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RASSF8 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS647714

MBS647714 is an antibody targeting RASSF8. Also known as RASSF8, NT (RASSF8, C12orf2, Ras association domain-containing protein 8, Carcinoma-associated protein HOJ-1). Reactive in Human. Host: Rabbit. More details

$895 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
RASSF8, Polyclonal Antibody
Full Product Name
RASSF8, NT (RASSF8, C12orf2, Ras association domain-containing protein 8, Carcinoma-associated protein HOJ-1)
Product Gene Name
Product Synonym Names
Anti -RASSF8, NT (RASSF8, C12orf2, Ras association domain-containing protein 8, Carcinoma-associated protein HOJ-1)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
48,327 Da
Chromosome Location
Chromosome: 12; NC_000012.11 (26111964..26232825). Location: 12p12.3
OMIM
608180
3D Structure
ModBase 3D Structure for Q8NHQ8
Immunogen
RASSF8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 103-132 amino acids from the N-terminal region of human RASSF8.
Applications Tested/Suitable for anti-RASSF8 antibody
ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes for anti-RASSF8 antibody
Suitable for use in Western Blot, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Flow Cytometry: 1:10-50

Western Blot (WB) of anti-RASSF8 antibody

RASSF8 antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the RASSF8 antibody detected the RASSF8 protein (arrow).
Rabbit Anti-RASSF8 Polyclonal Antibody – Cat# MBS647714

Flow Cytometry (FC/FACS) of anti-RASSF8 antibody

RASSF8 antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-RASSF8 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS647714

Other Notes

Small volumes of anti-RASSF8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for RASSF8. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
ras association domain-containing protein 8 isoform b
NCBI Official Synonym Full Names
Ras association (RalGDS/AF-6) domain family (N-terminal) member 8
NCBI Official Symbol
RASSF8
NCBI Official Synonym Symbols
HOJ1; C12orf2
UniProt Protein Name
Ras association domain-containing protein 8
UniProt Synonym Protein Names
Carcinoma-associated protein HOJ-1
UniProt Gene Name
RASSF8
UniProt Synonym Gene Names
C12orf2
UniProt Entry Name
RASF8_HUMAN
NCBI Protein Information
ras association domain-containing protein 8; carcinoma-associated protein HOJ-1

NCBI Summary for RASSF8

This gene encodes a member of the Ras-assocation domain family (RASSF) of tumor suppressor proteins. This gene is essential for maintaining adherens junction function in epithelial cells and has a role in epithelial cell migration. It is a lung tumor suppressor gene candidate. A chromosomal translocation t(12;22)(p11.2;q13.3) leading to the fusion of this gene and the FBLN1 gene is found in a complex type of synpolydactyly. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2011]
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UniProt Comments for RASSF8

Tissue specificity: Widely expressed as a 6.2 kb transcript. A 2.2 kb alternatively spliced transcript is expressed exclusively in testis. Ref.7

Involvement in disease: A chromosomal aberration involving RASSF8 is found in a complex type of synpolydactyly referred to as 3/3-prime/4 synpolydactyly associated with metacarpal and metatarsal synostoses. Reciprocal translocation t(12;22)(p11.2;q13.3) with FBLN1.

Sequence similarities: Contains 1 Ras-associating domain.

Sequence caution: The sequence AAC95425.1 differs from that shown. Reason: Frameshift at position 108.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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