• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

RIOK3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1759003

The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring… More details

$210 / 0.01 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
RIOK3, Polyclonal Antibody
Full Product Name
Anti-RIOK3 Antibody Picoband
Product Gene Name
anti-RIOK3 antibody
Product Synonym Names
Nck-associated protein 1; Nck-associated protein 1; NAP 1; Membrane-associated protein HEM-2; p125Nap1; NCKAP1; HEM2, KIAA0587, NAP1
Clonality
Polyclonal
Isotype
Rabbit IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Purity/Purification
Immunogen affinity purified.
Specificity
Expressed in all tissues examined except peripheral blood leukocytes, with highest expression in brain, heart, and skeletal muscle.
Preparation and Storage
At -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.
Molecular Weight
58,671 Da
3D Structure
ModBase 3D Structure for O14730
Immunogen
E coli-derived human RIOK3 recombinant protein (Position: Q48-E519).
Subcellular Localization
Cell membrane, Single-pass membrane protein, Cytoplasmic side, Cell projection, lamellipodium membrane, Single-pass membrane protein, Cytoplasmic side, At the interface between the lamellipodial actin meshwork and the membrane.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500ug/ml.
Cross Reactivity
No cross-reactivity with other proteins.
Protein Function
Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. Actin remodeling activity is regulated by RAC1.

Product Categories/Family for anti-RIOK3 antibody

Primary Antibodies; Neurogenesis; Neurology Process; Neuroscience; NFKB Pathway; Nuclear Signaling; Signal Transduction; Signaling Pathway
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-RIOK3 antibody
Western Blot (WB), Flow Cytometry (FC/FACS), ELISA (EIA)
Application Notes for anti-RIOK3 antibody
WB: 0.25-0.5ug/ml, Human
FC/FACS (Fixed): 1-3ug/1x106 cells, Human
ELISA: 0.1-0.5ug/ml

Western Blot (WB) of anti-RIOK3 antibody

Figure 1. Western blot analysis of RIOK3 using anti-RIOK3 antibody (MBS1759003).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RIOK3 antigen affinity purified polyclonal antibody (#MBS1759003) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (#MBS176460) with Tanon 5200 system. A specific band was detected for RIOK3 at approximately 65 kDa. The expected band size for RIOK3 is at 59 kDa.
Rabbit Anti-RIOK3 Polyclonal Antibody – Cat# MBS1759003

Flow Cytometry (FC/FACS) of anti-RIOK3 antibody

Figure 2. Flow Cytometry analysis of HepG2 cells using anti-RIOK3 antibody (MBS1759003).
Overlay histogram showing HepG2 cells stained with MBS1759003 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RIOK3 Antibody (MBS1759003, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (MBS177405, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Rabbit Anti-RIOK3 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1759003

Product References and Citations for anti-RIOK3 antibody

1. Anaya, P., Evans, S. C., Dai, C., Lozano, G., May, G. S. Isolation of the Aspergillus nidulans sudD gene and its human homologue. Gene 211: 323-329, 1998.

Other Notes

Small volumes of anti-RIOK3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RIOK3 purchase

MBS1759003 is an antibody targeting RIOK3. Also known as Anti-RIOK3 Antibody Picoband. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for RIOK3. It may not necessarily be applicable to this product.

NCBI GI #
112180703
NCBI GeneID
8780
NCBI Accession #
AAH28360.1
UniProt Primary Accession #
O14730
UniProt Secondary Accession #
Q8IXN9
UniProt Related Accession #
O14730
NCBI Official Full Name
RIOK3 protein
NCBI Official Synonym Full Names
RIO kinase 3
NCBI Official Symbol
RIOK3
NCBI Official Synonym Symbols
SUDD
UniProt Protein Name
Serine/threonine-protein kinase RIO3
UniProt Synonym Protein Names
RIO kinase 3; sudD homolog
UniProt Gene Name
RIOK3
UniProt Synonym Gene Names
SUDD
NCBI Protein Information
serine/threonine-protein kinase RIO3

NCBI Summary for RIOK3

This gene was first identified by the similarity of its product to the Aspergillus nidulans SUDD protein. This gene is now recognized as a member of the right open reading frame (RIO) kinase gene family. This gene encodes a serine/threonine kinase that localizes to the cytoplasm and plays a role in the processing of the pre-40 S ribosomal subunit. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2017]
Show more

UniProt Comments for RIOK3

Involved in regulation of type I interferon (IFN)-dependent immune response which plays a critical role in the innate immune response against DNA and RNA viruses. May act as an adapter protein essential for the recruitment of TBK1 to IRF3 (PubMed:24807708). Phosphorylates IFIH1 on 'Ser-828' interfering with IFIH1 filament assembly on long dsRNA and resulting in attenuated IFIH1-signaling (PubMed:25865883). Can inhibit CASP10 isoform 7-mediated activation of the NF-kappaB signaling pathway (PubMed:19557502). May play a role in the biogenesis of the 40S ribosomal subunit. Involved in the processing of 21S pre-rRNA to the mature 18S rRNA (PubMed:22418843).
Show more

Research Articles on RIOK3

1. the ectopic expression of RIOK3 or a phosphomimetic MDA5-S828D mutation attenuated MDA5-mediated signaling.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-RIOK3 antibody

 Reference Product  PubMed Publications
 RIOK2 antibody  >1 publications with RIOK3 and RIOK2
 CASP10 antibody  >1 publications with RIOK3 and CASP10

Diseases associated with anti-RIOK3 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >3 publications with RIOK3 and Neoplasms
 Prostatic Diseases Antibodies  >1 publications with RIOK3 and Prostatic Diseases
 Necrosis Antibodies  >1 publications with RIOK3 and Necrosis
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000