RANGAP1 antibody

RANGAP1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RANGAP1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-RANGAP1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6340239

Product References and Citations for anti-RANGAP1 antibody

Zhang, J., et al. Biochem. Biophys. Res. Commun. 375(2):252-255(2008). Zuccolo, M., et al. EMBO J. 26(7):1853-1864(2007). Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007): Vertegaal, A.C., et al. Mol. Cell Proteomics 5(12):2298-2310(2006). Vertegaal, A.C., et al. Mol. Cell Proteomics 5(12):2298-2310(2006).

Other Notes

Small volumes of anti-RANGAP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RANGAP1 purchase

MBS6340239 is an antibody targeting RANGAP1. Also known as RANGAP1, NT (RANGAP1, KIAA1835, SD, Ran GTPase-activating protein 1) (PE). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for RANGAP1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
NCBI Official Full Name
ran GTPase-activating protein 1 [Homo sapiens]
NCBI Official Synonym Full Names
Ran GTPase activating protein 1
NCBI Official Symbol
RANGAP1
NCBI Official Synonym Symbols
SD; Fug1; KIAA1835; MGC20266; RANGAP1
UniProt Protein Name
Ran GTPase-activating protein 1
UniProt Gene Name
RANGAP1
UniProt Synonym Gene Names
KIAA1835; SD
UniProt Entry Name
RAGP1_HUMAN

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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