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RPH3A Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS646568

Exocytosis of neurotransmitters and hormones is fundamental to synaptic neurotransmission and cell-cell communication. RAB3A (MIM 179390) is a small G protein that is thought to act at late stages of exocytosis, and RPH3A is a RAB3A effector (Lin et al., 2007… More details

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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
RPH3A, Polyclonal Antibody
Full Product Name
RPH3A, CT (RPH3A, KIAA0985, Rabphilin-3A, Exophilin-1)
Product Gene Name
Product Synonym Names
Anti -RPH3A, CT (RPH3A, KIAA0985, Rabphilin-3A, Exophilin-1)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
76,872 Da
Chromosome Location
Chromosome: 12; NC_000012.11 (113229343..113336686). Location: 12q24.13
OMIM
612159
3D Structure
ModBase 3D Structure for Q9Y2J0
Immunogen
RPH3A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 657-686 amino acids from the C-terminal region of human RPH3A.
Applications Tested/Suitable for anti-RPH3A antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-RPH3A antibody
Suitable for use in Western Blot, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500

Western Blot (WB) of anti-RPH3A antibody

RPH3A Antibody (C-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the RPH3A antibody detected the RPH3A protein (arrow).
Rabbit Anti-RPH3A Polyclonal Antibody – Cat# MBS646568

Other Notes

Small volumes of anti-RPH3A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RPH3A purchase

MBS646568 is an antibody targeting RPH3A. Also known as RPH3A, CT (RPH3A, KIAA0985, Rabphilin-3A, Exophilin-1). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for RPH3A. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
RPH3A protein
NCBI Official Synonym Full Names
rabphilin 3A homolog (mouse)
NCBI Official Symbol
RPH3A
UniProt Protein Name
Rabphilin-3A
UniProt Synonym Protein Names
Exophilin-1
Protein Family
UniProt Gene Name
RPH3A
UniProt Synonym Gene Names
KIAA0985
UniProt Entry Name
RP3A_HUMAN
NCBI Protein Information
rabphilin-3A; exophilin-1

UniProt Comments for RPH3A

Function: Protein transport. Probably involved with Ras-related protein Rab-3A in synaptic vesicle traffic and/or synaptic vesicle fusion. Could play a role in neurotransmitter release by regulating membrane flow in the nerve terminal

By similarity.

Subunit structure: Monomer. Interacts with RAB3A, RAB3B, RAB3C, RAB3D, RAB8A, RAB27A and RAB27B

By similarity.

Subcellular location: Cell junction › synapse

By similarity. Membrane; Peripheral membrane protein

By similarity.

Domain: Binds calcium via the C2 domains. The calcium-bound C2 domains mediate interactions with phospholipid bilayers

By similarity.

Sequence similarities: Contains 2 C2 domains.Contains 1 FYVE-type zinc finger.Contains 1 RabBD (Rab-binding) domain.

Sequence caution: The sequence BAA76829.2 differs from that shown. Reason: Erroneous initiation.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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